Rat 45 kDa calcium ELISA Kit | SDF4 elisa kit
Rat 45 kDa calcium-binding protein (SDF4) ELISA Kit
Reactivity
Rat
Synonyms
45 kDa calcium; N/A; Rat 45 kDa calcium-binding protein (SDF4) ELISA Kit; RP5-902P8.6; Cab45; calcium binding protein; SDF4 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Rabbit MMP-1. No significant cross-reactivity or interference between Rabbit MMP-1 and analogues was observed.
Sequence Length
362
Samples
Serum, Plasma And Other Biological Fluids
Assay Type
Quantitative Sandwich
Detection Range
3.12 pg/mL-200 pg/mL
Sensitivity
1.56 pg/mL (mean of 6 independent assays).
Intra-assay Precision
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for SDF4 elisa kit
Intended Uses: For the quantitative detection of Rabbit Matrix metalloproteinase 1 (MMP-1) concentration in serum, plasma and other biological fluids.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate MMP-1 in Rabbit serum, plasma. An antibody specific for MMP-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP-1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate MMP-1 in Rabbit serum, plasma. An antibody specific for MMP-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP-1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
44,350 Da
NCBI Official Full Name
45 kDa calcium-binding protein
NCBI Official Synonym Full Names
stromal cell derived factor 4
NCBI Official Symbol
Sdf4
NCBI Protein Information
45 kDa calcium-binding protein
UniProt Protein Name
45 kDa calcium-binding protein
UniProt Gene Name
Sdf4
UniProt Synonym Gene Names
Cab45; Cab45; SDF-4
Similar Products
Product Notes
The Rat SDF4 sdf4 (Catalog #AAA55784) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA55784 ELISA Kit recognizes Rat SDF4. It is sometimes possible for the material contained within the vial of "45 kDa calcium, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.