Search results for " Affinity Purified Rabbit Polyclonal Antibodies against Hepatitis Virus"

IHC (Immunohistchemistry) (Figure 5 Immunohistochemistry Validation of TIM-1Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-TIM-1 antibody (3809) at 10 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚ C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)

IF (Immunofluorescence) (Figure 5 Immunofluorescence Validation of TIM-1Immunofluorescent analysis of 4% paraformaldehyde-fixed human uterus cells labeling TIM-1 with 3809 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). Image showing both membrane and cytoplasmic staining on human uterus cells.)

WB (Western Blot) (Figure 4 Western Blot Validation in the Mouse Cell LineLoading: 15 μg of NIH/3T3 cell lysates.Antibodies: TIM-1 3809 (8 μg/mL), overnight incubation at 4˚ C in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)

WB (Western Blot) (Figure 3 Validation with TIM-1 siRNA KnockdownHeLa cells were transfected with control siRNAs (lane 1) or TIM-1 siRNAs (lane 2)Loading: 15 μg of HeLa whole cell lysates per lane.Antibodies: 3809 (8 μg/mL), 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)

WB (Western Blot) (Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 μg of lysates per lane.Antibodies: TIM-1 3809 (8 μg/mL), TIM-1 3811 (1 μg/mL), beta-actin (1 μg/mL), and GAPDH (0.02 μg/mL), overnight incubation at 4˚ C (3809) or 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)

WB (Western Blot) (Figure 1 Western Blot Validation in Human Cell LinesLoading: 15 μg of lysates per lane.Antibodies: TIM-1 3809 (8 μg/mL), overnight incubation at 4˚ C in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)

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4 results for " Affinity Purified Rabbit Polyclonal Antibodies against Hepatitis Virus" - showing 1-4

E1 (HCV), Polyclonal Antibody (Cat# AAA13708)

HBcAg (HBV), Polyclonal Antibody (Cat# AAA13719)

VP1, Polyclonal Antibody (Cat# AAA13727)

TIM-1, Polyclonal Antibody (Cat# AAA10935)

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