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9510 results for " Cell " - showing 9250-9300


Application Data (Staining of KG1 lymphocytes with Mouse anti Human CD59:FITC)

CD59, Monoclonal Antibody (Cat# AAA11917)

Full Name
MOUSE ANTI HUMAN CD59
Gene Names
CD59; 1F5; EJ16; EJ30; EL32; G344; MIN1; MIN2; MIN3; MIRL; HRF20; MACIF; MEM43; MIC11; MSK21; 16.3A5; HRF-20; MAC-IP; p18-20
Applications
EIA, EM, FC/FACS, IF, IP, WB
Pricing
IP (Immunoprecipitation) (Immunoprecipitating GFP in 293F whole cell lysate transfected with GFPLane 1: Mouse control IgG2b instead of AAA28064 in 293F whole cell lysate transfected with GFPLane 2: AAA28064 (4ug) + 293F whole cell lysate transfected with GFP (500ug)Lane 3: 293F whole cell lysate transfected with GFP (5ug)For western blotting, the blot was detected with AAA28064 at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:50000)

GFP, Monoclonal Antibody (Cat# AAA28064)

Full Name
GFP Monoclonal Antibody
Reactivity
All
Applications
EIA, WB, IF, FC/FACS, IP
Purity
>95%,Protein G purified
Pricing
FCM (Flow Cytometry) (Overlay histogram showing Hela cells stained with (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1ug/1*106cells) for 1 h at 4 degree C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4 degree C. Isotype control antibody (green line) was mouse IgG2b (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)

YWHAZ, Monoclonal Antibody (Cat# AAA27050)

Full Name
YWHAZ Monoclonal Antibody
Gene Names
YWHAZ; YWHAD; KCIP-1; 14-3-3-zeta
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, IHC, IF, FC/FACS
Purity
>95%, Protein A purified
Pricing
FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of THP-1 cells using anti-TLR1 antibody (AAA19134).Overlay histogram showing THP-1 cells stained with AAA19134 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TLR1 Antibody (AAA19134,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

TLR1, Polyclonal Antibody (Cat# AAA19134)

Full Name
Anti-TLR1 Picoband antibody
Gene Names
TLR1; TIL; CD281; rsc786; TIL. LPRS5
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, FC/FACS, IHC, ICC, WB
Pricing
IHC (Immunohistchemistry) (Figure 6. IHC analysis of Flt3 / CD135 using anti-Flt3 / CD135 antibody (AAA19130).Flt3 / CD135 was detected in paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Flt3 / CD135 Antibody (AAA19130) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

Flt3/CD135, Polyclonal Antibody (Cat# AAA19130)

Full Name
Anti-Flt3/CD135 Picoband Antibody
Reactivity
Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
IHC (Immunohistochemistry) (Figure 8. IHC analysis of beta Catenin using anti-beta Catenin antibody (AAA19129).beta Catenin was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-beta Catenin Antibody (AAA19129) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

beta Catenin, Polyclonal Antibody (Cat# AAA19129)

Full Name
Anti-beta Catenin Picoband Antibody
Gene Names
CTNNB1; EVR7; CTNNB; MRD19; armadillo
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
IHC (Immunohistchemistry) (Figure 6. IHC analysis of HP1 gamma using anti-HP1 gamma antibody (AAA19145).HP1 gamma was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HP1 gamma Antibody (AAA19145) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

HP1 gamma, Polyclonal Antibody (Cat# AAA19145)

Full Name
Anti-HP1 gamma Picoband antibody
Gene Names
CBX3; HECH; HP1-GAMMA; HP1Hs-gamma
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
IHC (Immunohistchemistry) (Figure 6. IHC analysis of Cyclophilin B using anti-Cyclophilin B antibody (AAA19167).Cyclophilin B was detected in paraffin-embedded section of rat thyroid gland tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Cyclophilin B Antibody (AAA19167) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

Cyclophilin B, Polyclonal Antibody (Cat# AAA19167)

Full Name
Anti-Cyclophilin B Picoband Antibody
Gene Names
PPIB; OI9; CYPB; SCYLP; CYP-S1; HEL-S-39
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
IHC (Immunohistchemistry) (At 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

NDEL1, Polyclonal Antibody (Cat# AAA31462)

Full Name
Phospho-NDEL1 (Thr219) Antibody
Gene Names
NDEL1; EOPA; NDE2; NUDEL; MITAP1; NDE1L1
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Applications
WB, IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data (Published customer image:Phycoerythrin conjugated Mouse anti Human CD83 antibody, clone HB15e used for the evaluation of CD83 expression on monocyte derived dendritic cells by flow cytometry.Phenotypic characterization of immunogenic and tolerogenic moDC populations by flow cytometry. Monocytes were negatively selected from PBMC using magnetic beads. Immature moDC were generated with IL-4 and GM-CSF for 6 days. 15d-PGJ2 (PGJ2 DC) and dexamethasone plus 1alpha,25-dihydroxyvitamin were added to generate tolerogenic moDC, respectively (PGJ2 DC and Dex/VD3 DC). To generate immunogenic moDC, immature moDC were stimulated for 24 h with LPS, polyI:C and a cytokine cocktail containing TNF-alpha, IL-1beta, IL-6 and PGE2, respectively. The phenotypes of the cells were analyzed by flow cytometry. Live cells were gated according to FSC/SSC. One representative experiment out of three is shown.From: Sprater F, Hovden A-O, Appel S (2012)Expression of ESE-3 Isoforms in Immunogenic and Tolerogenic Human Monocyte-Derived Dendritic Cells.PLoS ONE 7(11): e49577.)

CD83, Monoclonal Antibody (Cat# AAA12280)

Full Name
MOUSE ANTI HUMAN CD83
Reactivity
Human
Applications
FC/FACS, IHC, IHC, IP
Pricing
Application Data (Published customer image:Phycoerythrin conjugated Mouse anti Human CD83 antibody, clone HB15e used for the evaluation of CD83 expression on monocyte derived dendritic cells by flow cytometry.Phenotypic characterization of immunogenic and tolerogenic moDC populations by flow cytometry. Monocytes were negatively selected from PBMC using magnetic beads. Immature moDC were generated with IL-4 and GM-CSF for 6 days. 15d-PGJ2 (PGJ2 DC) and dexamethasone plus 1alpha,25-dihydroxyvitamin were added to generate tolerogenic moDC, respectively (PGJ2 DC and Dex/VD3 DC). To generate immunogenic moDC, immature moDC were stimulated for 24 h with LPS, polyI:C and a cytokine cocktail containing TNF-alpha, IL-1beta, IL-6 and PGE2, respectively. The phenotypes of the cells were analyzed by flow cytometry. Live cells were gated according to FSC/SSC. One representative experiment out of three is shown.From: Sprater F, Hovden A-O, Appel S (2012)Expression of ESE-3 Isoforms in Immunogenic and Tolerogenic Human Monocyte-Derived Dendritic Cells.PLoS ONE 7(11): e49577.)

CD83, Monoclonal Antibody (Cat# AAA12283)

Full Name
MOUSE ANTI HUMAN CD83
Reactivity
Human
Applications
FC/FACS, IHC, IHC
Pricing
IHC (Immunohistochemistry) (At 1/100 staining Human mammary cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

SAMHD1, Polyclonal Antibody (Cat# AAA31278)

Full Name
Phospho-SAMHD1 (Thr592) Antibody
Gene Names
SAMHD1; DCIP; CHBL2; HDDC1; MOP-5; SBBI88
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
FCM (Flow Cytometry) (SOCS1 Antibody (N-term) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

SOCS1, Polyclonal Antibody (Cat# AAA28752)

Full Name
SOCS1 Antibody (N-term)
Gene Names
SOCS1; JAB; CIS1; SSI1; TIP3; CISH1; SSI-1; SOCS-1
Reactivity
Mouse, rat
Applications
WB, EIA, IHC, IF, FC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
SDS-PAGE (SDS Page analysis of purified S100B Mouse Monoclonal Antibody (S100B/1012).)

S100B, Monoclonal Antibody (Cat# AAA23893)

Full Name
S100B (Astrocyte and Melanoma Marker)
Gene Names
S100B; NEF; S100; S100-B; S100beta
Reactivity
Human, Mouse, Rat, Cow. Others not known.
Applications
FC/FACS, IF, WB, IHC
Pricing
Application Data (Analysis of Protein Array containing more than 19, 000 full-length human proteins using Oct-2 Mouse Monoclonal Antibody (OCT2/2137) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

OCT-2 (POU2F2), Monoclonal Antibody (Cat# AAA23902)

Full Name
OCT-2 (POU2F2) (B-Cell Marker)
Gene Names
POU2F2; OCT2; OTF2; Oct-2
Reactivity
Human. Others not known.
Applications
EIA, WB, IHC
Pricing
Application Data (Published customer image: Representative images of the inflammatory changes in the facial nucleus during axonal regeneration, one week following facial nerve transaction. a, b: CD11b immunoreactivity for microglia is increased in the axotomized facial nucleus, and microglia enwrap the facial motor neurons, e.g. at arrows. The regenerating neurons were retrogradely labelled with fluorogold. c, d: CD6- positive T-cells accumulated in the injured motor nucleus (arrows). They had little cytoplasm but dense nuclei (c) and were sometimes clustered around neurons retrogradely labelled with fluorogold (d). The scale bar in (a) also applies to (b) and that in (c) also applies to (d).From: Shokouhi et al. BMC Neuroscience 2010 11:13.)

CD11b, Monoclonal Antibody (Cat# AAA11876)

Full Name
MOUSE ANTI RAT CD11b:FITC
Gene Names
ITGAM; CD11B
Applications
FC/FACS
Pricing
Application Data (Analysis of Protein Array containing more than 19,000 full-length human proteins using Cytokeratin 15 Mouse Monoclonal Antibody (KRT15/2957). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

Cytokeratin 15, Monoclonal Antibody (Cat# AAA23923)

Full Name
Cytokeratin 15 (Esophageal Squamous Cell Carcinoma Marker)
Gene Names
KRT15; K15; CK15; K1CO
Reactivity
Human
Applications
FC/FACS, IF, WB, IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
IHC (Immunohistchemistry) (AAA31095 at 1/100 staining Human kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Histone H3, Polyclonal Antibody (Cat# AAA31095)

Full Name
Histone H3 Antibody
Gene Names
HIST1H3A; H3/A; H3FA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of U-87 cells using anti-SSH3BP1 antibody (AAA11656).Overlay histogram showing U-87 cells stained with AAA11656 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SSH3BP1 Antibody (AAA11656,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

SSH3BP1, Polyclonal Antibody (Cat# AAA11656)

Full Name
Anti-SSH3BP1 Antibody
Gene Names
ABI1; E3B1; ABI-1; ABLBP4; NAP1BP; SSH3BP; SSH3BP1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Immunogen Affinity Purified
Pricing
FCM (Flow Cytometry) (Overlay histogram showing Hela cells stained with CSB-MA878942A1m (red line) at 1:300. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4 degree C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4 degree C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.)

PD-L1, Monoclonal Antibody (Cat# AAA27017)

Full Name
PD-L1 Monoclonal Antibody
Gene Names
CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
Reactivity
Human
Applications
EIA, WB, IHC, IF, FC/FACS
Purity
>95%
Protein G Purified
Pricing
ICC (Immunocytochemistry) (Figure 7. IHC analysis of ERAB using anti-ERAB antibody (AAA11668).ERAB was detected in immunocytochemical section of SMMC-7721 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ERAB Antibody (AAA11668) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

ERAB, Polyclonal Antibody (Cat# AAA11668)

Full Name
Anti-ERAB Antibody
Gene Names
HSD17B10; ABAD; CAMR; ERAB; HCD2; MHBD; HADH2; MRPP2; MRX17; MRX31; SCHAD; MRXS10; SDR5C1; 17b-HSD10; DUPXp11.22
Reactivity
Human, Mouse
Applications
WB, IHC
Purity
Immunogen Affinity Purified
Pricing
WB (Western Blot) (Sample: Recombinant GAL3, Human; Antibody: Rabbit Anti-Human GAL3 Ab)

Galectin 3 (GAL3), Active Protein (Cat# AAA21140)

Full Name
Active Galectin 3 (GAL3)
Gene Names
LGALS3; L31; GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2
Reactivity
Homo sapiens (Human)
Applications
Cell culture; Activity Assays.
Purity
>98%
Pricing
Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)

F4/80, Monoclonal Antibody (Cat# AAA12174)

Full Name
RAT ANTI MOUSE F4/80
Gene Names
Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7
Applications
EM, FC/FACS, IF, IP, RIA, RE, WB
Pricing
Application Data

TCP1 delta, Polyclonal Antibody (Cat# AAA11672)

Full Name
Anti-TCP1 delta Antibody
Gene Names
CCT4; SRB; Cctd; CCT-DELTA
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
WB, IHC
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of MCF-7 cells using anti-CHRNA5 antibody (AAA11685).Overlay histogram showing MCF-7 cells stained with AAA11685 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CHRNA5 Antibody (AAA11685,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

CHRNA5, Polyclonal Antibody (Cat# AAA11685)

Full Name
Anti-CHRNA5 Antibody
Gene Names
CHRNA5; LNCR2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistochemistry) (At 1/200 staining Human lung cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

Caveolin 2, Polyclonal Antibody (Cat# AAA31397)

Full Name
Phospho-Caveolin 2 (Ser36) Antibody
Gene Names
CAV2; CAV
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data (At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

LAT, Polyclonal Antibody (Cat# AAA31413)

Full Name
Phospho-LAT (Tyr161) Antibody
Gene Names
LAT; LAT1; pp36
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (91%), Zebrafish (83%), Bovine (100%), Horse (91%), Sheep (100%), Rabbit (80%), Dog (82%)
Applications
WB, IHC, IF, ICC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data (At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

p70 S6 Kinase, Polyclonal Antibody (Cat# AAA31408)

Full Name
Phospho-p70 S6 Kinase (Ser427) Antibody
Gene Names
RPS6KB1; S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Applications
WB, IHC, IF, ICC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD25 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. High power)

CD25, Monoclonal Antibody (Cat# AAA11965)

Full Name
MOUSE ANTI RAT CD25
Gene Names
Il2ra; IL2RAC
Applications
EIA, FC/FACS, IP
Pricing
FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of Hela cells using anti-PDE4D antibody (AAA19143).Overlay histogram showing Hela cells stained with AAA19143 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PDE4D Antibody (AAA19143,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

PDE4D, Polyclonal Antibody (Cat# AAA19143)

Full Name
Anti-PDE4D Picoband antibody
Gene Names
PDE4D; DPDE3; PDE43; STRK1; ACRDYS2; HSPDE4D; PDE4DN2
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, FC/FACS, IHC, ICC, WB
Pricing
Application Data (Published customer image: Representative images of the inflammatory changes in the facial nucleus during axonal regeneration, one week following facial nerve transaction. a, b: CD11b immunoreactivity for microglia is increased in the axotomized facial nucleus, and microglia enwrap the facial motor neurons, e.g. at arrows. The regenerating neurons were retrogradely labelled with fluorogold. c, d: CD6- positive T-cells accumulated in the injured motor nucleus (arrows). They had little cytoplasm but dense nuclei (c) and were sometimes clustered around neurons retrogradely labelled with fluorogold (d). The scale bar in (a) also applies to (b) and that in (c) also applies to (d).From: Shokouhi et al. BMC Neuroscience 2010 11:13.)

CD11b, Monoclonal Antibody (Cat# AAA11970)

Full Name
MOUSE ANTI RAT CD11b
Gene Names
ITGAM; CD11B
Applications
FC/FACS, IF, IP
Pricing
IHC (Immunohistochemistry) (Figure 7. IHC analysis of MYLK using anti-MYLK antibody (AAA19154).MYLK was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MYLK Antibody (AAA19154) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )

MYLK, Polyclonal Antibody (Cat# AAA19154)

Full Name
Anti-MYLK Picoband antibody
Gene Names
MYLK; KRP; AAT7; MLCK; MLCK1; MYLK1; smMLCK; MLCK108; MLCK210; MSTP083
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
Application Data (At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

Caspase 3, Polyclonal Antibody (Cat# AAA31443)

Full Name
Phospho-Caspase 3 (Ser26) Antibody
Gene Names
CASP3; CPP32; SCA-1; CPP32B
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Rabbit (88%)
Applications
WB, IHC, IF, ICC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data (Publised customer image:Mouse anti Human CD163 antibody, clone EDHu-1 used for the identification of perivascular macrophages in human brain by immunofluorescence.Image caption:Images demonstrating immunohistological stainings of amylin and double immunofluorescence staining against NG2/amylin, laminin/amylin and CD163/amylin in the hippocampus of the patient with AD and T2D.Amylin cell inclusions are indicated with arrows in (a) and shown in a higher magnification in (b). Pericytes with round cell bodies and NG2-positive coverage of the microvessel surface (green in c), without amylin cell inclusions (red in d), displayed round DAPI-positive cell nuclei (blue in e). The images in (c), (d) and (e) are merged in (f). Cells with more diffuse and weak NG2 staining (indicated by the arrowhead, green in g) and cytosolic amylin cell inclusions (red in h) showed altered cell nuclei (indicated with arrow, blue in i).The adjacent unaffected NG2-positive cell is indicated with an arrowhead in (i). The images in (g), (h) and (i) are merged in (j). Cells enclosed by laminin (green in K) contained amylin grains (red in l) and fragmented DAPI-positive cell nuclei (indicated with an arrow blue in m). The images in (k), (l) and (m) are merged in (n).Loss of NG2 coverage (green in o) was associated with polarized amylin cell inclusion (red in p) and fragmented DAPI-positive cell nuclei (indicated with an arrow, blue in q). The images in (o), (p) and (q) are merged in (r). Staining against macrophage marker CD163 (green in s) did not co-localize with amylin cell inclusions (red in t). The cell nucleus was stained with DAPI (blue in U). The images in (s), (t) and (u) are merged in (v). Scale bars: (a) 50 mum, (b), (c) to (v) 5 mum.From: Schultz, N. et al. (2016).Amylin alters human brain pericyte viability and NG2 expression.J Cereb Blood Flow & Metab. Jun 28 [Epub ahead of print]This is from an open access article distributed under the terms of the Creative Commons Attribution License.)

CD163, Monoclonal Antibody (Cat# AAA12256)

Full Name
Mouse Anti Human CD163: RPE
Gene Names
CD163; M130; MM130; SCARI1
Reactivity
Human
Applications
FC/FACS
Pricing
IHC (Immunohistochemistry) (At 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IRF7, Polyclonal Antibody (Cat# AAA31458)

Full Name
Phospho-IRF7 (Ser477) Antibody
Gene Names
IRF7; IRF7A; IRF7B; IRF7C; IRF7H; IRF-7H
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%)
Applications
WB, IHC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

PDHA1/2, Polyclonal Antibody (Cat# AAA31464)

Full Name
Phospho-PDHA1/2 (Ser293/Ser291) Antibody
Gene Names
PDHA1; PDHA; PHE1A; PDHCE1A
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Chicken (100%), Xenopus (100%)
Applications
WB, IHC, IF, ICC, EIA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Pricing
Strength of Signal (Analysis of Protein Array containing more than 19,000 full-length human proteins using Vimentin (VIM) Mouse Monoclonal Antibody (VM452)Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

Vimentin, Monoclonal Antibody (Cat# AAA13810)

Full Name
Vimentin (Mesenchymal Cell Marker) Mouse Monoclonal Antibody
Gene Names
VIM; HEL113; CTRCT30
Reactivity
Human, Cow, Dog, Cat, Pig, Goat, Chicken.
Does not react with Mouse and Rat
Applications
FC/FACS, IF, WB, IHC
Pricing
FCM (Flow Cytometry) (Figure 11. Flow Cytometry analysis of U937 cells using anti-Coronin 1a/TACO/CORO1A antibody (AAA19291).Overlay histogram showing U937 cells stained with AAA19291 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Coronin 1a/TACO/CORO1A Antibody (AAA19291, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

Coronin 1a/TACO/CORO1A, Polyclonal Antibody (Cat# AAA19291)

Full Name
Anti-Coronin 1a/TACO/CORO1A Antibody
Gene Names
CORO1A; p57; TACO; CLABP; HCORO1; CLIPINA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of A549 cells using anti-DR6/TNFRSF21 antibody (AAA19292).Overlay histogram showing A549 cells stained with AAA19292 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DR6/TNFRSF21 Antibody (AAA19292, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

DR6/TNFRSF21, Polyclonal Antibody (Cat# AAA19292)

Full Name
Anti-DR6/TNFRSF21 Antibody
Gene Names
TNFRSF21; DR6; CD358; BM-018
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
IF (Immunofluorescence) (Figure 6. IF analysis of SCG10/STMN2 using anti- SCG10/STMN2 antibody (AAA19299).SCG10/STMN2 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- SCG10/STMN2 Antibody (AAA19299) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

SCG10/STMN2, Polyclonal Antibody (Cat# AAA19299)

Full Name
Anti-SCG10/STMN2 Antibody
Gene Names
STMN2; SCG10; SCGN10
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of CACO-2 cells using anti-Glycine decarboxylase/GLDC antibody (AAA19300).Overlay histogram showing CACO-2 cells stained with AAA19300 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Glycine decarboxylase/GLDC Antibody (AAA19300, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

Glycine decarboxylase/GLDC, Polyclonal Antibody (Cat# AAA19300)

Full Name
Anti-Glycine decarboxylase/GLDC Antibody
Gene Names
GLDC; GCE; GCSP; HYGN1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of U20S cells using anti-VDAC3 antibody (AAA19301).Overlay histogram showing U20S cells stained with AAA19301 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-VDAC3 Antibody (AAA19301, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

VDAC3, Polyclonal Antibody (Cat# AAA19301)

Full Name
Anti-VDAC3 Antibody
Gene Names
VDAC3; VDAC-3; HD-VDAC3
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of 293T cells using anti-CDC45L antibody (AAA19360).Overlay histogram showing 293T cells stained with AAA19360 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- CDC45L Antibody (AAA19360, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

CDC45L, Monoclonal Antibody (Cat# AAA19360)

Full Name
Anti-CDC45L Antibody (monoclonal, 6H6)
Gene Names
CDC45; CDC45L; CDC45L2; PORC-PI-1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
IHC (Immunohistchemistry) (Validation of PKR in Rat Lung Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-PKR antibody (AAA10917) at 2.5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 degree C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)

PKR, Polyclonal Antibody (Cat# AAA10917)

Full Name
PKR Antibody
Gene Names
EIF2AK2; PKR; PRKR; EIF2AK1; PPP1R83
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, IHC, IF
Purity
PKR Antibody is affinity chromatography purified via peptide column.
Pricing
FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of SiHa cells using anti- ASS1 antibody (AAA19369).Overlay histogram showing SiHa cells stained with AAA19369 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-ASS1 Antibody (AAA19369, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

ASS1, Monoclonal Antibody (Cat# AAA19369)

Full Name
Anti-ASS1 Antibody (monoclonal, 5I5)
Gene Names
ASS1; ASS; CTLN1
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of SiHa cells using anti- ASS1 antibody (AAA19370).Overlay histogram showing SiHa cells stained with AAA19370 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-ASS1 Antibody (AAA19370, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

ASS1, Monoclonal Antibody (Cat# AAA19370)

Full Name
Anti-ASS1 Antibody (monoclonal, 7I9)
Gene Names
ASS1; ASS; CTLN1
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of A431 cells using anti-HIF-1 alpha/HIF1A antibody (AAA19213).Overlay histogram showing A431 cells stained with AAA19213 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HIF-1 alpha/HIF1A Antibody (AAA19213, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

HIF-1 alpha/HIF1A, Polyclonal Antibody (Cat# AAA19213)

Full Name
Anti-HIF-1 alpha/HIF1A Antibody
Gene Names
HIF1A; HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of Raji cells using anti-CD38 antibody (AAA19217).Overlay histogram showing Raji cells stained with AAA19217 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD38 Antibody (AAA19217, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

CD38, Polyclonal Antibody (Cat# AAA19217)

Full Name
Anti-CD38 Antibody
Gene Names
CD38; T10; ADPRC 1
Reactivity
Human
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of HepG2 cells using anti-MERTK antibody (AAA19219).Overlay histogram showing HepG2 cells stained with AAA19219 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MERTK Antibody (AAA19219, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

MERTK, Polyclonal Antibody (Cat# AAA19219)

Full Name
Anti-MERTK Antibody
Gene Names
MERTK; MER; RP38; c-mer
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of U20S cells using anti- ADA antibody (AAA19184). Overlay histogram showing U20S cells stained with AAA19184 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- ADA Antibody (AAA19184, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG (BA1126, 5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

ADA, Monoclonal Antibody (Cat# AAA19184)

Full Name
Anti-ADA Antibody (monoclonal, 6D4)
Reactivity
Human
Applications
WB, IHC, FC/FACS
Purity
Immunogen Affinity Purified
Pricing

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