Search results for " Inflammation"

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Bioactivity (Neutrophil elastase (NE), also known as polymorphonuclear leukocyte elastase, is a major protease in the primary granules of neutrophils, is involved in microbicidal activity. It is located primarily in the azurophil granules of polymorphonuclear leukocytes. NE is an important factor promoting inflammation, has bactericidal effects, and shortens the inflammatory process. NE also regulates tumor growth by promoting metastasis and tumor microenvironment remodeling. However, NE plays a role in killing tumors under certain conditions and promotes other diseases such as pulmonary ventilation dysfunction. Additionally, it plays a complex role in various physiological processes and mediates several diseases. Lactoferrin (LTF) has been identified as an interactor of NE, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant rat NE and recombinant goat LTF. Briefly, biotin-linked NE were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to LTF-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 ul stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant rat NE and recombinant goat LTF was shown in Figure 1, the EC50 for this effect is 1.19 ug/mL.)

Neutrophil Elastase (NE), Active Protein (Cat# AAA161713)

Full Name

Active Neutrophil Elastase (NE)

Gene Names

Elane; Ela2

Applications

Activity Assay, Cell Culture

Purity

Greater than 90% by SDS-PAGE

Pricing

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FCM/FACS (Flow Cytometry) (Figure 3. Flow Cytometry analysis of HepG2 cells using anti-RPS14 antibody (AAA127195).Overlay histogram showing HepG2 cells stained with AAA127195 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RPS14 Antibody (AAA127195, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

RPS14, Polyclonal Antibody (Cat# AAA127195)

Full Name

Anti-RPS14 Antibody Picoband

Gene Names

RPS14; S14; EMTB

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

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c-KIT (D820Y), Recombinant Protein (Cat# AAA73629)

Full Name

c-KIT (D820Y), Active

Applications

Kinase Assay

Pricing

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c-MER, Recombinant Protein (Cat# AAA73688)

Full Name

c-MER, Active

Applications

Western Blot

Pricing

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TIE2 (L914F), Recombinant Protein (Cat# AAA73994)

Full Name

TIE2 (L914F), Active

Applications

Kinase Assay

Pricing

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PTPN1 (PTP1B), Recombinant Protein (Cat# AAA73809)

Full Name

PTPN1 (PTP1B), Active

Applications

Western Blot

Pricing

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DAPK1, Recombinant Protein (Cat# AAA73389)

Full Name

DAPK1, Active

Applications

Western Blot

Pricing

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BIRC3, Recombinant Protein (Cat# AAA73282)

Full Name

BIRC3, Active

Applications

Western Blot

Pricing

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YES1, Recombinant Protein (Cat# AAA74118)

Full Name

YES1, Active

Applications

Western Blot

Pricing

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HDAC3/NCOR2, Recombinant Protein (Cat# AAA73599)

Full Name

HDAC3/NCOR2, Active

Applications

Western Blot, Histone Deacetylase Assay

Pricing

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HDAC4, Recombinant Protein (Cat# AAA73600)

Full Name

HDAC4, Active

Applications

Western Blot, Histone Deacetylase Assay

Purity

>95%

Pricing

CD97, ELISA Kit (Cat# AAA95028)

Full Name

Monkey CD97 ELISA Kit

Gene Names

CD97; TM7LN1

Reactivity

Monkey

Pricing

TLR5, Polyclonal Antibody (Cat# AAA75760)

Full Name

Rabbit TLR5 Antibody

Gene Names

TLR5; SLE1; TIL3; SLEB1; MELIOS

Reactivity

Cat, Human, Mouse, Pig

Applications

Western Blot, Immunoprecipitation

Purity

Affinity Purified

Pricing

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IHC (Immunohistochemisry) (Figure 3. IHC analysis of PF4 using anti-PF4 antibody (AAA124596).PF4 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PF4 Antibody (AAA124596) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

PF4/Cxcl4, Polyclonal Antibody (Cat# AAA124596)

Full Name

Anti-PF4/Cxcl4 Picoband antibody

Gene Names

Pf4; PF-4; Pf4a; Cxcl4; RATPF4A

Reactivity

Mouse, Rat
No cross reactivity with other proteins.

Applications

Western Blot, Immunohistochemistry

Pricing

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alpha-L-Fucosidase (AFU), Assay Kit (Cat# AAA178116)

Full Name

alpha-L-Fucosidase (AFU) Acitivity Assay Kit

Pricing

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Application Data (Published customer image: Assessment of dectin-2 function on inflammatory cells. Assessment of the impact of blockade of dectin-2 (D2.11E4) on the recognition (upper graphs) and response to (lower graphs) zymosan of inflammatory neutrophils and monocyte/M˜. Data represents mean+/-SEM of triplicates from a representative of 3 independent experiments.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)

DECTIN-2, Monoclonal Antibody (Cat# AAA49373)

Full Name

RAT ANTI MOUSE DECTIN-2

Gene Names

Clec4n; Nkcl; Clec6a; Clecsf10

Applications

Immunoprecipitation, Flow Cytometry, Immunohistochemistry

Pricing

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c-KIT (d557-558), Recombinant Protein (Cat# AAA73637)

Full Name

c-KIT (d557-558), Active

Applications

Kinase Assay

Pricing

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HDAC4, Recombinant Protein (Cat# AAA73601)

Full Name

HDAC4, Active

Applications

Western Blot, Histone Deacetylase Assay

Pricing

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PTPN12 (PTP-PEST), Recombinant Protein (Cat# AAA73830)

Full Name

PTPN12 (PTP-PEST), Active

Applications

Western Blot

Pricing

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ERN1 (IRE1), Recombinant Protein (Cat# AAA73528)

Full Name

ERN1 (IRE1), Active

Applications

Western Blot

Pricing

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TAK1-TAB1, Recombinant Protein (Cat# AAA73662)

Full Name

TAK1-TAB1, Active

Applications

Western Blot

Pricing

CD49d/VLA-4, Antibody (Cat# AAA78632)

Full Name

Mouse Anti-Human CD49d/VLA-4-PE

Reactivity

Human

Applications

Flow Cytometry

Pricing

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Application Data (Analysis of Protein Array containing more than 19,000 full-length human proteins using TIM-3 Mouse Monoclonal Antibody (TIM3/3113) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

TIM3/HAVCR2/CD366, Monoclonal Antibody (Cat# AAA215270)

Full Name

TIM3/HAVCR2/CD366 (Effector T-Cell Marker)

Gene Names

HAVCR2; TIM3; CD366; KIM-3; SPTCL; TIMD3; Tim-3; TIMD-3; HAVcr-2

Reactivity

Human

Applications

Immunohistochemistry

Purity

Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Pricing

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CD97, Antibody (Cat# AAA129339)

Full Name

Anti-Hu CD97 PE

Gene Names

CD97; TM7LN1

Reactivity

Human

Applications

Flow Cytometry

Purity

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Pricing

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IHC (Immunohistochemistry) (Figure 4. IHC analysis of Renin/REN using anti-Renin/REN antibody (AAA127194).Renin/REN was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Renin/REN Antibody (AAA127194) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

Renin/REN, Polyclonal Antibody (Cat# AAA127194)

Full Name

Anti-Renin/REN Antibody Picoband

Gene Names

REN; HNFJ2

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

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CK1 delta, Recombinant Protein (Cat# AAA73375)

Full Name

CK1 delta, Active

Applications

Western Blot

Pricing

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CK1 gamma 1, Recombinant Protein (Cat# AAA73380)

Full Name

CK1 gamma 1, Active

Applications

Western Blot

Pricing

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PDGFR alpha (E675G Y676C), Recombinant Protein (Cat# AAA73798)

Full Name

PDGFR alpha (E675G Y676C), Active

Pricing

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CK1 alpha 1L, Recombinant Protein (Cat# AAA73384)

Full Name

CK1 alpha 1L, Active

Gene Names

CSNK1A1L; CK1

Applications

Western Blot

Pricing

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STK3, Recombinant Protein (Cat# AAA73959)

Full Name

STK3, Active

Gene Names

STK3; KRS1; MST2

Applications

Western Blot

Pricing

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Application Data (Published customer image: Assessment of dectin-2 function on inflammatory cells. Assessment of the impact of blockade of dectin-2 (D2.11E4) on the recognition (upper graphs) and response to (lower graphs) zymosan of inflammatory neutrophils and monocyte/M˜. Data represents mean+/-SEM of triplicates from a representative of 3 independent experiments.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)

DECTIN-2, Monoclonal Antibody (Cat# AAA49674)

Full Name

RAT ANTI MOUSE DECTIN-2

Gene Names

Clec4n; Nkcl; Clec6a; Clecsf10

Applications

Immunoprecipitation, Flow Cytometry, Immunohistochemistry

Pricing

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SRPK2, Recombinant Protein (Cat# AAA73958)

Full Name

SRPK2, Active

Applications

Western Blot

Pricing

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PTPN2 (TC-PTP), Recombinant Protein (Cat# AAA73810)

Full Name

PTPN2 (TC-PTP), Active

Applications

Western Blot

Pricing

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CK1 gamma 2, Recombinant Protein (Cat# AAA73378)

Full Name

CK1 gamma 2, Active

Applications

Western Blot

Pricing

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CK1 epsilon (R178C), Recombinant Protein (Cat# AAA73377)

Full Name

CK1 epsilon (R178C), Active

Applications

Kinase Assay

Pricing

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TAOK1, Recombinant Protein (Cat# AAA74069)

Full Name

TAOK1, Active

Applications

Western Blot

Pricing

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ABL1 (E255K), Recombinant Protein (Cat# AAA73189)

Full Name

ABL1 (E255K), Active

Applications

Kinase Assay

Pricing

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FGFR1 (FLT2), Recombinant Protein (Cat# AAA73536)

Full Name

FGFR1 (FLT2), Active

Applications

Western Blot

Pricing

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TYRO3, Recombinant Protein (Cat# AAA74068)

Full Name

TYRO3, Active

Applications

Western Blot

Pricing

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YES1 (T348I), Recombinant Protein (Cat# AAA74119)

Full Name

YES1 (T348I), Active

Applications

Kinase Assay

Pricing

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Cluster Of Differentiation 97 (CD97), Monoclonal Antibody (Cat# AAA152699)

Full Name

Monoclonal Antibody to Cluster Of Differentiation 97 (CD97)

Gene Names

CD97; TM7LN1

Reactivity

Human, Pig

Applications

Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Affinity Chromatography

Pricing

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Bioactivity (Inhibitor Of Kappa-Light Polypeptide Gene Enhancer In B-Cells Kinase Beta (IkBKb), a subunit of IKK, plays a crucial role in intracellular signaling, especially in inflammation and immune responses. In addition, the binding of TRAF6 to IkBKB is an important link in the immune response, which is not only involved in the initiation of inflammatory signals, but also in its regulation, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human IkBKb and recombinant mouse TRAF6. Briefly, IkBKb was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to TRAF6-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IkBKb pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human IkBKb and recombinant mouse TRAF6 was shown in Figure 1, the EC50 for this effect is 0.11 ug/mL.)