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Standard Curve (Sample)

Human sema domain, immunoglobulin domain (Ig) , transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D ELISA Kit | SEMA4D elisa kit

Human soluble Cluster of differentiation 100, sCD100 ELISA Kit

Gene Names
SEMA4D; CD100; SEMAJ; coll-4; C9orf164; M-sema-G
Reactivity
Human
Synonyms
sema domain, immunoglobulin domain (Ig) , transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D; N/A; Human soluble Cluster of differentiation 100, sCD100 ELISA Kit; Human soluble Cluster of differentiation 100 (sCD100) ELISA kit; XXyac-YM68G7.1; C9orf164; CD100; FLJ33485; FLJ34282; FLJ39737; FLJ46484; M-sema-G; MGC169138; MGC169141; SEMAJ; coll-4; OTTHUMP00000021622; OTTHUMP00000208950; OTTHUMP00000208951; OTTHUMP00000208957; se; sema domain; immunoglobulin domain (Ig); transmembrane domain (TM) and short cytoplasmic domain; (semaphorin) 4D; SEMA4D elisa kit
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For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human sCD100. No significant cross-reactivity or interference between human sCD100 and analogues was observed.
Sequence Length
738
Samples
Serum, plasma, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
125 pg/ml-8000 pg/ml
Sensitivity
< 31.25 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Standard Curve (Sample)

Related Product Information for SEMA4D elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for sCD100 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any sCD100 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for sCD100 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of sCD100 bound in the initial step. The color development is stopped and the intensity of the color is measured.
References
Increased soluble CD72 in systemic lupus erythematosus is in association with disease activity and lupus nephritis. Zahava Vadasz, Yair Goldeberg, Katty Halasz, Itzhak Rosner, Guido Valesini, Fabrizio Conti, Carlo Perricone, Zev Sthoeger, Shira Rosenberg Bezalel, Athanasios G. Tzioufas, Nancy Agmon Levin, Yehuda Shoenfeld and Elias Toubi (2016)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
96,150 Da
NCBI Official Full Name
semaphorin-4D isoform 2
NCBI Official Synonym Full Names
sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
NCBI Official Symbol
SEMA4D
NCBI Official Synonym Symbols
CD100; SEMAJ; coll-4; C9orf164; M-sema-G
NCBI Protein Information
semaphorin-4D; A8; GR3; BB18; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, 4D
UniProt Protein Name
Semaphorin-4D
UniProt Gene Name
SEMA4D
UniProt Synonym Gene Names
C9orf164; CD100; SEMAJ
UniProt Entry Name
SEM4D_HUMAN

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Product Notes

The Human SEMA4D sema4d (Catalog #AAA15737) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA15737 ELISA Kit recognizes Human SEMA4D. It is sometimes possible for the material contained within the vial of "sema domain, immunoglobulin domain (Ig) , transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.