Human serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor) , member 2 ELISA Kit | SERPINF2 elisa kit

Human alpha2-Antiplasmin, alpha2-AP ELISA Kit

Cat. Num. AAA15039

• Gene Names: SERPINF2; AAP; API; PLI; A2AP; ALPHA-2-PI
• Reactivity: Human
• Synonyms: serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor) , member 2; N/A; Human alpha2-Antiplasmin, alpha2-AP ELISA Kit; Human alpha2-Antiplasmin; alpha2-AP ELISA Kit; A2AP; AAP; ALPHA-2-PI; API; PLI; OTTHUMP00000115656; alpha-2-antiplasmin; alpha-2-plasmin inhibitor; serine (or cysteine) proteinase inhibitor; clade F (alpha-2 antiplasmin; pigment epithelium derived; serpin peptidase inhibitor; pigment epithelium derived factor); member 2; SERPINF2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human alpha2 -AP. No significant cross-reactivity or interference between human alpha2 -AP and analogues was observed.
• Sequence Length: 491
• Samples: Serum, plasma
• Assay Type: Sandwich
• Detection Range: 15.6 pg/ml -1000 pg/ml.
• Sensitivity: The minimum detectable dose of human alpha2 -AP is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Standard Curve (Sample)

Related Product Information for SERPINF2 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for alpha2-AP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any alpha2-AP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for alpha2-AP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of alpha2-AP bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 115583663
• NCBI GeneID: 5345
• NCBI Accession #: NP_000925.2
• NCBI GenBank Nucleotide #: NM_000934.3
• UniProt Accession #: P08697; Q8N5U7; Q9UCG2; Q9UCG3; B4E1B7
• Molecular Weight: 54,566 Da
• NCBI Official Full Name: alpha-2-antiplasmin isoform a
• NCBI Official Synonym Full Names: serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2
• NCBI Official Symbol: SERPINF2
• NCBI Official Synonym Symbols: AAP; API; PLI; A2AP; ALPHA-2-PI
• NCBI Protein Information: alpha-2-antiplasmin; serpin F2; alpha-2-AP; alpha-2-plasmin inhibitor; serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2
• UniProt Protein Name: Alpha-2-antiplasmin
• UniProt Gene Name: SERPINF2
• UniProt Synonym Gene Names: AAP; PLI; Alpha-2-AP; Alpha-2-PI
• UniProt Entry Name: A2AP_HUMAN