SDS-PAGE
SET recombinant protein
SET, 1-290aa, Human, His tag, E Coli
Gene Names
SET; 2PP2A; IGAAD; TAF-I; I2PP2A; IPP2A2; PHAPII; TAF-IBETA
Applications
SDS-Page
Purity
> 90% by SDS-PAGE
Synonyms
SET; N/A; SET, 1-290aa, Human, His tag, E Coli; Protein SET isoform 1; 2PP2A; I2PP2A; IGAAD; IPP2A2; PHAPII; TAF-I; TAF-IBETA; SET recombinant protein
Host
E Coli
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT.
Concentration
0.5 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAPKRQS PLPPQKKKPR PPPALGPEET SASAGLPKKG EKEQQEAIEH IDEVQNEIDR LNEQASEEIL KVEQKYNKLR QPFFQKRSEL IAKIPNFWVT TFVNHPQVSA LLGEEDEEAL HYLTRVEVTE FEDIKSGYRI DFYFDENPYF ENKVLSKEFH LNESGDPSSK STEIKWKSGK DLTKRSSQTQ NKASRKRQHE EPESFFTWFT DHSDAGADEL GEVIKDDIWP NPLQYYLVPD MDDEEGEGEE DDDDDEEEEG LEDIDEEGDE DEGEEDEDDD EGEEGEEDEG EDD
Sequence Length
290
Applicable Applications for SET recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-PAGE
SDS-PAGE
Related Product Information for SET recombinant protein
SET inhibits acetylation of nucleosomes, especially histone H4, by histone acetylases (HAT). This inhibition is most likely accomplished by masking histone lysines from being acetylated, and the consequence is to silence HAT-dependent transcription. The protein is part of a complex localized to the endoplasmic reticulum but is found in the nucleus and inhibits apoptosis following attack by cytotoxic T lymphocytes. This protein can also enhance DNA replication of the adenovirus genome. Several transcript variants encoding different isoforms have been found for this gene. Recombinant human SET protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for SET recombinant protein
References
Karetsou Z, Emmanouilidou A, et al. (2009). BMC Biochem. 10:10. ; Li M, Makkinje A, et al. (1996). J Biol Chem. 271 (19):11059-62.;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35.9 kDa (313aa), confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)
NCBI Official Full Name
protein SET isoform 1
NCBI Official Synonym Full Names
SET nuclear proto-oncogene
NCBI Official Symbol
SET
NCBI Official Synonym Symbols
2PP2A; IGAAD; TAF-I; I2PP2A; IPP2A2; PHAPII; TAF-IBETA
NCBI Protein Information
protein SET
UniProt Protein Name
Protein SET
UniProt Gene Name
SET
UniProt Synonym Gene Names
IGAAD; I-2PP2A; TAF-I
Similar Products
Product Notes
The SET set (Catalog #AAA48843) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's SET can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the SET set for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSMAPKRQS PLPPQKKKPR PPPALGPEET SASAGLPKKG EKEQQEAIEH IDEVQNEIDR LNEQASEEIL KVEQKYNKLR QPFFQKRSEL IAKIPNFWVT TFVNHPQVSA LLGEEDEEAL HYLTRVEVTE FEDIKSGYRI DFYFDENPYF ENKVLSKEFH LNESGDPSSK STEIKWKSGK DLTKRSSQTQ NKASRKRQHE EPESFFTWFT DHSDAGADEL GEVIKDDIWP NPLQYYLVPD MDDEEGEGEE DDDDDEEEEG LEDIDEEGDE DEGEEDEDDD EGEEGEEDEG EDD. It is sometimes possible for the material contained within the vial of "SET, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.