Purity
(The purity of LRRK2 (I2020T) was determined to be >70% by densitometry, approx. MW 210 kDa.)
LRRK2 active protein
LRRK2 Mutant (I2020T), Active
Gene Names
LRRK2; PARK8; RIPK7; ROCO2; AURA17; DARDARIN
Purity
>70%
Synonyms
LRRK2; N/A; LRRK2 Mutant (I2020T), Active; LRRK2 active protein
Host
Sf9 cells
Purity/Purification
>70%
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Concentration
0.05 ug/ul (varies by lot)
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70°C.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity
(The purity of LRRK2 (I2020T) was determined to be >70% by densitometry, approx. MW 210 kDa.)
Purity
(The purity of LRRK2 (I2020T) was determined to be >70% by densitometry, approx. MW 210 kDa.)
Specific Activity
(The specific activity of LRRK2 (I2020T) was determined to be 7 nmol/min/mg as per activity assay protocol.)
Specific Activity
(The specific activity of LRRK2 (I2020T) was determined to be 7 nmol/min/mg as per activity assay protocol.)
Related Product Information for LRRK2 active protein
Description: Recombinant human LRRK2 (I2020T) (968-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The LRRK2 gene accession number is NM_198578.
Scientific Background: LRRK2 or leucine-rich repeat kinase is a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain. Mutations in LRRK2 are the most frequent known cause of autosomal dominant and idiopathic Parkinson's disease with prevalent mutations being found within the GTPase and kinase domains (1). LRRK2 cooperates with MET to promote efficient tumor cell growth and survival in various cancers. Down-regulation of LRRK2 in cultured tumor cells compromises MET activation and selectively reduces downstream MET signaling to mTOR and STAT3 (2).
Scientific Background: LRRK2 or leucine-rich repeat kinase is a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain. Mutations in LRRK2 are the most frequent known cause of autosomal dominant and idiopathic Parkinson's disease with prevalent mutations being found within the GTPase and kinase domains (1). LRRK2 cooperates with MET to promote efficient tumor cell growth and survival in various cancers. Down-regulation of LRRK2 in cultured tumor cells compromises MET activation and selectively reduces downstream MET signaling to mTOR and STAT3 (2).
Product Categories/Family for LRRK2 active protein
References
1. Yao C, LRRK2-mediated neurodegeneration and dysfunction of dopaminergic neurons in a Caenorhabditis elegans model of Parkinson's disease. Neurobiol Dis. 2010 Oct;40 (1):73-81. 2. Looyenga BD, Chromosomal amplification of leucine-rich repeat kinase-2 (LRRK2) is required for oncogenic MET signaling in papillary renal and thyroid carcinomas. Proc Natl Acad Sci U S A. 2011 Jan 25;108 (4):1439-44.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
Homo sapiens leucine rich repeat kinase 2 (LRRK2), mRNA
NCBI Official Synonym Full Names
leucine rich repeat kinase 2
NCBI Official Symbol
LRRK2
NCBI Official Synonym Symbols
PARK8; RIPK7; ROCO2; AURA17; DARDARIN
NCBI Protein Information
leucine-rich repeat serine/threonine-protein kinase 2
UniProt Protein Name
Leucine-rich repeat serine/threonine-protein kinase 2
UniProt Gene Name
LRRK2
UniProt Synonym Gene Names
PARK8
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Product Notes
The LRRK2 lrrk2 (Catalog #AAA14255) is an Active Protein produced from Sf9 cells and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "LRRK2, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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