Purity Info
(The purity of PIP5K1C was detrermined to be >70% by densitometry approx. MW 120 kDa.)
PIP5K1C active protein
PIP5K1C, Active
Gene Names
PIP5K1C; LCCS3; PIP5Kgamma; PIP5K-GAMMA; PIP5K1-gamma
Purity
>80%
Synonyms
PIP5K1C; N/A; PIP5K1C, Active; KIAA0589; LCCS3; PlP5K-GAMMA; PlP5Kgamma; PlPKlg_v4; PIP5K1C active protein
Host
Sf9 cells
Purity/Purification
>80%
Form/Format
Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM, DTT, 0.1mM PMSF, and 25% glycerol.
Concentration
0.1 mg/ml (varies by lot)
Host Note
Recombinant full length human protein expressed in Sf9 cells.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Ships on dry ice. Store product at -70 degree C.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Purity Info
(The purity of PIP5K1C was detrermined to be >70% by densitometry approx. MW 120 kDa.)
Purity Info
(The purity of PIP5K1C was detrermined to be >70% by densitometry approx. MW 120 kDa.)
Specific Activity
(The specific activity of PIP5K1C was determined to be 6,927 nmol/min/mg as per activity assay protocol.)
Specific Activity
(The specific activity of PIP5K1C was determined to be 6,927 nmol/min/mg as per activity assay protocol.)
Related Product Information for PIP5K1C active protein
Description: Recombinant full-length human PIP5K1C was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: PIP5K1C or phosphatidylinositol-4-phosphate 5-kinase 1 gamma phosphorylates phosphatidylinositol-4-phosphate to form phosphatidylinositol 4,5-bisphosphate. PIP5K1C is found at synapses and has been found to play roles in endocytosis and cell migration. PIP5K1C directly associates with beta-catenin and increases beta-catenin activity downstream of growth factor stimulation (1). PIP5K1C expression and kinase activity enhance beta-catenin phosphorylation on residues that promote nuclear importation and transcriptional activity. PIP5K1C deficiency impairs neutrophil recruitment as a result of defect in adhesion. PIP5K1C regulated the adhesion through facilitating RhoA GTPase and integrin activation by chemoattractants (2).
Scientific Background: PIP5K1C or phosphatidylinositol-4-phosphate 5-kinase 1 gamma phosphorylates phosphatidylinositol-4-phosphate to form phosphatidylinositol 4,5-bisphosphate. PIP5K1C is found at synapses and has been found to play roles in endocytosis and cell migration. PIP5K1C directly associates with beta-catenin and increases beta-catenin activity downstream of growth factor stimulation (1). PIP5K1C expression and kinase activity enhance beta-catenin phosphorylation on residues that promote nuclear importation and transcriptional activity. PIP5K1C deficiency impairs neutrophil recruitment as a result of defect in adhesion. PIP5K1C regulated the adhesion through facilitating RhoA GTPase and integrin activation by chemoattractants (2).
Product Categories/Family for PIP5K1C active protein
References
1. Schramp M. et al: PIPKIgamma regulates beta-catenin transcriptional activity downstream of growth factor receptor signaling. Cancer Res. 2011 Feb 15;71 (4):1282-91. 2. Xu W, Integrin-induced PIP5K1C kinase polarization regulates neutrophil polarization, directionality, and in vivo infiltration. Immunity. 2010 Sep 24;33 (3):340-50.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
Homo sapiens phosphatidylinositol-4-phosphate 5-kinase type 1 gamma (PIP5K1C), transcript variant 2, mRNA
NCBI Official Synonym Full Names
phosphatidylinositol-4-phosphate 5-kinase type 1 gamma
NCBI Official Symbol
PIP5K1C
NCBI Official Synonym Symbols
LCCS3; PIP5Kgamma; PIP5K-GAMMA; PIP5K1-gamma
NCBI Protein Information
phosphatidylinositol 4-phosphate 5-kinase type-1 gamma
UniProt Protein Name
Phosphatidylinositol 4-phosphate 5-kinase type-1 gamma
UniProt Gene Name
PIP5K1C
UniProt Synonym Gene Names
KIAA0589; PIP5K1-gamma; PtdIns(4)P-5-kinase 1 gamma; PIP5KIgamma
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Product Notes
The PIP5K1C pip5k1c (Catalog #AAA73045) is an Active Protein produced from Sf9 cells and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "PIP5K1C, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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