Human Soluble Leukocyte antigen G ELISA Kit | sHLA G elisa kit

Human Soluble Leukocyte antigen G ELISA Kit

Cat. Num. AAA87113

• Gene Names: SMAD1; BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1
• Reactivity: Human
• Synonyms: Soluble Leukocyte antigen G; N/A; Human Soluble Leukocyte antigen G ELISA Kit; sHLA G elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of sHLA-G. No significant cross-reactivity or interference between sHLA-G and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between sHLA-G and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for sHLA G elisa kit

Principle of the Assay: sHLA-G ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-sHLA-G antibody and an sHLA-G-HRP conjugate. The assay sample and buffer are incubated together with sHLA-G-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the sHLA-G concentration since sHLA-G from samples and sHLA-G-HRP conjugate compete for the anti-sHLA-G antibody binding site. Since the number of sites is limited, as more sites are occupied by sHLA-G from the sample, fewer sites are left to bind sHLA-G-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sHLA-G concentration in each sample is interpolated from this standard curve.

Intended Uses: This sHLA-G ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human sHLA-G. This ELISA kit for research use only, not for therapeutic or test applications!

Product Categories/Family for sHLA G elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 13633915
• NCBI GeneID: 4086
• Molecular Weight: 52,260 Da
• NCBI Official Full Name: Mothers against decapentaplegic homolog 1
• NCBI Official Synonym Full Names: SMAD family member 1
• NCBI Official Symbol: SMAD1
• NCBI Official Synonym Symbols: BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1
• NCBI Protein Information: mothers against decapentaplegic homolog 1; MAD homolog 1; Mad-related protein 1; TGF-beta signaling protein 1; mothers against DPP homolog 1; SMAD, mothers against DPP homolog 1; MAD, mothers against decapentaplegic homolog 1; transforming growth factor-beta signaling protein 1; transforming growth factor-beta-signaling protein 1
• UniProt Protein Name: Mothers against decapentaplegic homolog 1
• UniProt Gene Name: SMAD1
• UniProt Synonym Gene Names: BSP1; MADH1; MADR1; MAD homolog 1; Mothers against DPP homolog 1; SMAD 1; Smad1; hSMAD1; BSP-1
• UniProt Entry Name: SMAD1_HUMAN