Application Data
(The purity of SIRT6 was determined to be >90% by densitometry. Approx. MW 39kDa.)
SIRT6 recombinant protein
SIRT6, Active
Gene Names
SIRT6; SIR2L6
Purity
>90%
Synonyms
SIRT6; N/A; SIRT6, Active; SIRT6 recombinant protein
Host
Sf9 cells
Purity/Purification
>90%
Form/Format
50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
Concentration
0.1ug/ul (varies by lot)
Sequence Length
1662
Specific Activity
120 RLU/min/ng
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70 degree C.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature.
For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Application Data
(The purity of SIRT6 was determined to be >90% by densitometry. Approx. MW 39kDa.)
Application Data
(The purity of SIRT6 was determined to be >90% by densitometry. Approx. MW 39kDa.)
Application Data
(The specific activity of SIRT6 was determined to be 120 RLU/min/ng as per activity assay protocol)
Application Data
(The specific activity of SIRT6 was determined to be 120 RLU/min/ng as per activity assay protocol)
Related Product Information for SIRT6 recombinant protein
Description: Recombinant human SIRT6 (23-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag.
Scientific Background: SIRT6 is a member of the sirtuin family of proteins which are homologs to the yeast Sir2 protein. Sirtuin family contain a sirtuin core domain and are grouped into four classes with SIRT6 being a member of class IV. Human SIRT6 protein is a NAD (+)-dependent histone H3 lysine-9 deacetylase that modulates telomeric chromatin (1). SIRT6 associates specifically with telomeres and SIRT6 depletion leads to telomere dysfunction with end-to-end chromosomal fusions and premature cellular senescence. SIRT6 -/- mouse cells show that SIRT6 promotes resistance to DNA damage and suppresses genomic instability in association with a role in base excision repair (2).
Scientific Background: SIRT6 is a member of the sirtuin family of proteins which are homologs to the yeast Sir2 protein. Sirtuin family contain a sirtuin core domain and are grouped into four classes with SIRT6 being a member of class IV. Human SIRT6 protein is a NAD (+)-dependent histone H3 lysine-9 deacetylase that modulates telomeric chromatin (1). SIRT6 associates specifically with telomeres and SIRT6 depletion leads to telomere dysfunction with end-to-end chromosomal fusions and premature cellular senescence. SIRT6 -/- mouse cells show that SIRT6 promotes resistance to DNA damage and suppresses genomic instability in association with a role in base excision repair (2).
Product Categories/Family for SIRT6 recombinant protein
References
1. Michishita, E. et al: SIRT6 is a histone H3 lysine 9 deacetylase that modulates telomeric chromatin. Nature 452: 492-496, 2008. 2. Mostoslavsky, R. et al: Genomic instability and aging-like phenotype in the absence of mammalian SIRT6. Cell 124: 315-329, 2006
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
39 kDa
NCBI Official Full Name
Homo sapiens sirtuin 6 (SIRT6), transcript variant 1, mRNA
NCBI Official Synonym Full Names
sirtuin 6
NCBI Official Symbol
SIRT6
NCBI Official Synonym Symbols
SIR2L6
NCBI Protein Information
NAD-dependent protein deacetylase sirtuin-6
UniProt Protein Name
NAD-dependent protein deacetylase sirtuin-6
UniProt Gene Name
SIRT6
UniProt Synonym Gene Names
SIR2L6
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Product Notes
The SIRT6 sirt6 (Catalog #AAA73142) is a Recombinant Protein produced from Sf9 cells and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "SIRT6, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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