SDS-PAGE
Sirtuin 3 (SIRT3) Recombinant Protein | SIRT3 recombinant protein
Recombinant Sirtuin 3 (SIRT3)
Applications
Immunoprecipitation, ELISA, Western Blot, SDS-Page
Purity
>95%
Synonyms
Sirtuin 3 (SIRT3); N/A; Recombinant Sirtuin 3 (SIRT3); SIRT3 recombinant protein
Host
E Coli
Purity/Purification
>95%
Form/Format
Supplied as lyophilized form in 100mM NaHCO3, 500mM NaCl, pH8.3, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
Sequence
GISTPS GIPDFRSPGS GLYSNLQQYD IPYPEAIFEL GFFFHNPKPF FMLAKELYPG HYRPNVTHYF LRLLHDKELL LRLYTQNIDG LERASGIPAS KLVEAHGTFV TATCTVCRRS FPGEDIWADV MADRVPRCPV CTGVVKPDIV FFGEQLPARF LLHMADFALA DLLLILGTSL EVEPFASLSE AVQKSVPRLL INRDLVGPFV LSPRRKDVVQ LGDVVHGVER LVDLLGWTQE LLDLMQRERG KLDGQDR
Applicable Applications for SIRT3 recombinant protein
IP (Immunoprecipitation), ELISA, WB (Western Blot), SDS-PAGE
Residues
Gly5~Arg257
Tags
Two N-terminal Tags, His-tag and T7-tag
Subcellular Location
Cell membrane; Single-pass type II membrane protein.
Endotoxin Level
<1.0EU per 1 ug (determined by the LAL method).
Predicted isoelectric point
6.4
Predicted Molecular Mass
32.3kDa
Accurate Molecular Mass
16&29kDa as determined by SDS-PAGE reducing conditions.
Reconstitution
Reconstitute in ddH2O
Preparation and Storage
Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
SDS-PAGE
SDS-PAGE
Sequence
Sequence
References
1. Vassilopoulos A., et al. (2014) Antioxid. Redox Signal. 21:551-564.
2. Finley L.W., et al. (2011) PLoS ONE 6:E23295-E23295.
3. Ahn B.-H., et al. (2008) Proc. Natl. Acad. Sci. U.S.A. 105:14447-14452.
4. Hallows W.C., et al. (2006) Proc. Natl. Acad. Sci. U.S.A. 103:10230-10235.
2. Finley L.W., et al. (2011) PLoS ONE 6:E23295-E23295.
3. Ahn B.-H., et al. (2008) Proc. Natl. Acad. Sci. U.S.A. 105:14447-14452.
4. Hallows W.C., et al. (2006) Proc. Natl. Acad. Sci. U.S.A. 103:10230-10235.
NCBI and Uniprot Product Information
UniProt Accession #
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Product Notes
The SIRT3 (Catalog #AAA140913) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Sirtuin 3 (SIRT3) can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ELISA, WB (Western Blot), SDS-PAGE. Researchers should empirically determine the suitability of the SIRT3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: GISTPS GIPDFRSPGS GLYSNLQQYD IPYPEAIFEL GFFFHNPKPF FMLAKELYPG HYRPNVTHYF LRLLHDKELL LRLYTQNIDG LERASGIPAS KLVEAHGTFV TATCTVCRRS FPGEDIWADV MADRVPRCPV CTGVVKPDIV FFGEQLPARF LLHMADFALA DLLLILGTSL EVEPFASLSE AVQKSVPRLL INRDLVGPFV LSPRRKDVVQ LGDVVHGVER LVDLLGWTQE LLDLMQRERG KLDGQDR. It is sometimes possible for the material contained within the vial of "Sirtuin 3 (SIRT3), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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