SDS-PAGE
Sphingomyelin Phosphodiesterase 3 Recombinant Protein | SMPD3 recombinant protein
Recombinant Sphingomyelin Phosphodiesterase 3 (SMPD3)
Gene Names
Smpd3; fro; nSMase2; AI427456; AW537966; 4631433G07Rik
Reactivity
Mus musculus (Mouse)
Applications
Western Blot, SDS-Page
Purity
> 95%
Synonyms
Sphingomyelin Phosphodiesterase 3; N/A; Recombinant Sphingomyelin Phosphodiesterase 3 (SMPD3); NSMASE2; Neutral Sphingomyelinase II; SMPD3 recombinant protein
Host
E.coli
Reactivity
Mus musculus (Mouse)
Purity/Purification
> 95%
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
Concentration
Original Concentration: 400ug/mL (varies by lot)
Applicable Applications for SMPD3 recombinant protein
WB (Western Blot), SDS-PAGE, Immunogen, Positive Control
Source
Prokaryotic expression
Residues
Asp415~Glu652
Tags
N-terminal His Tag
Subcellular Location
Membrane
Traits
Freeze-dried powder
Predicted isoelectric point
4.5
Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
SDS-PAGE
SDS-PAGE
Sequence
Sequence
Product Categories/Family for SMPD3 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Predicted Molecular Mass: 30.1kDa
Accurate Molecular Mass: 33kDa as determined by SDS-PAGE reducing conditions.
Accurate Molecular Mass: 33kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
sphingomyelin phosphodiesterase 3
NCBI Official Synonym Full Names
sphingomyelin phosphodiesterase 3, neutral
NCBI Official Symbol
Smpd3
NCBI Official Synonym Symbols
fro; nSMase2; AI427456; AW537966; 4631433G07Rik
NCBI Protein Information
sphingomyelin phosphodiesterase 3
UniProt Protein Name
Sphingomyelin phosphodiesterase 3
UniProt Gene Name
Smpd3
UniProt Synonym Gene Names
nSMase-2; nSMase2
UniProt Entry Name
NSMA2_MOUSE
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Product Notes
The SMPD3 smpd3 (Catalog #AAA150290) is a Recombinant Protein produced from E.coli and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Sphingomyelin Phosphodiesterase 3 (SMPD3) reacts with Mus musculus (Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's Sphingomyelin Phosphodiesterase 3 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), SDS-PAGE, Immunogen, Positive Control. Researchers should empirically determine the suitability of the SMPD3 smpd3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Sphingomyelin Phosphodiesterase 3, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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