Human sperm associated antigen 5 ELISA Kit | SPAG5 elisa kit
Human Sperm-associated antigen 5, SPAG5 ELISA Kit
Gene Names
SPAG5; MAP126; DEEPEST; hMAP126
Reactivity
Human
Synonyms
sperm associated antigen 5; N/A; Human Sperm-associated antigen 5, SPAG5 ELISA Kit; Human Sperm-associated antigen 5 (SPAG5) ELISA kit; DEEPEST; MAP126; hMAP126; astrin; mitotic spindle associated protein p126; mitotic spindle coiled-coil related protein; SPAG5 elisa kit
Reactivity
Human
Specificity
No significant cross-reactivity or interference between Human SPAG5 and analogues was observed.
Samples
Serum, Plasma, Tissue Homogenate, Feces and Urine
Assay Type
Sandwich
Detection Range
0.625 ng/ml - 20 ng/ml.
Sensitivity
0.1 ng/ml.
Intended Uses
This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated SPAG5 concentrations in Human serum, plasma and other body fluids. Using Purified Human SPAG5 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SPAG5 and SPAG5 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SPAG5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Intra-assay Precision
Intra-assay CV (%) is less than 15%.
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
Preparation and Storage
Store all reagents at 2-8 degree C
Related Product Information for SPAG5 elisa kit
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated SPAG5 concentrations in Human serum, plasma and other body fluids. Using Purified Human SPAG5 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add SPAG5 and SPAG5 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SPAG5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
134,422 Da
NCBI Official Full Name
sperm-associated antigen 5
NCBI Official Synonym Full Names
sperm associated antigen 5
NCBI Official Symbol
SPAG5
NCBI Official Synonym Symbols
MAP126; DEEPEST; hMAP126
NCBI Protein Information
sperm-associated antigen 5; astrin; mitotic spindle associated protein p126; mitotic spindle-associated protein p126; mitotic spindle coiled-coil related protein
UniProt Protein Name
Sperm-associated antigen 5
UniProt Gene Name
SPAG5
UniProt Synonym Gene Names
MAP126
UniProt Entry Name
SPAG5_HUMAN
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Product Notes
The Human SPAG5 spag5 (Catalog #AAA299947) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA299947 ELISA Kit recognizes Human SPAG5. It is sometimes possible for the material contained within the vial of "sperm associated antigen 5, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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