Standard Curve (Sample)
Mouse OPN ELISA Kit | SPP1 elisa kit
Mouse OPN PicoKine ELISA Kit
Gene Names
Spp1; OP; 2AR; Bsp; Eta; Opn; Ric; BNSP; BSPI; Opnl; Apl-1; ETA-1; Spp-1
Reactivity
Mouse
Synonyms
OPN; N/A; Mouse OPN PicoKine ELISA Kit; Osteopontin; secreted phosphoprotein 1; BNSP; Bone sialoprotein 1; Bone sialoprotein I; BSPI; Early T lymphocyte activation 1; ETA 1; MGC110940; Nephropontin; OSTP_HUMAN; Secreted phosphoprotein 1; Secreted phosphoprotein 1 (osteopontin bone sialoprotein I early T lymphocyte activation 1); Secreted phosphoprotein 1 (osteopontin bone sialoprotein); SPP-1; SPP1; Urinary stone protein; Uropontin; SPP1 elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant Mouse Spp1
Sequence Length
294
Samples
Cell culture supernatants, serum, plasma (heparin, EDTA) and urine
Detection Range
156 pg/ml - 10,000 pg/ml
Sensitivity
<10 pg/ml
Intra-assay Precision
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for SPP1 elisa kit
Background: Osteopontin (OPN) also called urinary stone protein, secreted phosphoprotein 1 (SPP1), bone sialoprotein, and early T lymphocyte activation 1 (ETA1). Osteopontin is a phosphorylated glycoprotein secreted to the mineralizing extrOPNllular matrix by osteoblasts during bone development. It is believed to facilitate the attachment of osteoblasts and osteoclasts to the extrOPNllular matrix, allowing them to perform their respective functions during osteogenesis. Osteopontin is presumably involved in stone formation as stone matrix. The deduced protein sequence reveals a 317-amino acid protein (34,982 Da) containing a 16-amino acid hydrophobic signal sequence and a 33,352-Da protein destines to undergo extensive post-translational modifications before being secreted from the cell. The gene is located on human chromosome 4. The standard product used in this kit is recombinant mouse OPN, consisting of 284 amino acid sequence with the molecular mass of 31.5KDa. As a result of glycosylation, the molecular mass is 65KDa.
Principle of the Assay: The Picokine™ Mouse Spp1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Mouse Spp1 with a 96-well strip plate that is pre-coated with antibody specific for Spp1. The detection antibody is a biotinylated antibody specific for Spp1. The capture antibody is monoclonal antibody from rat and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Mouse Spp1 with immunogen: Expression system for standard: NS0; Immunogen sequence: L17-N294. The kit is analytically validated with ready-to-use reagents. To measure Mouse Spp1, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Mouse Spp1 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Spp1 in the sample.
Principle of the Assay: The Picokine™ Mouse Spp1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Mouse Spp1 with a 96-well strip plate that is pre-coated with antibody specific for Spp1. The detection antibody is a biotinylated antibody specific for Spp1. The capture antibody is monoclonal antibody from rat and the detection antibody is polyclonal antibody from goat. The kit contains recombinant Mouse Spp1 with immunogen: Expression system for standard: NS0; Immunogen sequence: L17-N294. The kit is analytically validated with ready-to-use reagents. To measure Mouse Spp1, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Mouse Spp1 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Spp1 in the sample.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
32,429 Da
NCBI Official Full Name
Bone marrow macrophage cDNA, RIKEN full-length enriched library, clone:I830015P03 product:secreted phosphoprotein 1, full insert sequence
NCBI Official Synonym Full Names
secreted phosphoprotein 1
NCBI Official Symbol
Spp1
NCBI Official Synonym Symbols
OP; 2AR; Bsp; Eta; Opn; Ric; BNSP; BSPI; Opnl; Apl-1; ETA-1; Spp-1
NCBI Protein Information
osteopontin; minopontin; bone sialoprotein 1; osteopontin-like protein; 44 kDa bone phosphoprotein; early T-lymphocyte activation 1 protein; calcium oxalate crystal growth inhibitor protein
UniProt Protein Name
Putative uncharacterized protein
UniProt Gene Name
Spp1
UniProt Entry Name
Q3UBR1_MOUSE
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Product Notes
The Mouse SPP1 spp1 (Catalog #AAA45229) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA45229 ELISA Kit recognizes Mouse SPP1. It is sometimes possible for the material contained within the vial of "OPN, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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