Standard Curve (Sample)
(Fig.1. Human Spectrin beta chain, brain 3 (SPTBN4) Standard Curve.)
Human Spectrin beta Chain, Brain 3 (SPTBN4) ELISA Kit | SPTBN3 elisa kit
Human Spectrin beta Chain, Brain 3 (SPTBN4) ELISA Kit
Gene Names
SPTBN4; QV; CMND; SPNB4; NEDHND; SPTBN3
Reactivity
Human
Synonyms
Spectrin beta Chain, Brain 3 (SPTBN4); N/A; Human Spectrin beta Chain, Brain 3 (SPTBN4) ELISA Kit; SPTBN4; KIAA1642; QV; SPNB4; betaIV spectrin; SPTBN3 elisa kit
Reactivity
Human
Specificity
Human Spectrin beta chain, brain 3 (SPTBN4) ELISA Kit has high sensitivity and excellent specificity for detection of Human SPTBN4. No significant cross-reactivity or interference between Human SPTBN4 and analogues was observed.
Sequence Length
678
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Spectrin beta chain, brain 3 (SPTBN4) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Spectrin beta chain, brain 3 (SPTBN4) Standard Curve.)
Related Product Information for SPTBN3 elisa kit
Description: This Human Spectrin beta chain, brain 3 (SPTBN4) ELISA Kit employs a two-site sandwich ELISA to quantitate SPTBN4 in samples. An antibody specific for SPTBN4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SPTBN4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPTBN4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPTBN4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Spectrin is an actin crosslinking and molecular scaffold protein that links the cell membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. It is composed of two antiparallel dimers of alpha- and beta- subunits. SPTbN4 is one member of a family of beta-spectrin genes. The encoded protein localizes to the nuclear matrix, PML nuclear bodies, and cytoplasmic vesicles. A highly similar gene in the mouse is required for localization of specific membrane proteins in polarized regions of neurons. Multiple transcript variants encoding different isoforms have been found for this gene. Abundantly expressed in brain and pancreatic islets.
Background: Spectrin is an actin crosslinking and molecular scaffold protein that links the cell membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. It is composed of two antiparallel dimers of alpha- and beta- subunits. SPTbN4 is one member of a family of beta-spectrin genes. The encoded protein localizes to the nuclear matrix, PML nuclear bodies, and cytoplasmic vesicles. A highly similar gene in the mouse is required for localization of specific membrane proteins in polarized regions of neurons. Multiple transcript variants encoding different isoforms have been found for this gene. Abundantly expressed in brain and pancreatic islets.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
spectrin beta chain, non-erythrocytic 4 isoform sigma5
NCBI Official Synonym Full Names
spectrin beta, non-erythrocytic 4
NCBI Official Symbol
SPTBN4
NCBI Official Synonym Symbols
QV; CMND; SPNB4; NEDHND; SPTBN3
NCBI Protein Information
spectrin beta chain, non-erythrocytic 4
UniProt Protein Name
Spectrin beta chain, non-erythrocytic 4
UniProt Gene Name
SPTBN4
UniProt Synonym Gene Names
KIAA1642; SPTBN3
UniProt Entry Name
SPTN4_HUMAN
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Product Notes
The Human SPTBN3 sptbn4 (Catalog #AAA330568) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330568 ELISA Kit recognizes Human SPTBN3. It is sometimes possible for the material contained within the vial of "Spectrin beta Chain, Brain 3 (SPTBN4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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