FCM/FACS (Flow Cytometry)
(Figure-3: Intracellular flow analysis of human Spectrin beta 3 in HeLa cells using 2 ug/10^6 cells of antibody (Clone: ABM5A16). Green represents isotype control; red represents anti-Spectrin beta 3 antibody. Goat anti-mouse PE conjugate was used as secondary antibody. (Cells were fixed with 4% paraformaldehyde for 10 min and washed with PBS by centrifuging at 1100 for 5 min followed by permeabilization for 20 min and washed again as mentioned above. Then cell were incubated with primary antibody for 45 min. and after washing the cells twice in PBS, incubated with conjugated secondary antibody for 30 min. Data acquisition was done after washing twice with PBS as mentioned above).)
Mouse anti-Human Spectrin beta 3 Monoclonal Antibody | anti-SPTBN2 antibody
Monoclonal Antibody to Spectrin beta 3 (Clone: ABM5A16)
Gene Names
SPTBN2; SCA5; SCAR14; GTRAP41
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Western Blot
Purity
PurifiedProtein G Chromatography
Synonyms
Spectrin beta 3, Antibody; Monoclonal Antibody to Spectrin beta 3 (Clone: ABM5A16); Spectrin beta chain; non-erythrocytic 2; Beta-III spectrin; Spinocerebellar ataxia 5 protein; anti-SPTBN2 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a, Kappa
Clone Number
ABM5A16
Purity/Purification
Purified
Protein G Chromatography
Protein G Chromatography
Form/Format
Purified
25 ug in 50 uL/100 ug in 200 uL in PBS containing 0.05%BSA and 0.05% sodium azide. Sodium azide is highly toxic.
25 ug in 50 uL/100 ug in 200 uL in PBS containing 0.05%BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Sequence Length
2390
Applicable Applications for anti-SPTBN2 antibody
FCM/FACS (Flow Cytometry), WB (Western Blot)
Immunogen
A partial length recombinant protein (a.a 1280-1450) of Spectrin beta 3 was used as the immunogen for this antibody.
Preparation and Storage
Store the antibody at 4°C; stable for 6 months.
For long-term storage; store at -20°C.
Avoid repeated freeze and thaw cycles.
For long-term storage; store at -20°C.
Avoid repeated freeze and thaw cycles.
FCM/FACS (Flow Cytometry)
(Figure-3: Intracellular flow analysis of human Spectrin beta 3 in HeLa cells using 2 ug/10^6 cells of antibody (Clone: ABM5A16). Green represents isotype control; red represents anti-Spectrin beta 3 antibody. Goat anti-mouse PE conjugate was used as secondary antibody. (Cells were fixed with 4% paraformaldehyde for 10 min and washed with PBS by centrifuging at 1100 for 5 min followed by permeabilization for 20 min and washed again as mentioned above. Then cell were incubated with primary antibody for 45 min. and after washing the cells twice in PBS, incubated with conjugated secondary antibody for 30 min. Data acquisition was done after washing twice with PBS as mentioned above).)
FCM/FACS (Flow Cytometry)
(Figure-3: Intracellular flow analysis of human Spectrin beta 3 in HeLa cells using 2 ug/10^6 cells of antibody (Clone: ABM5A16). Green represents isotype control; red represents anti-Spectrin beta 3 antibody. Goat anti-mouse PE conjugate was used as secondary antibody. (Cells were fixed with 4% paraformaldehyde for 10 min and washed with PBS by centrifuging at 1100 for 5 min followed by permeabilization for 20 min and washed again as mentioned above. Then cell were incubated with primary antibody for 45 min. and after washing the cells twice in PBS, incubated with conjugated secondary antibody for 30 min. Data acquisition was done after washing twice with PBS as mentioned above).)
FCM/FACS (Flow Cytometry)
(Figure-2: Intracellular flow analysis of human Spectrin beta 3 in A431 cells using 2 ug/10^6 cells of antibody (Clone: ABM5A16). Green represents isotype control; red represents anti-Spectrin beta 3 antibody. Goat anti-mouse PE conjugate was used as secondary antibody. (Cells were fixed with 4% paraformaldehyde for 10 min and washed with PBS by centrifuging at 1100 for 5 min followed by permeabilization for 20 min and washed again as mentioned above. Then cell were incubated with primary antibody for 45 min. and after washing the cells twice in PBS, incubated with conjugated secondary antibody for 30 min. Data acquisition was done after washing twice with PBS as mentioned above).)
FCM/FACS (Flow Cytometry)
(Figure-2: Intracellular flow analysis of human Spectrin beta 3 in A431 cells using 2 ug/10^6 cells of antibody (Clone: ABM5A16). Green represents isotype control; red represents anti-Spectrin beta 3 antibody. Goat anti-mouse PE conjugate was used as secondary antibody. (Cells were fixed with 4% paraformaldehyde for 10 min and washed with PBS by centrifuging at 1100 for 5 min followed by permeabilization for 20 min and washed again as mentioned above. Then cell were incubated with primary antibody for 45 min. and after washing the cells twice in PBS, incubated with conjugated secondary antibody for 30 min. Data acquisition was done after washing twice with PBS as mentioned above).)
WB (Western Blot)
(Figure-1: Western blot analysis of Spectrin beta 3. Anti-Spectrin beta 3 antibody (Clone: ABM5A16) was used at 0.1 ug/ml on h Kidney and h Brain lysates.)
WB (Western Blot)
(Figure-1: Western blot analysis of Spectrin beta 3. Anti-Spectrin beta 3 antibody (Clone: ABM5A16) was used at 0.1 ug/ml on h Kidney and h Brain lysates.)
Related Product Information for anti-SPTBN2 antibody
Beta-III spectrin is a protein encoded by the gene SPTBN2, spans 2390 amino acids and consists of an amino-terminal actin binding domain (ABD), a central region containing seventeen spectrin repeat domains, and a carboxy-terminal pleckstrin homology domain. It is critical for the correct development and maintenance of Purkinje cell dendritic structure. Beta-III spectrin is expressed predominantly in the brain and is enriched in cerebellar Purkinje cells. In addition, Beta-III spectrin participates in endomembrane trafficking through its interaction with the actin related protein, ARP1. The functional unit of Beta-III spectrin is considered to be a tetrameric complex composed of two Beta-spectrin subunits and two Alpha-II-spectrin subunits. Mutations in the gene encoding Beta-III spectrin give rise to spinocerebellar ataxia type 5, a neurodegenerative disease characterized by progressive thinning of the molecular layer, loss of Purkinje cells and increasing motor deficits.
Product Categories/Family for anti-SPTBN2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
268,309 Da
NCBI Official Full Name
spectrin beta chain, non-erythrocytic 2
NCBI Official Synonym Full Names
spectrin beta, non-erythrocytic 2
NCBI Official Symbol
SPTBN2
NCBI Official Synonym Symbols
SCA5; SCAR14; GTRAP41
NCBI Protein Information
spectrin beta chain, non-erythrocytic 2
UniProt Protein Name
Spectrin beta chain, non-erythrocytic 2
UniProt Gene Name
SPTBN2
UniProt Synonym Gene Names
KIAA0302; SCA5
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Product Notes
The SPTBN2 sptbn2 (Catalog #AAA78432) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to Spectrin beta 3 (Clone: ABM5A16) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Spectrin beta 3 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), WB (Western Blot). Researchers should empirically determine the suitability of the SPTBN2 sptbn2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Spectrin beta 3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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