Application Data
(Reverse transcription of a 330 bp fragment CCD1A RNA using RScript cDNA Synthesis Kit to carry this experiment. The PCR data shows that RScript cDNA Synthesis Kit have a stable and superior performance at higher temperatures.)
cDNA synthesis kit
RScript cDNA Synthesis Kit
Application Data
(Reverse transcription of a 330 bp fragment CCD1A RNA using RScript cDNA Synthesis Kit to carry this experiment. The PCR data shows that RScript cDNA Synthesis Kit have a stable and superior performance at higher temperatures.)
Application Data
(Reverse transcription of a 330 bp fragment CCD1A RNA using RScript cDNA Synthesis Kit to carry this experiment. The PCR data shows that RScript cDNA Synthesis Kit have a stable and superior performance at higher temperatures.)
Application Data
(Red: RScript reverse transcriptase #RT001-0050Green: Improm-II #A3802Blue: Superscript IV #18090010Figure 5. Performing RT-qPCR with RScript reverse transcriptase :At a temperature of 55 °C, RScript reverse transcriptase exceeds performance demonstrating better efficiency and higher cDNA yields. RScript reverse transcriptase shows a stable and superior performance at 55 ? compared to other brands. Therefore RScript reverse transcriptase does not encounter significant higher Ct values compared to Improm II and SuperScript IV due to their low amounts of input cDNA.*Promega Improm-II #A3802 is a registered trademark of Promega, Thermo Superscript-III #18080093 and Superscript-IV #18090010 are registered trademarks of Thermo Scientific. The trademark holders are not affiliated and do not endorse these products.)
Application Data
(Red: RScript reverse transcriptase #RT001-0050Green: Improm-II #A3802Blue: Superscript IV #18090010Figure 5. Performing RT-qPCR with RScript reverse transcriptase :At a temperature of 55 °C, RScript reverse transcriptase exceeds performance demonstrating better efficiency and higher cDNA yields. RScript reverse transcriptase shows a stable and superior performance at 55 ? compared to other brands. Therefore RScript reverse transcriptase does not encounter significant higher Ct values compared to Improm II and SuperScript IV due to their low amounts of input cDNA.*Promega Improm-II #A3802 is a registered trademark of Promega, Thermo Superscript-III #18080093 and Superscript-IV #18090010 are registered trademarks of Thermo Scientific. The trademark holders are not affiliated and do not endorse these products.)
Application Data
(Figure 4. Performance of One-Step RT-qPCR with RScript reverse transcriptase :To evaluate the RT performance for the COVID-19 fragments, the N1 gene with 100 copies per reaction was tested in the one-step RT-qPCR. Experiments proved to be feasible and effective (mean Ct : 34.88).* COVID-19 fragments were originally obtained from Twist Synthetic SARS-CoV-2 RNA Controls (# EPI_ISL_1662307).)
Application Data
(Figure 4. Performance of One-Step RT-qPCR with RScript reverse transcriptase :To evaluate the RT performance for the COVID-19 fragments, the N1 gene with 100 copies per reaction was tested in the one-step RT-qPCR. Experiments proved to be feasible and effective (mean Ct : 34.88).* COVID-19 fragments were originally obtained from Twist Synthetic SARS-CoV-2 RNA Controls (# EPI_ISL_1662307).)
Application Data
(Figure 3. Elongation of RNA templates up to 11 kb in length :RScript reverse transcriptase was used in a reaction with a range of human bladder cancer cell lines - 5637 (HTB-9) RNA. The resulting synthesized cDNA (8 kb ,11kb) was followed by PCR and visualized on a 2% agarose gel. The molecular weight marker used was DNA Ladder 1kb #SDL-1000R.)
Application Data
(Figure 3. Elongation of RNA templates up to 11 kb in length :RScript reverse transcriptase was used in a reaction with a range of human bladder cancer cell lines - 5637 (HTB-9) RNA. The resulting synthesized cDNA (8 kb ,11kb) was followed by PCR and visualized on a 2% agarose gel. The molecular weight marker used was DNA Ladder 1kb #SDL-1000R.)
Application Data
(A: RScript reverse transcriptase #RT001-0050/ RT001-0250B: Promega Improm II #A3802C: Thermo SuperScript III #18080093D: SuperScript IV #18090010 Figure 2. Enhancement of the thermostability:Reverse transcription of a 1 kb fragment ARHGAP29 RNA using RScript reverse transcriptase or competitor reverse transcriptases were used to carry this experiment. Regardless of the variations in temperatures (42 -60 °C) RScript reverse transcriptase shows a stable and superior performance at higher temperatures of up to 60 ? compared to other brands. The molecular weight marker used was DNA Ladder 1kb #SDL-1000R)
Application Data
(A: RScript reverse transcriptase #RT001-0050/ RT001-0250B: Promega Improm II #A3802C: Thermo SuperScript III #18080093D: SuperScript IV #18090010 Figure 2. Enhancement of the thermostability:Reverse transcription of a 1 kb fragment ARHGAP29 RNA using RScript reverse transcriptase or competitor reverse transcriptases were used to carry this experiment. Regardless of the variations in temperatures (42 -60 °C) RScript reverse transcriptase shows a stable and superior performance at higher temperatures of up to 60 ? compared to other brands. The molecular weight marker used was DNA Ladder 1kb #SDL-1000R)
Application Data
(Figure 1. Stability of RScript reverse transcriptase: The stability RT-qPCR data shows the performance of RScript reverse transcriptase is maintained without any significant alteration in performance at 37 °C after a period of 3 weeks (21 days), demonstrating its high resistance to temperature and time. The percentage of RScript reverse transcriptase activity was calculated by dividing values at each reaction temperature.)
Application Data
(Figure 1. Stability of RScript reverse transcriptase: The stability RT-qPCR data shows the performance of RScript reverse transcriptase is maintained without any significant alteration in performance at 37 °C after a period of 3 weeks (21 days), demonstrating its high resistance to temperature and time. The percentage of RScript reverse transcriptase activity was calculated by dividing values at each reaction temperature.)
RScript cDNA Synthesis Kit - Engineered innovatively and specifically for both Research and test applications for meeting all your cDNA synthesis needs and for overcoming the most challenging secondary RNA structures over a wide temperature range-is the latest addition to our reverse transcription product boutique. The RScript cDNA Synthesis Kit contains our next-generation, engineered recombinant M-MLV reverse transcriptase, with improved thermostability, processivity, robustness, optimal cDNA yields, proprietary site mutations for reduced RNase H activity, and extended half-life, is the most versatile reverse transcriptase in the world for not only simply meeting the routine cDNA synthesis requirements but also enabling superior performance for even the most challenging RNA samples at hand.
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Product Notes
The cDNA (Catalog #AAA13627) is a Synthesis Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "cDNA, Synthesis Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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