SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
T4 UvsY Recombinant Protein | T4 recombinant protein
T4 UvsY Protein
Purity
>80%
Synonyms
T4 UvsY; N/A; T4 UvsY Protein; Bacteriophage T4 DNA repair gene uvsY; recombination protein uvsY; T4 recombinant protein
Host
E Coli
Purity/Purification
>80%
Form/Format
Recombinant protein stored in 50mM Tris, 100mM KCl, 0.1mM EDTA, 50mM Arginine, 0.1% Triton X-100, 1mM DTT, 50% glycerol, pH8.0.
Concentration
2.0ug/ul (varies by lot)
Tag
HIS
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Aliquot product into smaller quantities after centrifugation and store at –70°C. Avoid repeated handling and multiple freeze/thaw cycles.
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Related Product Information for T4 recombinant protein
Description: Recombinant full-length T4 UvsY Protein from Escherichia virus T4 was expressed in E Coli using an N-terminal His-tag. The gene accession number is X04856.
Scientific Background: UvsY is a bacteriophage T4 recombination mediator protein (RMP) which promotes homologous recombination. UvsY exists in solution predominantly as a hexamer, and binds to ssDNA in this form. UvsY facilitates assembly of presynaptic filaments by loading UvsX recombinase onto ssDNA and the concomitant displacement of Gp32 ssDNA-binding protein from ssDNA (1,2). Applications include, increasing yield and processivity of RT during RT-PCR and stimulating DNA synthesis in vitro.
Scientific Background: UvsY is a bacteriophage T4 recombination mediator protein (RMP) which promotes homologous recombination. UvsY exists in solution predominantly as a hexamer, and binds to ssDNA in this form. UvsY facilitates assembly of presynaptic filaments by loading UvsX recombinase onto ssDNA and the concomitant displacement of Gp32 ssDNA-binding protein from ssDNA (1,2). Applications include, increasing yield and processivity of RT during RT-PCR and stimulating DNA synthesis in vitro.
Product Categories/Family for T4 recombinant protein
References
1. Liu, J. et al: Mechanism of presynaptic filament stabilization by the bacteriophage T4 UvsY recombination mediator protein. Biochemistry, 2006; 45(17): 5493-5502. 2. Liu, J. et al: Assembly and dynamics of the bacteriophage T4 homologous recombination machinery. Virol J, 2010; 7:357.
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
18kDa
Similar Products
Product Notes
The T4 (Catalog #AAA74074) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "T4 UvsY, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.