Canine TATA Box Binding Protein Associated Factor 2 ELISA Kit | TAF2 elisa kit

Canine TATA Box Binding Protein Associated Factor 2 ELISA Kit

Cat. Num. AAA88989

• Gene Names: TAF2; MRT40; TAF2B; CIF150; TAFII150
• Reactivity: Canine
• Synonyms: TATA Box Binding Protein Associated Factor 2; N/A; Canine TATA Box Binding Protein Associated Factor 2 ELISA Kit; TAF2 elisa kit

• Reactivity: Canine
• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 1.0-25ng/mL
• Sensitivity: 0.1ng/mL

• Intended Uses: This TAF2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine TAF2. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for TAF2 elisa kit

Principle of the Assay: TAF2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TAF2 antibody and an TAF2-HRP conjugate. The assay sample and buffer are incubated together with TAF2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TAF2 concentration since TAF2 from samples and TAF2-HRP conjugate compete for the anti-TAF2 antibody binding site. Since the number of sites is limited, as more sites are occupied by TAF2 from the sample, fewer sites are left to bind TAF2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TAF2 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for TAF2 elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 40674143
• NCBI GeneID: 6873
• Molecular Weight: 136,971 Da
• NCBI Official Full Name: TAF2 protein, partial
• NCBI Official Synonym Full Names: TAF2 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 150kDa
• NCBI Official Symbol: TAF2
• NCBI Official Synonym Symbols: MRT40; TAF2B; CIF150; TAFII150
• NCBI Protein Information: transcription initiation factor TFIID subunit 2; TAF(II)150; TBP-associated factor 150 kDa; 150 kDa cofactor of initiator function; cofactor of initiator function, 150kD subunit; RNA polymerase II TBP-associated factor subunit B; transcription initiation factor TFIID 150 kDa subunit; TATA box binding protein (TBP)-associated factor, RNA polymerase II, B, 150kD
• UniProt Protein Name: Transcription initiation factor TFIID subunit 2
• UniProt Gene Name: TAF2
• UniProt Synonym Gene Names: CIF150; TAF2B; TAF(II)150; TAFII-150; TAFII150
• UniProt Entry Name: TAF2_HUMAN