anti-Human TCR gamma/delta Antibody | anti-TCRgamma/delta antibody

Anti-Hu TCR gamma/delta APC

Cat. Num. AAA129196

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Synonyms: TCR gamma/delta; N/A; Anti-Hu TCR gamma/delta APC; TCRG/D; anti-TCRgamma/delta antibody

• Reactivity: Human
• Isotype: Mouse IgG1
• Clone Number: 11F2
• Specificity: The mouse monoclonal antibody 11F2 recognizes an extracellular epitope in all molecular forms of the TCR gamma/delta.
• Form/Format: APC; Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.; Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 m

• Applicable Applications for anti-TCRgamma/delta antibody: FCM/FACS (Flow Cytometry)
• Immunogen: Purified CD3 antigen complex
• Preparation and Storage: Store at 2-8 degree C. Protect from prolonged exposure to light. Do not freeze.

FCM/FACS (Flow Cytometry)

(Separation of human CD3 positive TCR gamma/delta positive lymphocytes (red-filled) from CD3 negative TCR gamma/delta negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human TCR gamma/delta (11F2) APC antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)

FCM/FACS (Flow Cytometry)

(Flow cytometry multicolor surface staining pattern of human lymphocytes stained using anti-human CD3 (UCHT1) PE antibody (20 ?l reagent / 100 ?l of peripheral whole blood) and anti-human TCR gamma/delta (11F2) APC antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)

FCM/FACS (Flow Cytometry)

(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human TCR gamma/delta (11F2) APC antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)

Related Product Information for anti-TCRgamma/delta antibody

The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.

References

*Ichinohasama R, Miura I, Takahashi T, Yaginuma Y, Myers J, DeCoteau JF, Yee C, Kadin ME, Mori S, Sawai T: Peripheral CD4+ CD8- gammadelta T cell lymphoma: a case report with multiparameter analyses. Hum Pathol. 1996 Dec;27(12):1370-7., URL: https://pubmed.ncbi.nlm.nih.gov/8958314/,*Futagbi G, Welbeck JE, Tetteh JK, Hviid L, Akanmori BD: Selective activation of TCR-gammadelta+ cells in endemic Burkitt's lymphoma. Malar J. 2007 May 23;6:69., URL: https://pubmed.ncbi.nlm.nih.gov/17521425/,*Bertaina A, Zorzoli A, Petretto A, Barbarito G, Inglese E, Merli P, Lavarello C, Brescia LP, De Angelis B, Tripodi G, Moretta L, Locatelli F, Airoldi I: Zoledronic acid boosts gammadelta T-cell activity in children receiving alphabeta + T and CD19 + cell-depleted grafts from an HLA-haplo-identical donor. Oncoimmunology. 2016 Sep 27;6(2):e1216291., URL: https://pubmed.ncbi.nlm.nih.gov/28344861/,*Papadopoulou M, Tieppo P, McGovern N, Gosselin F, Chan JK, Goetgeluk G, Dauby N, Cogan A, Donner C, Ginhoux F, Vandekerckhove B, Vermijlen D: TCR sequencing reveals the distinct development of fetal and adult human Vgamma9Vdelta2 T cells. J Immunol. 2019 Sep 15;203(6):1468-1479., URL: https://pubmed.ncbi.nlm.nih.gov/31413106/,*Borst J, van Dongen JJ, Bolhuis RL, Peters PJ, Hafler DA, de Vries E, van de Griend RJ: Distinct molecular forms of human T cell receptor gamma/delta detected on viable T cells by a monoclonal antibody. J Exp Med. 1988 May 1;167(5):1625-44., URL: https://pubmed.ncbi.nlm.nih.gov/2966845/,