SDS-PAGE
Transforming Growth Factor Alpha (TGFa) Active Protein | TGFa active protein
Active Transforming Growth Factor Alpha (TGFa)
Gene Names
TGFA; TFGA
Reactivity
Human
Applications
Activity Assay, Cell Culture
Purity
> 97%
Synonyms
Transforming Growth Factor Alpha (TGFa); N/A; Active Transforming Growth Factor Alpha (TGFa); ETGF; TGF-A; Protransforming Growth Factor alpha; EGF-Like TGF; TGFa active protein
Host
E Coli
Reactivity
Human
Purity/Purification
> 97%
Form/Format
Freeze-dried powder
Sequence Length
159
Applicable Applications for TGFa active protein
Activity Assay, Cell Culture
Tag
N-terminal His Tag
Buffer Formulation
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Fragment
Glu24~Ala98
Isoelectric Point
6.6
SDS-PAGE
SDS-PAGE
Activity
(Transforming growth factor alpha (TGF-alpha), a ligand for the epidermal growth factor receptor, which activates a signaling pathway for cell proliferation, differentiation and development. To test the effect of TGF-alpha on cell proliferation of 3T3 fibroblasts, 3T3 cells were seeded into triplicate wells of 96-well plates at a density of 2, 000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of TGF-alpha. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37 degree C.Cell proliferation of 3T3 cells after incubation with TGF-alpha for 72h observed by inverted microscope was shown in Figure 1.)
Activity
(Transforming growth factor alpha (TGF-alpha), a ligand for the epidermal growth factor receptor, which activates a signaling pathway for cell proliferation, differentiation and development. To test the effect of TGF-alpha on cell proliferation of 3T3 fibroblasts, 3T3 cells were seeded into triplicate wells of 96-well plates at a density of 2, 000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of TGF-alpha. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37 degree C.Cell proliferation of 3T3 cells after incubation with TGF-alpha for 72h observed by inverted microscope was shown in Figure 1.)
Product Categories/Family for TGFa active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted Molecular Weight: 11.7kDa
Accurate Molecular Weight: 15kDa
Accurate Molecular Weight: 15kDa
NCBI Official Full Name
protransforming growth factor alpha isoform 2 preproprotein
NCBI Official Synonym Full Names
transforming growth factor alpha
NCBI Official Symbol
TGFA
NCBI Official Synonym Symbols
TFGA
NCBI Protein Information
protransforming growth factor alpha
UniProt Protein Name
Protransforming growth factor alpha
UniProt Gene Name
TGFA
UniProt Synonym Gene Names
TGF-alpha; ETGF
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Product Notes
The TGFa tgfa (Catalog #AAA148232) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The Active Transforming Growth Factor Alpha (TGFa) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Transforming Growth Factor Alpha (TGFa) can be used in a range of immunoassay formats including, but not limited to, Activity Assay, Cell Culture. Researchers should empirically determine the suitability of the TGFa tgfa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Transforming Growth Factor Alpha (TGFa), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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