Standard Curve (Sample)
TGF Alpha ELISA Kit | TGFA elisa kit
Horse equine TGF Alpha PicoKine ELISA Kit
Gene Names
TGFA; TFGA
Reactivity
EquineThere is cross-reactivity with TGF beta 2, TGF beta 3, TGF beta 5<1%.
Applications
ELISA
Synonyms
TGF Alpha; N/A; Horse equine TGF Alpha PicoKine ELISA Kit; Protransforming growth factor alpha; Transforming growth factor alpha; TGF-alpha; EGF-like TGF; ETGF; TGF type 1; TGFA; TGFA elisa kit
Reactivity
Equine
There is cross-reactivity with TGF beta 2, TGF beta 3, TGF beta 5<1%.
There is cross-reactivity with TGF beta 2, TGF beta 3, TGF beta 5<1%.
Specificity
Natural and recombinant 216 Horse equine TGF Alpha
Sequence Length
162
Assay Type
Quantitative Sandwich
Detection Range
15.6pg/ml-1000pg/ml
Sensitivity
<1pg/ml
Applicable Applications for TGFA elisa kit
ELISA
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration weretested on one plate to assess intra-assay precision
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration weretested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for TGFA elisa kit
Principle of the Assay: 216 Horse equine TGF Alpha ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Horse equine TGF Alpha has been precoated onto 96-well plates. Standards(Expression system for standard: E. coli,V40-A89) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Horse equine TGF Alpha is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the 216 Horse equine TGF Alpha amount of sample captured in plate.
Background: Transforming growth factor alpha(TGF-alpha) is upregulated in some horse equine cancers. It is produced in macrophages, brain cells, and keratinocytes, and induces epithelial development. It is closely related to EGF, and can also bind to the EGF receptor with similar effects. TGFalpha stimulates neural cell proliferation in the adult injured brain. Transforming growth factor alpha gene(TGFA) maps to horse equine chromosome 2 close to the breakpoint of the t(2;8) variant translocation in Burkitt lymphoma. Synthetic TGF-alpha was as active as murine epidermal growth factor in binding to the epidermal growth factor receptor and in stimulation of anchorage-dependent and of anchorage-independent growth of normal indicator cells in culture. Synthetic TGF-alpha stimulated plasminogen activator production in A 431 and HeLa cells; the stimulation was similar to that induced by epidermal growth factor. Furthermore, synthetic horse equine TGF-alpha showed similar immunoreactivity when compared with horse equine TGF-alpha. Thus, the 50-amino acid TGF- alpha is likely to be the bioactive principle produced and secreted by tumor cell lines.
Background: Transforming growth factor alpha(TGF-alpha) is upregulated in some horse equine cancers. It is produced in macrophages, brain cells, and keratinocytes, and induces epithelial development. It is closely related to EGF, and can also bind to the EGF receptor with similar effects. TGFalpha stimulates neural cell proliferation in the adult injured brain. Transforming growth factor alpha gene(TGFA) maps to horse equine chromosome 2 close to the breakpoint of the t(2;8) variant translocation in Burkitt lymphoma. Synthetic TGF-alpha was as active as murine epidermal growth factor in binding to the epidermal growth factor receptor and in stimulation of anchorage-dependent and of anchorage-independent growth of normal indicator cells in culture. Synthetic TGF-alpha stimulated plasminogen activator production in A 431 and HeLa cells; the stimulation was similar to that induced by epidermal growth factor. Furthermore, synthetic horse equine TGF-alpha showed similar immunoreactivity when compared with horse equine TGF-alpha. Thus, the 50-amino acid TGF- alpha is likely to be the bioactive principle produced and secreted by tumor cell lines.
Product Categories/Family for TGFA elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17006 MW
NCBI Official Full Name
protransforming growth factor alpha isoform 2 preproprotein
NCBI Official Synonym Full Names
transforming growth factor alpha
NCBI Official Symbol
TGFA
NCBI Official Synonym Symbols
TFGA
NCBI Protein Information
protransforming growth factor alpha
UniProt Protein Name
Protransforming growth factor alpha
UniProt Gene Name
TGFA
UniProt Synonym Gene Names
TGF-alpha; ETGF
Similar Products
Product Notes
The Equine There is cross-reactivity with TGF beta 2, TGF beta 3, TGF beta 5<1%. TGFA tgfa (Catalog #AAA124787) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA124787 ELISA Kit recognizes Equine There is cross-reactivity with TGF beta 2, TGF beta 3, TGF beta 5<1%. TGFA. AAA Biotech's TGF Alpha can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the TGFA tgfa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TGF Alpha, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.