Standard Curve (Sample)
Human TGF beta 1 ELISA Kit | TGFB1 elisa kit
Human TGF beta 1 PicoKine ELISA Kit
Gene Names
TGFB1; CED; LAP; DPD1; TGFB; TGFbeta
Reactivity
Human
Synonyms
TGF beta 1; N/A; Human TGF beta 1 PicoKine ELISA Kit; Transforming growth factor beta-1; transforming growth factor, beta 1; Camurati-Engelmann disease; CED; DPD1; LAP; Latency-associated peptide; TGF beta 1 protein; TGF-beta-1; TGFB; tgfb1; TGFB1_HUMAN; TGFbeta; Transforming growth factor beta 1; TGFB1 elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human TGFB1
Sequence Length
390
Samples
Cell culture supernatants, serum, plasma (EDTA) and urine
Detection Range
15.6 pg/ml - 1,000 pg/ml
Sensitivity
<1pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for TGFB1 elisa kit
Background: Transforming growth factor-beta1 (TGF-beta1) is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. Many cells synthesize TGF-beta and essentially all of them have specific receptors for this peptide. TGF-beta regulates the actions of many other peptide growth factors and determines a positive or negative direction of their effects.TGFbeta1 is known for its potent and diverse biological effects, including immune regulation, and cell growth and differentiation.TGFbeta1 is also an important mediator of bone remodeling.TGFbeta1, a potent keratinocyte growth inhibitor, has been shown to be overexpressed in keratinocytes in certain inflammatory skin diseases and has been thought to counteract the effects of other growth factors at the site of inflammation.TGF-beta1, a multifunctional cytokine with fibrogenic properties, has been implicated in the pathogenesis of the vascular and target organ complications of hypertension. TGF-beta1 may also regulate blood pressure via stimulation of endothelin-1 and/or renin secretion. TGFbeta1 is secreted as a latent form, which consists of its mature form and a latency-associated peptide (beta1-LAP) in either the presence or the absence of additional latent TGF-beta1-binding protein. The standard product used in this kit is recombinant TGFbeta1 with the molecular mass of 25KDa.
Principle of the Assay: The Picokine™ Human TGFB1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human TGFB1 with a 96-well strip plate that is pre-coated with antibody specific for TGFB1. The detection antibody is a biotinylated antibody specific for TGFB1. The capture antibody is polyclonal antibody from chicken and the detection antibody is polyclonal antibody from chicken. The kit contains recombinant Human TGFB1 with immunogen: Expression system for standard: CHO; Immunogen sequence: A279-S390. The kit is analytically validated with ready-to-use reagents. To measure Human TGFB1, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human TGFB1 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human TGFB1 in the sample.
Principle of the Assay: The Picokine™ Human TGFB1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human TGFB1 with a 96-well strip plate that is pre-coated with antibody specific for TGFB1. The detection antibody is a biotinylated antibody specific for TGFB1. The capture antibody is polyclonal antibody from chicken and the detection antibody is polyclonal antibody from chicken. The kit contains recombinant Human TGFB1 with immunogen: Expression system for standard: CHO; Immunogen sequence: A279-S390. The kit is analytically validated with ready-to-use reagents. To measure Human TGFB1, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human TGFB1 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human TGFB1 in the sample.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
44,341 Da
NCBI Official Full Name
Transforming growth factor beta-1
NCBI Official Synonym Full Names
transforming growth factor, beta 1
NCBI Official Symbol
TGFB1
NCBI Official Synonym Symbols
CED; LAP; DPD1; TGFB; TGFbeta
NCBI Protein Information
transforming growth factor beta-1; TGF-beta-1; latency-associated peptide; prepro-transforming growth factor beta-1
UniProt Protein Name
Transforming growth factor beta-1
UniProt Gene Name
TGFB1
UniProt Synonym Gene Names
TGFB; TGF-beta-1; LAP
UniProt Entry Name
TGFB1_HUMAN
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Product Notes
The Human TGFB1 tgfb1 (Catalog #AAA11551) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA11551 ELISA Kit recognizes Human TGFB1. It is sometimes possible for the material contained within the vial of "TGF beta 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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