Standard Curve (Sample)
TGF Beta 1 ELISA Kit | TGFB1 elisa kit
Rabbit TGF Beta 1 PicoKine ELISA Kit
Gene Names
TGFB1; CED; LAP; DPD1; TGFB; TGFbeta
Reactivity
RabbitThere is cross-reactivity with TGF beta 2, TGF beta 3, TGF beta 5<1%.
Applications
ELISA
Synonyms
TGF Beta 1; N/A; Rabbit TGF Beta 1 PicoKine ELISA Kit; Transforming growth factor beta-1; TGF-beta-1; Latency-associated peptide; LAP; TGFB1; TGFB; TGFB1 elisa kit
Reactivity
Rabbit
There is cross-reactivity with TGF beta 2, TGF beta 3, TGF beta 5<1%.
There is cross-reactivity with TGF beta 2, TGF beta 3, TGF beta 5<1%.
Specificity
Natural and recombinant Mouse TNFSF13B
Sequence Length
390
Samples
Cell Culture Supernates, Cell Lysates, Serum And Plasma (Heparin, Edta)
Detection Range
<10pg/ml
Sensitivity
46.9pg/ml-3000pg/ml
Applicable Applications for TGFB1 elisa kit
ELISA
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-assay Precision
Inter-Assay Precision (Precision across assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for TGFB1 elisa kit
Principle of the Assay: The Picokine Mouse TNFSF13B Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse TNFSF13B with a 96-well strip plate that is pre-coated with antibody specific for TNFSF13B. The detection antibody is a biotinylated antibody specific for TNFSF13B. The capture antibody is monoclonal antibody from rat, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Mouse TNFSF13B with immunogen: Expression system for standard: NSO; Immunogen sequence: A127-L309. The kit is analytically validated with ready to use reagents. To measure Mouse TNFSF13B, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Mouse TNFSF13B in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse TNFSF13B in the sample!!Background/Introduction: B-cell activating factor(BAFF) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFLSF13B gene.1,2 BAFF is a cytokine that belongs to the tumor necrosis factor(TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells.3All these ligands act as heterotrimers(i.e. three of the same molecule) interacting with heterotrimeric receptors4, although BAFF has been known to be active as either a hetero-or homotrimer.5 The standard used in this kit is recombinant mouse soluble BAFF(A127-L309) with molecular weight 23.2KDa.
Product Categories/Family for TGFB1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
44341 MW
NCBI Official Full Name
transforming growth factor beta-1 preproprotein
NCBI Official Synonym Full Names
transforming growth factor beta 1
NCBI Official Symbol
TGFB1
NCBI Official Synonym Symbols
CED; LAP; DPD1; TGFB; TGFbeta
NCBI Protein Information
transforming growth factor beta-1
UniProt Protein Name
Transforming growth factor beta-1
UniProt Gene Name
TGFB1
UniProt Synonym Gene Names
TGFB; TGF-beta-1; LAP
Similar Products
Product Notes
The Rabbit There is cross-reactivity with TGF beta 2, TGF beta 3, TGF beta 5<1%. TGFB1 tgfb1 (Catalog #AAA124795) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA124795 ELISA Kit recognizes Rabbit There is cross-reactivity with TGF beta 2, TGF beta 3, TGF beta 5<1%. TGFB1. AAA Biotech's TGF Beta 1 can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the TGFB1 tgfb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TGF Beta 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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