TLR4 / CD284 recombinant protein

Recombinant Human TLR4 / CD284 Protein (His tag)

Cat. Num. AAA173573

• Gene Names: TLR4; TOLL; CD284; TLR-4; ARMD10
• Purity: >87 % as determined by reducing SDS-PAGE.
• Synonyms: TLR4 / CD284; N/A; Recombinant Human TLR4 / CD284 Protein (His tag); ARMD10; CD284; TLR-4; TLR4; TOLL; TLR4 / CD284 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >87 % as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from sterile 20mM PB, 500mM NaCl, 10% Glycerol, pH 6.0, 5%Trehalose, 5%Mannitol, 0.01% Tween-80
• Sequence Positions: Met 1-Lys631
• Sequence Length: 799

• Species: Human
• Reconstitution: We recommend that the vial be centrifuged to opening to bring the contents to the bottom. Do not mix by vortex. It is recommended that sterile water be added to the vial to prepare a stocksolution of 0.25 mg/mL. Concentration is measured by UV-Vis.
• Endotoxin: < 1.0 EU per ug as determined by the LAL method.
• Tag: C-His
• Preparation and Storage: Storage: Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4°C to 8°C for 2-7 days. Aliquots of reconstituted samples are stable at <-20 degree C for 3 months.

SDS-PAGE

Related Product Information for TLR4 / CD284 recombinant protein

TLR4, also known as TLR-4, is a member of the Toll-like receptor (TLR) family, which plays a fundamental role inpathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and sharestructural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressedon infectious agents, and mediate the production of cytokines necessary for the development of effective immunity.TLR4 is most abundantly expressed in placenta, and in myelomonocytic subpopulation of the leukocytes. TLR 4 has alsobeen designated as CD284 (cluster of differentiation 284). It has been implicated in signal transduction events induced bylipopolysaccharide (LPS) found in most gram-negative bacteria. TLR4 Cooperates with LY96 and CD14 to mediate theinnate immune response to bacterial lipopolysaccharide (LPS). It acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. It is also involved in LPS-independentinflammatory responses triggered by Ni(2+).

NCBI and Uniprot Product Information

• NCBI GI #: 4507533
• NCBI GeneID: 7099
• NCBI Accession #: NP_003257.1
• NCBI GenBank Nucleotide #: NM_003266.3
• UniProt Accession #: O00206-1; Q5VZI8; Q5VZI9; Q9UK78; Q9UM57; A8K1Y8; A9XLP9; A9XLQ0; A9XLQ1; B4E194; D1CS52; D1CS53; O00206
• Molecular Weight: Mol_Mass: 70.5 kDa; AP_Mol_Mass: 68 kDa
• NCBI Official Full Name: toll-like receptor 4 isoform C
• NCBI Official Synonym Full Names: toll like receptor 4
• NCBI Official Symbol: TLR4
• NCBI Official Synonym Symbols: TOLL; CD284; TLR-4; ARMD10
• NCBI Protein Information: toll-like receptor 4
• UniProt Protein Name: Toll-like receptor 4
• UniProt Gene Name: TLR4