Standard Curve (Sample)
Tumor Necrosis Factor alpha ELISA Kit | TNF-a elisa kit
Tumor Necrosis Factor alpha (TNF-a)
Gene Names
TNF; DIF; TNFA; TNFSF2; TNF-alpha
Synonyms
Tumor Necrosis Factor alpha; N/A; Tumor Necrosis Factor alpha (TNF-a); Tumor Necrosis Factor alpha (TNF-alpha); TNF-a elisa kit
Specificity
This sandwich ELISA recognises both natural and recombinant human TNF-alpha. This kit exhibits no significant cross-reactivity with human TGF, MCP-1, MCP-3, M-CSF, GM-CSF, EGF, IL-1beta, IL-8, and IL-16.
Sequence Length
233
Assay Type
Quantitative Sandwich
Samples
Cell culture supernatant, serum, plasma, and other biological fluids
Sensitivity
2.4pg/mL
Intra-assay Precision
To determine within-run precision, three different samples of known concentration were assayed by replicates of twenty in 1 assay.
Inter-assay Precision
To determine between-run precision, three different samples of known concentration were assayed by replicates on 20 different assays.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for TNF-a elisa kit
Background: Tumour Necrosis Factor alpha (TNF-alpha), also known as cachectin was initially named for its remarkable ability to cause hemorrhagic necrosis of tumours in mice.1 The TNF-alpha gene located on chromosome 6 encodes a 233 amino acid (a.a.) prohormone bound to the plasma membrane, with the mature form (157a.a., 17kDa) exposed on the extracellular surface. Soluble, mature TNF-alpha is released upon cleavage of the C-terminal.2,3 The primary source in vivo of TNF-alpha is thought to be the monocyte/macrophage but various cell types are known to express this cytokine such as lymphocytes, basophils, eosinophils, mast cells, NK cells, T cells, B cells, kerantinocytes, Kupffer cells, astrocytes, and some types of tumours. TNF-alpha is produced upon stimulation with cytokines such as IL-1, 1L-2, GM-CSF, TNF-alpha itself and with bacterial lipopolysaccharide (LPS) which is a potent inducer.4 TNF-alpha once produced and secreted will bind to TNF-alpha receptors (TNF-alpha R1, 55 kDa and TNF-alpha R2, 75 kDa), located on the plasma membrane of most cells throughout the body except the red blood cell.5 The two TNF-alpha receptors deliberate the biological effects of TNF-alpha. It has been reported that TNF-alpha R1 is responsible for mediating LPS toxicity6 and cell cytotoxicity7 and TNF-alpha R2 is involved in cellular proliferation.8 Various pathological conditions are associated with the production of high levels of TNF-alpha. These include septic shock syndrome, cachexia (e.g. HIV, tuberculosis, cancer), autoimmune diseases, hepatitis, leukemia, myocardial ischaemia, organ transplantation rejection, multiple sclerosis, rheumatoid arthritis, and meningococcal septicemia.4,9,10,11 Many people die annually from a complication of infectious disease called septic shock syndrome which is triggered by TNF-alpha. In many cases elevated TNF-alpha serum levels predict a higher mortality.12 TNF-alpha is a pleiotropic cytokine that can induce disease through TNF-alpha toxicity (tissue injury, catabolic illness, and mediating shock) and improve host defense mechanisms (stimulating inflammation and increasing immune cell function).4 In the future, therapies may be developed by blocking TNF-alpha harmful effects and enhancing TNF-alpha beneficial effects.
Principle of the Assay: This TNF-alpha enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to TNF-alpha. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated monoclonal antibody preparation specific for TNF-alpha and incubated. TNF-alpha, if present, will bind and become immobilized by the antibody pre-coated on the wells and then be "sandwiched" by biotin conjugate. The microtiter plate wells are thoroughly washed to remove unbound TNF-alpha and other components of the sample. In order to quantify the amount of TNF-alpha present in the sample, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Avidin is a tetramer containing four identical subunits that each has a high affinity-binding site for biotin. The wells are thoroughly washed to remove all unbound HRP-conjugated Avidin and a TMB (3,3'5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TNF-alpha, biotin-conjugated antibody, and enzyme-conjugated Avidin will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. In order to measure the concentration of TNF-alpha in the samples, this kit includes two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant testing.) According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D) versus TNF-alpha concentration (pg/mL). The concentration of TNF-alpha in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Intended Uses: This Human TNF-alpha (alpha) ELISA Kit is to be used for the in vitro quantitative determination of human tumour necrosis factor alpha (TNF-alpha) concentrations in cell culture supernatant, serum, plasma, and other biological fluids. This kit is intended FOR LABORATORY RESEARCH USE ONLY and not for use in test or therapeutic procedures.
Principle of the Assay: This TNF-alpha enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific to TNF-alpha. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated monoclonal antibody preparation specific for TNF-alpha and incubated. TNF-alpha, if present, will bind and become immobilized by the antibody pre-coated on the wells and then be "sandwiched" by biotin conjugate. The microtiter plate wells are thoroughly washed to remove unbound TNF-alpha and other components of the sample. In order to quantify the amount of TNF-alpha present in the sample, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Avidin is a tetramer containing four identical subunits that each has a high affinity-binding site for biotin. The wells are thoroughly washed to remove all unbound HRP-conjugated Avidin and a TMB (3,3'5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TNF-alpha, biotin-conjugated antibody, and enzyme-conjugated Avidin will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. In order to measure the concentration of TNF-alpha in the samples, this kit includes two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant testing.) According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D) versus TNF-alpha concentration (pg/mL). The concentration of TNF-alpha in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Intended Uses: This Human TNF-alpha (alpha) ELISA Kit is to be used for the in vitro quantitative determination of human tumour necrosis factor alpha (TNF-alpha) concentrations in cell culture supernatant, serum, plasma, and other biological fluids. This kit is intended FOR LABORATORY RESEARCH USE ONLY and not for use in test or therapeutic procedures.
Product Categories/Family for TNF-a elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
25,644 Da
NCBI Official Full Name
TNF-alpha
NCBI Official Synonym Full Names
tumor necrosis factor
NCBI Official Symbol
TNF
NCBI Official Synonym Symbols
DIF; TNFA; TNFSF2; TNF-alpha
NCBI Protein Information
tumor necrosis factor; APC1 protein; TNF, macrophage-derived; TNF, monocyte-derived; TNF-a; cachectin; tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha
UniProt Protein Name
Tumor necrosis factor
UniProt Gene Name
TNF
UniProt Entry Name
TNFA_HUMAN
Similar Products
Product Notes
The TNF-a tnf (Catalog #AAA77862) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Tumor Necrosis Factor alpha, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.