Bioactivity
(Bovine TNF-alpha stimulates cell cytotoxicity in the L929 mouse fibrosarcoma cells. The ED50 range )
TNF-alpha recombinant protein
Recombinant bovine TNF-alpha protein
Applications
SDS-Page
Purity
> 90% by SDS-PAGE
Synonyms
TNF-alpha; N/A; Recombinant bovine TNF-alpha protein; Tumor necrosis factor; TNF-a; TNF; TNFa; TNF-alpha recombinant protein
Host
E Coli
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Concentration
1mg/ml (determined by Bradford assay) (varies by lot)
Sequence Positions
78-234aa
Sequence
MLRSSSQASS NKPVAHVVAD INSPGQLRWW DSYANALMAN GVKLEDNQLV VPADGLYLIY SQVLFRGQGC PSTPLFLTHT ISRIAVSYQT KVNILSAIKS PCHRETPEWA EAKPWYEPIY QGGVFQLEKG DRLSAEINLP DYLDYAESGQ VYFGIIAL
Applicable Applications for TNF-alpha recombinant protein
Bioactivity, SDS-PAGE
Species
Bovine
Tag
Non-Tagged
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Bioactivity
Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 range <= 15ng/ml.
Preparation and Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Bioactivity
(Bovine TNF-alpha stimulates cell cytotoxicity in the L929 mouse fibrosarcoma cells. The ED50 range )
Bioactivity
(Bovine TNF-alpha stimulates cell cytotoxicity in the L929 mouse fibrosarcoma cells. The ED50 range )
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
Related Product Information for TNF-alpha recombinant protein
TNF-alpha, also known as tumor necrosis factor, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. This protein is produced by a wide variety of immune and epithelial cell types. It is assembled intracellularly to form a noncovalently linked homotrimer which is expressed on the cell surface. Cell surface TNF can induce the lysis of neighboring tumor cells and virus infected cells, and it can generate its own downstream cell signaling following ligation by soluble TNFR I. It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages. Recombinant Bovine TNF-alpha was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for TNF-alpha recombinant protein
References
Bojarojc-Nosowicz B., et al (2015). Pol J Vet Sci. 18(3): 533-9.;;Lendez PA., et al (2015). Arch Virol. 160(8): 2001-7.
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
17.5kDa (158aa) confirmed by MALDI-TOF
UniProt Protein Name
Tumor necrosis factor
UniProt Gene Name
TNF
UniProt Synonym Gene Names
TNFA; TNFSF2; TNF-a; NTF; ICD1; ICD2
UniProt Entry Name
TNFA_BOVIN
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Product Notes
The TNF-alpha tnf (Catalog #AAA48420) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 78-234aa. AAA Biotech's TNF-alpha can be used in a range of immunoassay formats including, but not limited to, Bioactivity, SDS-PAGE. Researchers should empirically determine the suitability of the TNF-alpha tnf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MLRSSSQASS NKPVAHVVAD INSPGQLRWW DSYANALMAN GVKLEDNQLV VPADGLYLIY SQVLFRGQGC PSTPLFLTHT ISRIAVSYQT KVNILSAIKS PCHRETPEWA EAKPWYEPIY QGGVFQLEKG DRLSAEINLP DYLDYAESGQ VYFGIIAL. It is sometimes possible for the material contained within the vial of "TNF-alpha, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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