Tumor Necrosis Factor-alpha Recombinant Protein | TNF recombinant protein

Mouse Tumor Necrosis Factor-alpha Recombinant

Cat. Num. AAA76088

• Purity: >97%, as determined by SDS-PAGE and HPLC
• Synonyms: Tumor Necrosis Factor-alpha; N/A; Mouse Tumor Necrosis Factor-alpha Recombinant; tnf-alpha; tumor necrosis factor; cachetin; tnfa; TNF recombinant protein

• Host: E Coli (Host protein expression may be lot-specific and can be subject to change during production, including bulk orders)
• Purity/Purification: >97%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant mouse TNF alpha was lyophilized from 0.2 um filtered PBS solution, pH 7.0.
• Sequence: MSTESMIRDV ELAEEALPQK MGGFQNSRRC LCLSLFSFLL VAGATTLFCL LNFGVIGPQR DEKFPNGLPL ISSMAQTLTL RSSSQNSSDK PVAHVVANHQ VEEQLEWLSQ RANALLANGM DLKDNQLVVP ADGLYLVYSQ VLFKGQGCPD YVLLTHTVSR FAISYQEKVN LLSAVKSPCP KDTPEGAELK PWYEPIYLGG VFQLEKGDQL SAEVNLPKYL DFAESGQVYF GVIAL
• Sequence Length: 235

• Domain: TNF
• Host Note: Optimized DNA sequence encoding Mouse Tumor Necrosis Factor-alpha extracellular domain was expressed in Escherichia Coli.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Molecular Weight Note: Native Mouse Tumor Necrosis Factor-alpha is generated by the proteolytic removal of the signal peptide and propeptide, this molecule has a calculated mass of approximately 17kDa.; ; Recombinant Mouse TNF is a disulfide-linked monomer protein, consisting of 157 amino acids and migrates as an approximately 17 kDa protein under reducing conditions.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.; Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Related Product Information for TNF recombinant protein

TNF is secreted by macrophages, monocytes, neutrophils, T-cells, NK- cells following their stimulation by bacterial lipopolysaccharides. Cells expressing CD4 secrete TNF- alpha while CD8 (+) cells secrete little or no TNF- alpha. Stimulated peripheral neutrophilic granulocytes but also unstimulated cells and also a number of transformed cell lines, astrocytes, microglial cells, smooth muscle cells, and fibroblasts also secrete TNF. Human milk also contains this factor.

Product Categories/Family for TNF recombinant protein

Cytokines

References

• tak1-mediated serine/threonine phosphorylation of epidermal growth factor receptor via p38/extracellular signal-regulated kinase: nf-b-independent survival pathways in tumor necrosis factor alpha signaling .Mol. Cell. Biol., Oct 2009; 29: 5529 - 5539.
• tumor necrosis factor--mediated regulation of the inositol 1,4,5-trisphosphate receptor promoter .J. Biol. Chem., Oct 2009; 284: 27557 - 27566.
• tnf- inhibition attenuates adverse myocardial remodeling in a rat model of volume overload .Am J Physiol Heart Circ Physiol, Oct 2009; 297: H1462 - H1468.
• tnf inhibits apoptotic cell clearance in the lung, exacerbating acute inflammation .Am J Physiol Lung Cell Mol Physiol, Oct 2009; 297: L586 - L595.
• association of tnf- gene polymorphisms with systemic lupus erythematosus in taiwanese patients .Lupus, Oct 2009; 18: 974 - 979.