Application Data
(Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 5-50 pg/mL.)
TNF-alpha Recombinant Protein | TNF recombinant protein
TNF-alpha Protein, Human, Recombinant
Gene Names
TNF; DIF; TNFA; TNFSF2; TNF-alpha
Reactivity
Human
Purity
>95 % as determined by SDS-PAGE and SEC-HPLC
Synonyms
TNF-alpha; N/A; TNF-alpha Protein, Human, Recombinant; Human TNF-alpha/TNFA Protein; tumor necrosis factor; DIF Protein, Human; TNF-alpha Protein, Human; TNFA Protein, Human; TNFSF2 Protein, Human; TNF recombinant protein
Host
E Coli
Reactivity
Human
Purity/Purification
>95 % as determined by SDS-PAGE and SEC-HPLC
Form/Format
Lyophilized from sterile 20mM Tris, 500mM NaCI, 10% Glycerol, pH 7.4, 5% trehalose, 5% mannitol, 0.01% tween-80.
Sequence Positions
158aa
Sequence
Val77-Leu233
Source
Human
Predicted N Terminal
Met
Reconstitution
It is recommended that sterile water (400ul) be added to the vial to prepare a stock solution of 0.25mg/mL.
Concentration is mesured by UV.
Concentration is mesured by UV.
Protein Construction
A DNA sequence encoding the human TNF-alpha soluble form (NP_000585.2) (Val 77-Leu 233) was expressed, with an initial Met at the N-terminus
Bio Activity
1. Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 5-50 pg/mL.
Endotoxin
< 5 EU per mg of the protein as determined by LAL method.
Preparation and Storage
Samples are stable for twelve months from date of receipt at -20°C to -80°C.
Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage.
Avoid repeated freeze-thaw cycles.
Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage.
Avoid repeated freeze-thaw cycles.
Application Data
(Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 5-50 pg/mL.)
Application Data
(Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 5-50 pg/mL.)
FCM/FACS (Flow Cytometry)
(Labeled biotin to TNF-alpha Protein, Human, Recombinant by a certain molar ratio; Using the Octet RED System, the affinity constant (Kd) of TNF-alpha Protein, Human, Recombinant, Biotinylated bound to Humira was 0.1 nM.)
FCM/FACS (Flow Cytometry)
(Labeled biotin to TNF-alpha Protein, Human, Recombinant by a certain molar ratio; Using the Octet RED System, the affinity constant (Kd) of TNF-alpha Protein, Human, Recombinant, Biotinylated bound to Humira was 0.1 nM.)
Application Data
Application Data
Related Product Information for TNF recombinant protein
Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment.
TNF-alpha Protein & Antibody
Product Categories/Family for TNF recombinant protein
References
Hector J, et al. (2007) TNF-alpha alters visfatin and adiponectin levels in human fat. Horm Metab Res. 39(4): 250-5.
Berthold-Losleben M, et al. (2008) The TNF-alpha System: Functional Aspects in Depression, Narcolepsy and Psychopharmacology. Curr Neuropharmacol. 6(3): 193-202.
Berthold-Losleben M, et al. (2008) The TNF-alpha System: Functional Aspects in Depression, Narcolepsy and Psychopharmacology. Curr Neuropharmacol. 6(3): 193-202.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
The reconbinant human TNF-α consists of 150 amino acids and has a predicted molecular mass of 17.4 kDa whichis also estimated by SDS-PAGE.
NCBI Official Full Name
tumor necrosis factor
NCBI Official Synonym Full Names
tumor necrosis factor
NCBI Official Symbol
TNF
NCBI Official Synonym Symbols
DIF; TNFA; TNFSF2; TNF-alpha
NCBI Protein Information
tumor necrosis factor; TNF-a; cachectin; APC1 protein; TNF, monocyte-derived; TNF, macrophage-derived; tumor necrosis factor-alpha; tumor necrosis factor ligand superfamily member 2
UniProt Protein Name
Tumor necrosis factor
UniProt Gene Name
TNF
UniProt Synonym Gene Names
TNFA; TNFSF2; TNF-a; NTF; ICD1; ICD2
UniProt Entry Name
TNFA_HUMAN
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Product Notes
The TNF tnf (Catalog #AAA257428) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 158aa. The TNF-alpha Protein, Human, Recombinant reacts with Human and may cross-react with other species as described in the data sheet. The amino acid sequence is listed below: Val77-Leu2 33. It is sometimes possible for the material contained within the vial of "TNF-alpha, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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