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product-image-AAA76268_AD13.jpg Application Data (Biotinylated Human TNF alpha Protein SEC-HPLCThe purity of Biotinylated Human TNF alpha is greater than 95% as determined by SEC-HPLC.)

TNF alpha Recombinant Protein | TNFalpha recombinant protein

Biotinylated Human TNF alpha Protein Recombinant

Gene Names
TNF; DIF; TNFA; TNFSF2; TNF-alpha
Purity
>95%, as determined by SDS-PAGE and HPLC
Synonyms
TNF alpha; N/A; Biotinylated Human TNF alpha Protein Recombinant; TNFalpha recombinant protein
Ordering
Host
HEK293 cells (Note: Host protein expression may be lot-specific and can be subject to change during production, including bulk orders.)
Purity/Purification
>95%, as determined by SDS-PAGE and HPLC
Form/Format
Recombinant Human TNF alpha was lyophilized from 0.2 um filtered PBS solution pH7.4
Source
DNA sequence encoding Human TNF alpha including a C-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
Molecular Function
Cytokine
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM
Preparation and Storage
The lyophilized protein is stable for at least 2 years from date of receipt at-20 degree C.

Application Data

(Biotinylated Human TNF alpha Protein SEC-HPLCThe purity of Biotinylated Human TNF alpha is greater than 95% as determined by SEC-HPLC.)

product-image-AAA76268_AD13.jpg Application Data (Biotinylated Human TNF alpha Protein SEC-HPLCThe purity of Biotinylated Human TNF alpha is greater than 95% as determined by SEC-HPLC.)

Tris-Bis-PAGE

(Biotinylated Human TNF alpha Protein Tris-Bis PAGEBiotinylated Human TNF alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.)

product-image-AAA76268_PAGE15.jpg Tris-Bis-PAGE (Biotinylated Human TNF alpha Protein Tris-Bis PAGEBiotinylated Human TNF alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.)
Product Categories/Family for TNFalpha recombinant protein

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,644 Da
NCBI Official Full Name
tumor necrosis factor
NCBI Official Synonym Full Names
tumor necrosis factor
NCBI Official Symbol
TNF
NCBI Official Synonym Symbols
DIF; TNFA; TNFSF2; TNF-alpha
NCBI Protein Information
tumor necrosis factor; TNF-a; cachectin; APC1 protein; TNF, monocyte-derived; TNF, macrophage-derived; tumor necrosis factor-alpha; tumor necrosis factor ligand superfamily member 2
UniProt Protein Name
Tumor necrosis factor
UniProt Gene Name
TNF
UniProt Synonym Gene Names
TNFA; TNFSF2; TNF-a; NTF; ICD1; ICD2
UniProt Entry Name
TNFA_HUMAN

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Product Notes

The TNFalpha tnf (Catalog #AAA76268) is a Recombinant Protein produced from HEK293 cells (Note: Host protein expression may be lot-specific and can be subject to change during production, including bulk orders.) and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "TNF alpha, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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