Mouse sRANK Ligand CLIA Kit | TNFRSF11a clia kit

sRANK Ligand (Murine) LumiAb ELISA Kit

Cat. Num. AAA318581

• Gene Names: Tnfrsf11a; OFE; ODFR; Rank; Ly109; mRANK; TRANCE-R
• Reactivity: Mouse
• Synonyms: sRANK Ligand; N/A; sRANK Ligand (Murine) LumiAb ELISA Kit; Murine sRANK Ligand; Tumor necrosis factor receptor superfamily member 11A; Osteoclast differentiation factor receptor; ODFR; Receptor activator of NF-KB; CD265; Rank; TNFRSF11a clia kit

• Reactivity: Mouse
• Specificity: The LumiAb Murine SCF ELISA is capable of recognizing both recombinant and naturally produced Murine SCF proteins. The antigens listed below were tested at 50 ng/ml and exhibited 100% cross reactivity.
• Sequence Length: 625
• Assay Type: Quantitative Sandwich
• Sensitivity: 8 pg/ml.

• Preparation and Storage: Store at 4 degree C for 6 months.

Standard Curve (Sample)

Related Product Information for TNFRSF11a clia kit

Principle of the Assay: The LumiAb Murine SCF Chemiluminescent ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Murine SCF concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Murine SCF while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a sensitive luminescent reaction to ensue upon substrate addition. When the Peroxide Enhancer solution is added, the reaction catalyzed by peroxidase yields light that is representative of the antigen concentration. After a brief incubation, the microplate can be read with a luminometer, allowing for generation of a standard curve and subsequent determination of protein concentration!!Background/Introduction: Murine SCF or Stem Cell Factor, also known as Kit Ligand, is a 273 amino acid cytokine protein expressed from the Kitl gene located on chromosome 10. After initial synthesis and translocation, the 25 residue signal sequence is cleaved, allowing for the 248 residue SCF to properly fold and mature. If the SCF or Kit Ligand is cleaved again on the C-terminal end at residue 190, then the soluble Kit Ligand is formed. SCF, a non-covalently linked homodimer, is known to stimulate the proliferation of mast cells and is able to augment the proliferation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture. Also, SCF serves to mediate cell-cell adhesion and acts synergistically with other cytokines and interleukins.

Product Categories/Family for TNFRSF11a clia kit

Luminometer Sandwich ELISA

NCBI and Uniprot Product Information

• NCBI GI #: 17380323
• NCBI GeneID: 21934
• UniProt Accession #: O35305
• NCBI Official Full Name: Tumor necrosis factor receptor superfamily member 11A
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily, member 11a, NFKB activator
• NCBI Official Symbol: Tnfrsf11a
• NCBI Official Synonym Symbols: OFE; ODFR; Rank; Ly109; mRANK; TRANCE-R
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 11A
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 11A
• UniProt Gene Name: Tnfrsf11a
• UniProt Synonym Gene Names: Rank; ODFR
• UniProt Entry Name: TNR11_MOUSE