Tripartite motif-containing protein 72 (TRIM72) Recombinant Protein | TRIM72 recombinant protein
Recombinant Human Tripartite motif-containing protein 72 (TRIM72), partial
Gene Names
TRIM72; MG53
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Synonyms
Tripartite motif-containing protein 72 (TRIM72); N/A; Recombinant Human Tripartite motif-containing protein 72 (TRIM72), partial; Mitsugumin-53 (Mg53); (MG53); TRIM72 recombinant protein
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE.
Form/Format
Liquid or Lyophilized powder
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, p
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, p
Sequence Positions
2-477aa; Partial
Sequence
SAAPGLLHQELSCPLCLQLFDAPVTAECGHSFCRACLGRVAGEPAADGTVLCPCCQAPTRPQALSTNLQLARLVEGLAQVPQGHCEEHLDPLSIYCEQDRALVCGVCASLGSHRGHRLLPAAEAHARLKTQLPQQKLQLQEACMRKEKSVAVLEHQLVEVEETVRQFRGAVGEQLGKMRVFLAALEGSLDREAERVRGEAGVALRRELGSLNSYLEQLRQMEKVLEEVADKPQTEFLMKYCLVTSRLQKILAESPPPARLDIQLPIISDDFKFQVWRKMFRALMPALEELTFDPSSAHPSLVVSSSGRRVECSEQKAPPAGEDPRQFDKAVAVVAHQQLSEGEHYWEVDVGDKPRWALGVIAAEAPRRGRLHAVPSQGLWLLGLREGKILEAHVEAKEPRALRSPERRPTRIGLYLSFGDGVLSFYDASDADALVPLFAFHERLPRPVYPFFDVCWHDKGKNAQPLLLVGPEGAEA
Species
Human
Tag
N-terminal GST-tagged
Expression System
E.coli
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 degree C/-80 degree C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Preparation and Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20 degree C/-80 degree C.
The shelf life of lyophilized form is 12 months at -20 degree C/-80 degree C.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.
Generally, the shelf life of liquid form is 6 months at -20 degree C/-80 degree C.
The shelf life of lyophilized form is 12 months at -20 degree C/-80 degree C.
Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.
Related Product Information for TRIM72 recombinant protein
Muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites. Specifically binds phosphatidylserine. Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation. Probably acts upstream of the Ca2+-dependent membrane resealing process. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membrane budding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles
Product Categories/Family for TRIM72 recombinant protein
References
"S-nitrosylation of TRIM72 at cysteine 144 is critical for protection against oxidation-induced protein degradation and cell death."
Kohr M.J., Evangelista A.M., Ferlito M., Steenbergen C., Murphy E.J. Mol. Cell. Cardiol. 69:67-74(2014)
Kohr M.J., Evangelista A.M., Ferlito M., Steenbergen C., Murphy E.J. Mol. Cell. Cardiol. 69:67-74(2014)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
79.4kDa
NCBI Official Full Name
tripartite motif-containing protein 72
NCBI Official Synonym Full Names
tripartite motif containing 72
NCBI Official Symbol
TRIM72
NCBI Official Synonym Symbols
MG53
NCBI Protein Information
tripartite motif-containing protein 72
UniProt Protein Name
Tripartite motif-containing protein 72
UniProt Gene Name
TRIM72
UniProt Synonym Gene Names
Mg53
UniProt Entry Name
TRI72_HUMAN
Similar Products
Product Notes
The TRIM72 trim72 (Catalog #AAA243737) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 2-477aa; Partial. The amino acid sequence is listed below: SAAPGLLHQE LSCPLCLQLF DAPVTAECGH SFCRACLGRV AGEPAADGTV LCPCCQAPTR PQALSTNLQL ARLVEGLAQV PQGHCEEHLD PLSIYCEQDR ALVCGVCASL GSHRGHRLLP AAEAHARLKT QLPQQKLQLQ EACMRKEKSV AVLEHQLVEV EETVRQFRGA VGEQLGKMRV FLAALEGSLD REAERVRGEA GVALRRELGS LNSYLEQLRQ MEKVLEEVAD KPQTEFLMKY CLVTSRLQKI LAESPPPARL DIQLPIISDD FKFQVWRKMF RALMPALEEL TFDPSSAHPS LVVSSSGRRV ECSEQKAPPA GEDPRQFDKA VAVVAHQQLS EGEHYWEVDV GDKPRWALGV IAAEAPRRGR LHAVPSQGLW LLGLREGKIL EAHVEAKEPR ALRSPERRPT RIGLYLSFGD GVLSFYDASD ADALVPLFAF HERLPRPVYP FFDVCWHDKG KNAQPLLLVG PEGAEA. It is sometimes possible for the material contained within the vial of "Tripartite motif-containing protein 72 (TRIM72), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.