SDS-PAGE
(3 ug by SDS-PAGE under reducing conditions and visualized by coomasie blue stain)
TSG101 recombinant protein
TSG101, 1-145aa, Human, His tag, E Coli
Gene Names
TSG101; TSG10; VPS23
Applications
SDS-Page
Purity
> 90% by SDS-PAGE
Synonyms
TSG101; N/A; TSG101, 1-145aa, Human, His tag, E Coli; Tumor susceptibility gene 101; TSG10; VPS23; Tumor susceptibility gene 101 ESCRT I complex subunit TSG101; TSG 10; TSG 101; Tumor susceptibility gene 10; Tumor susceptibility protein; Tumor susceptibility protein isoform 3; VPS 23; VPS23.; TSG101 recombinant protein
Host
E Coli
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 10% glycerol
Concentration
0.5 mg/ml (determined by Bradford assay) (varies by lot)
Sequence Positions
1-145aa
Sequence
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMAVS ESQLKKMVSK YKYRDLTVRETVNVITLYKD LKPVLDSYVF NDGSSRELMN LTGTIPVPYR GNTYNIPICL WLLDTYPYNPPICFVKPTSS MTIKTGKHVD ANGKIYLPYL HEWKHPQSDL LGLIQVMIVV FGDEPPVFSR P
Applicable Applications for TSG101 recombinant protein
SDS-PAGE
Tag Information
His-tag
Preparation and Storage
Can be stored at 2-8°C for 1 week.
For long term storage, aliquot and store at -20°C to -80°C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20°C to -80°C.
Avoid repeated freezing and thawing cycles.
SDS-PAGE
(3 ug by SDS-PAGE under reducing conditions and visualized by coomasie blue stain)
SDS-PAGE
(3 ug by SDS-PAGE under reducing conditions and visualized by coomasie blue stain)
Related Product Information for TSG101 recombinant protein
TSG101 (Tumor susceptibility gene 101) protein belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. This protein contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. Mutations and alternative splicing in this protein occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression. Recombinant human TSG101 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for TSG101 recombinant protein
References
Vardhana S., et al. (2010) Immunity. 32 (4):531-40. ; Popov S., et al. (2009) J Virol. 83 (14):7185-93.;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
20.7 kDa (181aa), confirmed by MALDI-TOF
NCBI Official Full Name
tumor susceptibility gene 101 protein
NCBI Official Synonym Full Names
tumor susceptibility 101
NCBI Official Symbol
TSG101
NCBI Official Synonym Symbols
TSG10; VPS23
NCBI Protein Information
tumor susceptibility gene 101 protein
UniProt Protein Name
Tumor susceptibility gene 101 protein
UniProt Gene Name
TSG101
Similar Products
Product Notes
The TSG101 tsg101 (Catalog #AAA11786) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-145aa with tag His-tag. AAA Biotech's TSG101 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the TSG101 tsg101 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMAVS ESQLKKMVSK YKYRDLTVRE TVNVITLYKD LKPVLDSYVF NDGSSRELMN LTGTIPVPYR GNTYNIPICL WLLDTYPYNP PICFVKPTSS MTIKTGKHVD ANGKIYLPYL HEWKHPQSDL LGLIQVMIVV FGDEPPVFSR P. It is sometimes possible for the material contained within the vial of "TSG101, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.