Standard Curve (Sample)
Human Urinary bladder cancer antigen ELISA Kit | UBC elisa kit
Human Urinary bladder cancer antigen ELISA Kit
Gene Names
UBC; HMG20
Reactivity
Human
Synonyms
Urinary bladder cancer antigen; N/A; Human Urinary bladder cancer antigen ELISA Kit; UBC elisa kit
Reactivity
Human
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
2.5-50ng/mL
Sensitivity
0.1ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for UBC elisa kit
For Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Intended Uses: This UBC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human UBC. This ELISA kit for research use only!
Principle of the Assay: UBC ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-UBC antibody and an UBC-HRP conjugate. The assay sample and buffer are incubated together with UBC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the UBC concentration since UBC from samples and UBC-HRP conjugate compete for the anti-UBC antibody binding site. Since the number of sites is limited, as more sites are occupied by UBC from the sample, fewer sites are left to bind UBC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The UBC concentration in each sample is interpolated from this standard curve.
Intended Uses: This UBC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human UBC. This ELISA kit for research use only!
Principle of the Assay: UBC ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-UBC antibody and an UBC-HRP conjugate. The assay sample and buffer are incubated together with UBC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the UBC concentration since UBC from samples and UBC-HRP conjugate compete for the anti-UBC antibody binding site. Since the number of sites is limited, as more sites are occupied by UBC from the sample, fewer sites are left to bind UBC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The UBC concentration in each sample is interpolated from this standard curve.
Product Categories/Family for UBC elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
77,039 Da
NCBI Official Full Name
UBC protein, partial
NCBI Official Synonym Full Names
ubiquitin C
NCBI Official Symbol
UBC
NCBI Official Synonym Symbols
HMG20
NCBI Protein Information
polyubiquitin-C
UniProt Protein Name
Polyubiquitin-C
UniProt Gene Name
UBC
UniProt Entry Name
UBC_HUMAN
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Product Notes
The Human UBC ubc (Catalog #AAA86391) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA86391 ELISA Kit recognizes Human UBC. It is sometimes possible for the material contained within the vial of "Urinary bladder cancer antigen, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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