Rabbit anti-All Ubiquitin Monoclonal Antibody | anti-UBC antibody

Ubiquitin Antibody, Clone FK2

Cat. Num. AAA104166

• Gene Names: UBB; HEL-S-50
• Reactivity: All
• Applications: ELISA, Western Blot
• Purity: Protein A Purified
• Synonyms: Ubiquitin, Antibody; Ubiquitin Antibody, Clone FK2; Rabbit Anti-Human Ubiquitin Recombinant Monoclonal IgG; Polyubiquitin B Antibody; RPS27A Antibody; UBA52 Antibody; UBB Antibody; UBC Antibody; ubiquitin B Antibody; anti-UBC antibody

• Host: Rabbit
• Reactivity: All
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: FK2
• Specificity: Detects ubiquitined proteins, including poly-ubiquitinated proteins (K29-, K48-, and K63-linked mono- and polyubiquitinylated proteins). Does not detect free ubiquitin.
• Purity/Purification: Protein A Purified
• Form/Format: PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 229

• Applicable Applications for anti-UBC antibody: ELISA, WB (Western Blot)
• Target: Ubiquitin
• Immunogen: Ubiquitin conjugated lysozyme
• Cellular Localization: Cytoplasm; Nucleus
• Certificate of Analysis: A 1:1000 dilution of MBS809276 was sufficient for detection of Ubiquitin in 2 ug of purified poly-ubiquitin chains (Poly-Ubiquitin: Ub1-Ub7 WT Chains K63-linked, k48-linked) by ECL immunoblot analysis using goat anti-rabbit IgG: HRP as the secondary antibody.
• Reasearch Areas: Cell Signaling, Post-translational Modifications, Ubiquitination, Neuroscience, Neurodegeneration, Alzheimer's Disease.
• Preparation and Storage: Store at -20°C; 1 year; Avoid freeze/thaw cycles.

WB (Western Blot)

(Western Blot analysis of Purified poly-ubiquitin chains showing detection of Multiple Ubiquitin protein using Rabbit Anti-Ubiquitin Monoclonal Antibody, Clone FK2. Lane 1: Molecular Weight Ladder (MW). Lane 2: K63 Poly Ubiquitin. Lane 3: K48 Poly Ubiquitin. Load: 2 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-Ubiquitin Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: Multiple.)

Related Product Information for anti-UBC antibody

Ubiquitin is a small protein that occurs in all eukaryotic cells. The ubiquitin protein itself consists of 76 amino acids and has a molecular mass of about 8.5kDa. Key features include its C-terminal tail and the 7 Lys residues. It is highly conserved among eukaryotic species: Human and yeast ubiquitin share 96% sequence identity (1). The main function of Ubiquitin is to clear abnormal, foreign and improperly folded proteins by targeting them for degradation by the 26S proteosome (2). Ubiquitination represents an essential cellular process affected by a multi-enzyme cascade involving classes of enzymes known as ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s or Ubcs) and ubiquitin-protein ligases (E3s). Ubiquitin is activated in a two-step reaction by an E1 ubiquitin-activating enzyme in a process requiring ATP as an energy source. The initial step involves production of an ubiquitin-adenylate intermediate. The second step transfers ubiquitin to the E1 active site cysteine residue, with release of AMP. This step results in a thioester linkage between the C-terminal carboxyl group of ubiquitin and the E1 cysteine sulfhydryl group. The third step is a transfer of ubiquitin from E1 to the active site cysteine of a ubiquitin-conjugating enzyme E2 via a trans(thio)esterification reaction. And the final step of the ubiquitylation cascade creates an isopeptide bond between a lysine of the target protein and the C-terminal glycine of ubiquitin. In general, this step requires the activity of one of the hundreds of E3 ubiquitin-protein ligases (often termed simply ubiquitin ligase). E3 enzymes function as the substrate recognition modules of the system and are capable of interaction with both E2 and substrate(2, 3). Ubiquitination also participates in the internalization and degradation of plasma membrane proteins such as some of the TCR subunits while still ER-membrane associated (4). Ubiquitin also plays a role in regulating signal transduction cascades through the elimination inhibitory proteins, such as IkappaBalpha and p27 (5).

Product Categories/Family for anti-UBC antibody

Antibodies; Recombinant Monoclonal; Cell Signaling; Post-translational Modifications; Ubiquitination; Neuroscience; Neurodegeneration; Alzheimer's Disease

References

1. Wilkinson K.D. (1995) Annu. Rev. Nutr. 15:161-189. 2. Bonifacino J.S., et al. (1998) Annu Rev Cell Dev Biol. 14: 19-57. 3. Boston Biochem: "Ubiquitin Proteasome Pathway Overview" http://www.bostonbiochem.com/upp.php 4. Yang M., et al. (1998) J Exp Med. 187: 1835-1846. 5. Chen Z.J., et al. (1996) Cell 84: 853-862.

NCBI and Uniprot Product Information

• NCBI GI #: 528524471
• NCBI GeneID: 7314
• NCBI Accession #: NP_001268645.1
• NCBI GenBank Nucleotide #: NM_001281716.1
• UniProt Accession #: P0CG47; P02248; P02249; P02250; P62988; Q29120; Q6LBL4; Q6LDU5; Q8WYN8; Q91887; Q91888; Q9BWD6
• NCBI Official Full Name: polyubiquitin-B
• NCBI Official Synonym Full Names: ubiquitin B
• NCBI Official Symbol: UBB
• NCBI Official Synonym Symbols: HEL-S-50
• NCBI Protein Information: polyubiquitin-B
• UniProt Protein Name: Polyubiquitin-B
• UniProt Gene Name: UBB