Application Data
(The purity of ULK1was determined to be >70% by densitometry, approx. MW ~125kDa.)
ULK1 recombinant protein
ULK1, Active
Gene Names
ULK1; ATG1; ATG1A; UNC51; hATG1; Unc51.1
Purity
>70%
Synonyms
ULK1; N/A; ULK1, Active; ULK1 recombinant protein
Host
Sf9 insect cells using baculovirus
Purity/Purification
>70%
Form/Format
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, and 25% glycerol
Concentration
0.1 ug/ul (varies by lot)
Sequence Positions
1-649
Sequence Length
3214
Species
Human
Specific Activity
11.5 nmol/min/mg
Tag
N-terminal GST tag
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Application Data
(The purity of ULK1was determined to be >70% by densitometry, approx. MW ~125kDa.)
Application Data
(The purity of ULK1was determined to be >70% by densitometry, approx. MW ~125kDa.)
Application Data
(The specific activity of ULK1 was determined to be 11.5 nmol/min/mg as per activity assay protocol, and was equivalent to 46.3 nmol/min/mg as per radiometric assay.)
Application Data
(The specific activity of ULK1 was determined to be 11.5 nmol/min/mg as per activity assay protocol, and was equivalent to 46.3 nmol/min/mg as per radiometric assay.)
Related Product Information for ULK1 recombinant protein
Description: Recombinant human ULK1 (1-649) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. The gene accession number is BC111603.
Scientific Background: ULK1 is a serine/threonine protein kinase that plays critical role during initial stages of autophagy which is a vital response to nutrient starvation. The conserved C-terminal domain (CTD) of ULK1 controls the regulatory function and localization of the protein. Knockdown of ULK1 inhibits the autophagic response as well as inhibiting rapamycin-induced autophagy consistent with a role downstream of mTOR (1). ULK1 forms a complex with FIP200 and ATG13 and this complex is essential for starvation-induced autophagy (2). Both FIP200 and ATG13 are critical for correct localization of ULK1 to the pre-autophagosome and stability of ULK1 protein. ULK1 is phosphorylated by the mTOR pathway in a nutrient starvation-regulated manner.
Scientific Background: ULK1 is a serine/threonine protein kinase that plays critical role during initial stages of autophagy which is a vital response to nutrient starvation. The conserved C-terminal domain (CTD) of ULK1 controls the regulatory function and localization of the protein. Knockdown of ULK1 inhibits the autophagic response as well as inhibiting rapamycin-induced autophagy consistent with a role downstream of mTOR (1). ULK1 forms a complex with FIP200 and ATG13 and this complex is essential for starvation-induced autophagy (2). Both FIP200 and ATG13 are critical for correct localization of ULK1 to the pre-autophagosome and stability of ULK1 protein. ULK1 is phosphorylated by the mTOR pathway in a nutrient starvation-regulated manner.
Product Categories/Family for ULK1 recombinant protein
References
1. Ganley I G, et al: ULK1.ATG13.FIP200 complex mediates mTOR signaling and is essential for autophagy. J Biol Chem. 2009 May 1;284 (18):12297-305. 2. Chan E Y, et al: siRNA screening of the kinome identifies ULK1 as a multidomain modulator of autophagy. J Biol Chem. 2007 Aug 31;282 (35):25464-74.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Synthetic construct Homo sapiens clone IMAGE:40080552, MGC:133415 ULK1 protein (ULK1) mRNA, encodes complete protein
NCBI Official Synonym Full Names
unc-51 like autophagy activating kinase 1
NCBI Official Symbol
ULK1
NCBI Official Synonym Symbols
ATG1; ATG1A; UNC51; hATG1; Unc51.1
NCBI Protein Information
serine/threonine-protein kinase ULK1
UniProt Protein Name
Serine/threonine-protein kinase ULK1
UniProt Gene Name
ULK1
UniProt Synonym Gene Names
KIAA0722; ATG1; hATG1
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Product Notes
The ULK1 ulk1 (Catalog #AAA14258) is a Recombinant Protein produced from Sf9 insect cells using baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-649. It is sometimes possible for the material contained within the vial of "ULK1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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