USP5 recombinant protein

USP5 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA27763

• Gene Names: USP5; ISOT
• Purity: >90% as determined by SDS-PAGE
• Synonyms: USP5; N/A; USP5 Protein, Human, Recombinant (His Tag); Human USP5/ISOT Protein (His Tag); ISOT Protein, Human; ubiquitin specific peptidase 5 (isopeptidase T); USP5 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 150mM NaCl, 10% Glycerol, pH 7.5, 5%Trehalose, 5%Mannitol, 0.01% Tween-80

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Met 1
• Tag: His
• Protein Construction: A DNA sequence encoding the human USP5 isoform short (P45974-2)(Met 1-Ser 835) was fused with a polyhistidine tag at the C-terminus.
• Reconstitution: It is recommended that sterile water (400ul) be added to the vial to prepare a stock solution of 0.25mg/mL. Concentration is measured by UV-Vis.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70°C.; Store it under sterile conditions at -20°C to -80°C upon receiving.; Recommend to aliquot the protein into smaller quantities for optimal storage.; Avoid repeated freeze-thaw cycles.

SDS-PAGE

Related Product Information for USP5 recombinant protein

Background: Ubiquitin carboxyl-terminal hydrolase 5, also known as Deubiquitinating enzyme 5, Isopeptidase T, Ubiquitin thiolesterase 5, Ubiquitin-specific-processing protease 5, ISOT and USP5, is a member of the peptidase C19 family. USP5 contains 2 UBA domains and one UBP-type zinc finger. The UBP-type zinc finger domain interacts selectively with an unmodified C-terminus of the proximal ubiquitin. Both UBA domains are involved in polyubiquitin recognition. The UBP-type zinc finger domain crystallizes as a dimer linked by a disulfide bond between the Cys-195 residues of both molecules, but there is no evidence that the full-length USP5 exists as a dimer. USP5 cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. USP5 is involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. It binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.

References

Reyes-Turcu F.E., et al., 2006, Cell 124:1197-1208. Reyes-Turcu F.E., et al., 2008, J. Biol. Chem. 283:19581-19592. Dayal S., et al., 2009, J. Biol. Chem. 284:5030-5041. Burkard T.R., et al., 2011, BMC Syst. Biol. 5:17-17.

NCBI and Uniprot Product Information

• NCBI GI #: 1717869
• NCBI GeneID: 8078
• UniProt Accession #: P45974; Q96J22; D3DUS7; D3DUS8
• Molecular Weight: The recombinant human USP5 consists of 846 amino acids and predicts a molecular mass of 94.7 kDa. It migrates as an approximately 100 kDa band in SDS-PAGE under reducing conditions.
• NCBI Official Full Name: Ubiquitin carboxyl-terminal hydrolase 5
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 5 (isopeptidase T)
• NCBI Official Symbol: USP5
• NCBI Official Synonym Symbols: ISOT
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 5; isopeptidase T; ubiquitin isopeptidase T; ubiquitin thioesterase 5; deubiquitinating enzyme 5; ubiquitin thiolesterase 5; ubiquitin-specific-processing protease 5; ubiquitin specific protease 5 (isopeptidase T); ub
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 5
• UniProt Gene Name: USP5
• UniProt Synonym Gene Names: ISOT
• UniProt Entry Name: UBP5_HUMAN