SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
USP5 recombinant protein
USP5, Active
Applications
Western Blot
Synonyms
USP5; N/A; USP5, Active; ISOT; Isopeptidase T; USP5 recombinant protein
Host
E Coli
Form/Format
Recombinant protein stored in 50mM sodium phosphate, pH7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
Applicable Applications for USP5 recombinant protein
WB (Western Blot), Activity Assay
Tag
HIS
Species
Human
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
SDS-PAGE
(Purity: Sample Purity Data. For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet.)
Activity
(Specific Activity: Sample Activity Plot. For specific information on a given lot, see related technical data sheet.)
Related Product Information for USP5 recombinant protein
Recombinant full-length human USP5 was expressed in E Coli cells using an N-terminal His tag. The USP5 gene accession number is NM_003481.
USP5 (ubiquitin carboxyl-terminal hydrolase 5) is a member of the ubiquitin-specific protease family of deubiquitinating enzymes (DUBs) and is involved in many processes including maintenance of chromatin structure, receptor function, and degradation of abnormal proteins. USP5 disassembles branched polyubiquitin chains by a sequential exo mechanism at the proximal end of the chain. Increased USP5 has been associated with tumour formation and progression of pancreatic cancer due to its role in FoxM1 protein stabilization (1). USP5 is also involved in the upregulation of Notch signaling and downregulation of receptor tyrosine kinase signaling in Drosophila eye development through the deubiquitination of unanchored polyubiquitin (2).
USP5 (ubiquitin carboxyl-terminal hydrolase 5) is a member of the ubiquitin-specific protease family of deubiquitinating enzymes (DUBs) and is involved in many processes including maintenance of chromatin structure, receptor function, and degradation of abnormal proteins. USP5 disassembles branched polyubiquitin chains by a sequential exo mechanism at the proximal end of the chain. Increased USP5 has been associated with tumour formation and progression of pancreatic cancer due to its role in FoxM1 protein stabilization (1). USP5 is also involved in the upregulation of Notch signaling and downregulation of receptor tyrosine kinase signaling in Drosophila eye development through the deubiquitination of unanchored polyubiquitin (2).
Product Categories/Family for USP5 recombinant protein
References
1. Li, X.Y. et al: USP5 promotes tumorigenesis and progression of pancreatic cancer by stabilizing FoxM1 protein. Biochem. Biophys. Res. Commun. 492:48-54, 2017.
2. Ling, X. et al: The deubiquitinating enzyme Usp5 regulates Notch and RTK signaling during Drosophila eye development. FEBS 591(6): 875-888, 2017.
2. Ling, X. et al: The deubiquitinating enzyme Usp5 regulates Notch and RTK signaling during Drosophila eye development. FEBS 591(6): 875-888, 2017.
NCBI and Uniprot Product Information
Similar Products
Product Notes
The USP5 (Catalog #AAA74096) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's USP5 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), Activity Assay. Researchers should empirically determine the suitability of the USP5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "USP5, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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