Application Data
(HTRF assay for VRK1 activity STK S1 substrate was incubated with different concentrations of VRK1 protein in a reaction system for 1 hour. The detection reagents were added and incubated with the reactions for 1 hr. All the operations and reactions were performed at room temperature, and HTRF KinASE STK assay was used to detect the enzymatic activity.)
VRK1 recombinant protein
Recombinant VRK1 protein
Gene Names
VRK1; PCH1; PCH1A
Synonyms
VRK1; N/A; Recombinant VRK1 protein; PCH1; PCH1A; VRK1 recombinant protein
Host
Baculovirus
Form/Format
Recombinant VRK1 protein is supplied in 25mM HEPES-NaOH pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100 and 0.5mM TCEP.
Source
Human
Protein Note
Recombinant human VRK1 protein was expressed in a baculovirus expression system as the full length protein (accession number NP_003375.1) with a N-terminal DYKDDDDK tag. The molecular weight of the protein is
46.7 kDa
Notes
This protein is suitable for use in binding assays, inhibitor screening, and selectivity profiling. Kinase Activity Assay Conditions: 1uM STK S1 substrate was incubated with different concentrations of VRK1 protein in 10ul reaction system containing 1×Enzymatic Buffer, 5mM MgCl2, 1mM DTT and 100uM ATP for 1 hour. The 10ul detection reagents containing STK antibody and SA-XL665, each of which was 1:100 diluted with 1×Detection Buffer were added and incubated with the reactions for 1 hr. All the operations and reactions were performed at room temperature, and HTRF KinASE STK assay was used to detect the enzymatic activity.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
Application Data
(HTRF assay for VRK1 activity STK S1 substrate was incubated with different concentrations of VRK1 protein in a reaction system for 1 hour. The detection reagents were added and incubated with the reactions for 1 hr. All the operations and reactions were performed at room temperature, and HTRF KinASE STK assay was used to detect the enzymatic activity.)
Application Data
(HTRF assay for VRK1 activity STK S1 substrate was incubated with different concentrations of VRK1 protein in a reaction system for 1 hour. The detection reagents were added and incubated with the reactions for 1 hr. All the operations and reactions were performed at room temperature, and HTRF KinASE STK assay was used to detect the enzymatic activity.)
Application Data
(HTRF assay for VRK1 activity STK S1 substrate was incubated with different concentrations of VRK1 protein in a reaction system for 1 hour. The detection reagents were added and incubated with the reactions for 1 hr. All the operations and reactions were performed at room temperature, and HTRF KinASE STK assay was used to detect the enzymatic activity.)
Application Data
(HTRF assay for VRK1 activity STK S1 substrate was incubated with different concentrations of VRK1 protein in a reaction system for 1 hour. The detection reagents were added and incubated with the reactions for 1 hr. All the operations and reactions were performed at room temperature, and HTRF KinASE STK assay was used to detect the enzymatic activity.)
SDS-PAGE
(Recombinant VRK1 protein gel 10% SDS-PAGE Coomassie staining MW: 46.7 kDa Purity: >90% )
SDS-PAGE
(Recombinant VRK1 protein gel 10% SDS-PAGE Coomassie staining MW: 46.7 kDa Purity: >90% )
Related Product Information for VRK1 recombinant protein
Background: VRK1 (Vaccinia Related Kinase 1), also known as PCH1A, is a serine/threonine kinase. It is involved in Golgi disassembly during the cell cycle, by which following phosphorylation by PLK3 during mitosis, it is required to induce Golgi fragmentation. VRK1 acts by mediating phosphorylation of downstream target protein. It can phosphorylate Thr-18 of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. It phosphorylates BANF1, thus disrupts its ability to bind DNA, reduces its binding to LEM domain-containing proteins and causes its relocalization from the nucleus to the cytoplasm. VRK1 also phosphorylates casein and histone H3, as well as ATF2 which activates its transcriptional activity.
Product Categories/Family for VRK1 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
46.7 kDa
NCBI Official Full Name
serine/threonine-protein kinase VRK1
NCBI Official Synonym Full Names
vaccinia related kinase 1
NCBI Official Symbol
VRK1
NCBI Official Synonym Symbols
PCH1; PCH1A
NCBI Protein Information
serine/threonine-protein kinase VRK1
UniProt Protein Name
Serine/threonine-protein kinase VRK1
UniProt Gene Name
VRK1
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Product Notes
The VRK1 vrk1 (Catalog #AAA60130) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "VRK1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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