Standard Curve (Sample)
Mouse wingless-type MMTV integration site family, member 5A ELISA Kit | WNT5A elisa kit
Mouse Protein Wnt-5a, WNT5A ELISA Kit
Gene Names
Wnt5a; Wnt-5a; 8030457G12Rik
Reactivity
Mouse
Synonyms
wingless-type MMTV integration site family, member 5A; N/A; Mouse Protein Wnt-5a, WNT5A ELISA Kit; Mouse Protein Wnt-5a (WNT5A) ELISA kit; hWNT5A; WNT-5A protein; wingless-type MMTV integration site family; member 5A; WNT5A elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse WNT5A. No significant cross-reactivity or interference between mouse WNT5A and analogues was observed.
Sequence Length
360
Samples
Serum, plasma, cell culture supernates, tissue homogenates
Assay Type
Sandwich
Detection Range
3.12 pg/ml -200 pg/ml.
Sensitivity
The minimum detectable dose of mouse WNT5A is typically less than 0.78 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for WNT5A elisa kit
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for WNT5A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any WNT5A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WNT5A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WNT5A bound in the initial step. The color development is stopped and the intensity of the color is measured.
References
Ah Receptor Activation by Dioxin Disrupts Activin, BMP, and WNT Signals During the Early Differentiation of Mouse Embryonic Stem Cells and Inhibits Cardiomyocyte Functions. Qin Wang, Hisaka Kurita, Vinicius Carreira, Chia-I Ko, Yunxia Fan, Xiang Zhang, Jacek Biesiada, Mario Medvedovic and Alvaro Puga (2015)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42,309 Da
NCBI Official Full Name
protein Wnt-5a isoform 2
NCBI Official Synonym Full Names
wingless-type MMTV integration site family, member 5A
NCBI Official Symbol
Wnt5a
NCBI Official Synonym Symbols
Wnt-5a; 8030457G12Rik
NCBI Protein Information
protein Wnt-5a; wingless-related MMTV integration site 5A
UniProt Protein Name
Protein Wnt-5a
UniProt Gene Name
Wnt5a
UniProt Synonym Gene Names
Wnt-5a
UniProt Entry Name
WNT5A_MOUSE
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Product Notes
The Mouse WNT5A wnt5a (Catalog #AAA112824) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA112824 ELISA Kit recognizes Mouse WNT5A. It is sometimes possible for the material contained within the vial of "wingless-type MMTV integration site family, member 5A, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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