Application Data
Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) Recombinant Protein | WNT5A recombinant protein
Recombinant Wingless Type MMTV Integration Site Family, Member 5A (WNT5A)
Gene Names
Wnt5a; Wnt-5a; 8030457G12Rik
Reactivity
Mouse
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation, Immunoprecipitation
Purity
> 95%
Synonyms
Wingless Type MMTV Integration Site Family; Member 5A (WNT5A); Recombinant Wingless Type MMTV Integration Site Family; WNT5A recombinant protein
Host
E Coli
Reactivity
Mouse
Purity/Purification
> 95%
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300
Concentration
200ug/mL (varies by lot)
Applicable Applications for WNT5A recombinant protein
SDS-PAGE, WB, ELISA, IP, CoIP, ReporterAssays, Purification, Amine Reactive Labeling.
(May be suitable for use in other assays to be determined by the end user.)
(May be suitable for use in other assays to be determined by the end user.)
Source
Prokaryotic expression
Residues
Ile62~Lys380
Tags
N-terminal GST-tag
Tissue Specificity
Cerebellum
Subcellular Location
Secreted, extracellular space, extracellular matrix
Traits
Freeze-dried powder
Predicted isoelectric point
8.8
Predicted Molecular Mass
61.8kDa
Accurate Molecular Mass
62kDa as determined by SDS-PAGE reducing conditions
Usage
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition.
Application Data
Application Data
SDS-PAGE
SDS-PAGE
Sequence Information
Sequence Information
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
protein Wnt-5a isoform 2
NCBI Official Synonym Full Names
wingless-related MMTV integration site 5A
NCBI Official Symbol
Wnt5a
NCBI Official Synonym Symbols
Wnt-5a; 8030457G12Rik
NCBI Protein Information
protein Wnt-5a
UniProt Protein Name
Protein Wnt-5a
UniProt Gene Name
Wnt5a
UniProt Synonym Gene Names
Wnt-5a
UniProt Entry Name
WNT5A_MOUSE
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Product Notes
The WNT5A wnt5a (Catalog #AAA20255) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Wingless Type MMTV Integration Site Family, Member 5A (WNT5A) can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, WB, ELISA, IP, CoIP, ReporterAssays, Purification, Amine Reactive Labeling. (May be suitable for use in other assays to be determined by the end user.) . Researchers should empirically determine the suitability of the WNT5A wnt5a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Wingless Type MMTV Integration Site Family, Member 5A (WNT5A), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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