IGF1 active protein

IGF1 Protein, Mouse, Recombinant

Cat. Num. AAA258162

• Gene Names: Igf1; Igf-1; Igf-I; C730016P09Rik
• Purity: >95% as determined by SDS-PAGE
• Synonyms: IGF1; N/A; IGF1 Protein, Mouse, Recombinant; Mouse IGF1/IGF?I/IGF-1 protein; C730016P09Rik Protein, Mouse; Igf-1 Protein, Mouse; Igf-I Protein, Mouse; insulin-like growth factor 1 (somatomedin C); IGF1 active protein

• Host: Yeast
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Gly49-Ala118

• Species: Mouse
• Activity: Measured in a cell proliferation assay using MCF-7 cells. The ED50 for this effect is 0.2-1.2ug/mL.
• Predicted N Terminal: Gly 49
• Tag: Native
• Protein Construction: A DNA sequence encoding the mouse IGF1 (NP_001104744.1)(Gly49-Ala118) was expressed.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

Application Data

(Measured in a cell proliferation assay using MCF-7 cells. The ED50 for this effect is 0.2-1.2 ug/mL.)

SDS-PAGE

Related Product Information for IGF1 active protein

Background: IGF I, also known as Mechano Growth Factor, somatomedin-C, IGF-I, and IGF1, is a secreted protein that belongs to the insulin family. The insulin family, comprised of insulin, relaxin, insulin-like growth factors I and II (IGF-I and IGF-II), and possibly the beta-subunit of 7S nerve growth factor, represents a group of structurally related polypeptides whose biological functions have diverged. The IGFs, or somatomedins, constitute a class of polypeptides that have a key role in pre-adolescent mammalian growth. IGF-I expression is regulated by GH and mediates postnatal growth, while IGF-II appears to be induced by placental lactogen during prenatal development. IGF1/IGF-I may be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. IGF1/IGF-I stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also about enhancing glucose uptake. Defects in IGF1/IGF-I are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) which is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness, and mental retardation.

Product Categories/Family for IGF1 active protein

Serine Proteases and Regulators

References

Jansen M., et al.,(1983), Sequence of cDNA encoding human insulin-like growth factor I precursor. Nature 306:609-611.de Pagter-Holthuizen P., et al., (1986), Organization of the human genes for insulin-like growth factors I and II.FEBS Lett. 195:179-184.le Bouc Y., et al.,(1986), Complete characterization of the human IGF-I nucleotide sequence isolated from a newly constructed adult liver cDNA library.FEBS Lett. 196:108-112.

NCBI and Uniprot Product Information

• NCBI GI #: 163659889
• NCBI GeneID: 16000
• NCBI Accession #: NP_001104744.1
• NCBI GenBank Nucleotide #: NP_001104744.1
• UniProt Accession #: Q8CAR0; P05017
• Molecular Weight: 7.7
• NCBI Official Full Name: insulin-like growth factor I isoform 3
• NCBI Official Synonym Full Names: insulin-like growth factor 1
• NCBI Official Symbol: Igf1
• NCBI Official Synonym Symbols: Igf-1; Igf-I; C730016P09Rik
• NCBI Protein Information: insulin-like growth factor I
• UniProt Protein Name: Insulin-like growth factor I
• UniProt Gene Name: Igf1