Mouse Zinc finger E-box-binding homeobox 1 ELISA Kit | ZEB1 elisa kit
Mouse Zinc finger E-box-binding homeobox 1 (ZEB1) ELISA Kit
Gene Names
Zeb1; Tw; BZP; ZEB; MEB1; Nil2; Tcf8; AREB6; TCF-8; Tcf18; Zfhep; Zfx1a; Zfhx1a; Zfx1ha; [delta]EF1; 3110032K11Rik
Reactivity
Mouse
Synonyms
Zinc finger E-box-binding homeobox 1; N/A; Mouse Zinc finger E-box-binding homeobox 1 (ZEB1) ELISA Kit; ZEB1 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse ZEB1. No significant cross-reactivity or interference between Mouse ZEB1 and analogues was observed.
Sequence Length
1124
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for ZEB1 elisa kit
TCF8 encodes a human zinc finger transcription factor that represses T-lymphocyte-specific IL2 gene expression by binding to a negative regulatory domain 100 nucleotides 5-prime of the IL2 transcription start site.
Delta-Ef1 selectively transactivated the promoters of the SMC differentiation marker genes Acta2 and Myh11, and it physically interacted with Srf and Smad3, resulting in synergistic activation of the Acta2 promoter. Chromatin immunoprecipitation assays and knockdown experiments showed that delta-Ef1 was involved in control of SMC differentiation programs induced by Tgf-beta (TGFB1) signaling. Overexpression of delta-Ef1 inhibited neointima formation and promoted SMC differentiation.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ZEB1 in samples. An antibody specific for ZEB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZEB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZEB1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZEB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Delta-Ef1 selectively transactivated the promoters of the SMC differentiation marker genes Acta2 and Myh11, and it physically interacted with Srf and Smad3, resulting in synergistic activation of the Acta2 promoter. Chromatin immunoprecipitation assays and knockdown experiments showed that delta-Ef1 was involved in control of SMC differentiation programs induced by Tgf-beta (TGFB1) signaling. Overexpression of delta-Ef1 inhibited neointima formation and promoted SMC differentiation.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ZEB1 in samples. An antibody specific for ZEB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZEB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZEB1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZEB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
122,465 Da
NCBI Official Full Name
zinc finger E-box-binding homeobox 1
NCBI Official Synonym Full Names
zinc finger E-box binding homeobox 1
NCBI Official Symbol
Zeb1
NCBI Official Synonym Symbols
Tw; BZP; ZEB; MEB1; Nil2; Tcf8; AREB6; TCF-8; Tcf18; Zfhep; Zfx1a; Zfhx1a; Zfx1ha; [delta]EF1; 3110032K11Rik
NCBI Protein Information
zinc finger E-box-binding homeobox 1
UniProt Protein Name
Zinc finger E-box-binding homeobox 1
UniProt Gene Name
Zeb1
UniProt Synonym Gene Names
Tcf8; Zfhx1a; Zfx1a; Zfx1ha; TCF-8; MEB1
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Product Notes
The Mouse ZEB1 zeb1 (Catalog #AAA55351) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA55351 ELISA Kit recognizes Mouse ZEB1. It is sometimes possible for the material contained within the vial of "Zinc finger E-box-binding homeobox 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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