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Zika virus envelope Recombinant Protein

Zika virus envelope protein

Applications
ELISA, Western Blot
Purity
95% by SDS-PAGE
Synonyms
Zika virus envelope; N/A; Zika virus envelope protein; Zika envelope protein; Zika protein; recombinant Zika envelope protein; ZIKV envelope; Zika ENV protein; Zika virus E protein; Zika virus antigen; recombinant Zika protein; recombinant Zika antigen; Zika virus envelope antigen; recombinant Zika envelope antigen; ZIKV protein; ZIKV antigen; Zika virus envelope recombinant protein
Ordering
For Research Use Only!
Host
E. coli
Purity/Purification
95% by SDS-PAGE
Form/Format
Frozen liquid supplied in 50mM NaCl, 50mM NaPO4, pH 8.5, 0.02% SDS
Concentration
1.0 mg/ml (varies by lot)
Applicable Applications for Zika virus envelope recombinant protein
ELISA (EIA), Western Blot (WB)
Application Notes
ELISA: 1-2 ug/ml protein range for titering Dengue virus converted human polyclonal antibodies.
Lateral Flow: 1-2mg/ml (1-2ul/cm).
Grade
Recombinant
Species
Viral
Method of Purification
Purified by aqueous solvent extraction and preparative electrophoresis to > 95% purity.
Biohazard
Use standard laboratory precautions when handling this material.
Additional Remarks
This protein binds to Zika converted human serum polyclonal antibodies and cross reacts with Dengue virus converted human serum polyclonal antibodies.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Ships on dry ice. Upon receipt, aliquot and store at -70°C.
Related Product Information for Zika virus envelope recombinant protein
Biological Significance: Zika virus was first discovered in Ughanda in 1947 in monkeys infected by the virus through bites from of infected Aedes species mosquitos. Zika virus has become a highly researched disease due to increase in incidences from 2007 to 2016, when the virus spread eastward, across the Pacific Ocean to the Americas. In 2015–16 Zika virus epidemic reached pandemic levels. The Zika envelope protein composes the majority of the virion surface and it is involved with replication, such as host cell binding and membrane fusion.

Implications in Disease: The symptoms of Zika infection vary from non-existant to visible rash, fever, headaches and conjunctivitis. It is usually non life threatening, but has been associated to microcephaly in unborn fetuses when passed by an infected pregnant mother.
References
Fetal Growth Restriction Caused by Sexual Transmission of Zika Virus in Mice . Ryuta Uraki, Kellie Ann Jurado, Jesse Hwang, Klara Szigeti-Buck, Tamas L. Horvath, Akiko Iwasaki, Erol Fikrig (2017)

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Product Notes

The Zika virus envelope (Catalog #AAA24015) is a Recombinant Protein produced from E. coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Zika virus envelope can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). ELISA: 1-2 ug/ml protein range for titering Dengue virus converted human polyclonal antibodies. Lateral Flow: 1-2mg/ml (1-2ul/cm). Researchers should empirically determine the suitability of the Zika virus envelope for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Zika virus envelope, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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