Bioactivity
(The epidermal growth factor receptor (EGFR) is a growth factor receptor that induces cell differentiation and proliferation upon activation through the binding of one of its ligands. The receptor is located at the cell surface, where the binding of a ligand activates a tyrosine kinase in the intracellular region of the receptor. This tyrosine kinase phosphorylates a number of intracellular substrates that activates pathways leading to cell growth, DNA synthesis and the expression of oncogenes such as fos and jun. Epidermal growth factor (EGF) family peptides are ligands for the EGF receptor (EGFR), thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human EGFR and recombinant human EGF. Briefly, biotin-linked EGFR were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to EGF-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 ul stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant human EGFR and recombinant human EGF was shown in Figure 1, the EC50 for this effect is 11.46 ug/mL.)
Epidermal Growth Factor Receptor (EGFR) Active Protein | EGFR active protein
Active Epidermal Growth Factor Receptor (EGFR)
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Synonyms
Epidermal Growth Factor Receptor (EGFR); N/A; Active Epidermal Growth Factor Receptor (EGFR); Epidermal Growth Factor Receptor; EGFR active protein (eukaryotic,293F cell), Human; ErbB-1; ErbB1; ER1; ERB-B1; MENA; HER1; Erythroblastic Leukemia Viral(v-Erb-B)oncogene Homolog,Avian; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-14; EGFR active protein
Host
293F cell
Purity/Purification
Greater than 90% by SDS-PAGE
Form/Format
Freeze-dried powder; PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Sequence
Leu25~Ser645
Applicable Applications for EGFR active protein
Activity Assay, Cell Culture
Species
Human
Expression System
Eukaryotic expression
Tag
N-terminal His Tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Isoelectric Point
6.5
Research Area
CD & Adhesion molecule, Tumor immunity
Preparation and Storage
Store at 2-8 degree C for one month, -80 degree C for 12 months
Bioactivity
(The epidermal growth factor receptor (EGFR) is a growth factor receptor that induces cell differentiation and proliferation upon activation through the binding of one of its ligands. The receptor is located at the cell surface, where the binding of a ligand activates a tyrosine kinase in the intracellular region of the receptor. This tyrosine kinase phosphorylates a number of intracellular substrates that activates pathways leading to cell growth, DNA synthesis and the expression of oncogenes such as fos and jun. Epidermal growth factor (EGF) family peptides are ligands for the EGF receptor (EGFR), thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human EGFR and recombinant human EGF. Briefly, biotin-linked EGFR were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to EGF-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 ul stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant human EGFR and recombinant human EGF was shown in Figure 1, the EC50 for this effect is 11.46 ug/mL.)
Bioactivity
(The epidermal growth factor receptor (EGFR) is a growth factor receptor that induces cell differentiation and proliferation upon activation through the binding of one of its ligands. The receptor is located at the cell surface, where the binding of a ligand activates a tyrosine kinase in the intracellular region of the receptor. This tyrosine kinase phosphorylates a number of intracellular substrates that activates pathways leading to cell growth, DNA synthesis and the expression of oncogenes such as fos and jun. Epidermal growth factor (EGF) family peptides are ligands for the EGF receptor (EGFR), thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human EGFR and recombinant human EGF. Briefly, biotin-linked EGFR were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to EGF-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 ul stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant human EGFR and recombinant human EGF was shown in Figure 1, the EC50 for this effect is 11.46 ug/mL.)
SDS-PAGE
(Figure. SDS-PAGE)
SDS-PAGE
(Figure. SDS-PAGE)
Product Categories/Family for EGFR active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
134,277 Da
NCBI Official Full Name
epidermal growth factor receptor isoform a
NCBI Official Synonym Full Names
epidermal growth factor receptor
NCBI Official Symbol
EGFR
NCBI Official Synonym Symbols
ERBB; HER1; mENA; ERBB1; PIG61
NCBI Protein Information
epidermal growth factor receptor; proto-oncogene c-ErbB-1; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; receptor tyrosine-protein kinase erbB-1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog
UniProt Protein Name
Epidermal growth factor receptor
UniProt Gene Name
EGFR
UniProt Synonym Gene Names
ERBB; ERBB1; HER1
UniProt Entry Name
EGFR_HUMAN
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Product Notes
The EGFR egfr (Catalog #AAA161774) is an Active Protein produced from 293F cell and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Epidermal Growth Factor Receptor (EGFR) can be used in a range of immunoassay formats including, but not limited to, Activity Assay, Cell Culture. Researchers should empirically determine the suitability of the EGFR egfr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Leu25~Ser6 45. It is sometimes possible for the material contained within the vial of "Epidermal Growth Factor Receptor (EGFR), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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