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IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using XRCC4 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
Rabbit XRCC4 Polyclonal Antibody | anti-XRCC4 antibody
XRCC4 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Synonyms
XRCC4; Polyclonal Antibody; XRCC4 Polyclonal Antibody; DNA repair protein XRCC4; X-ray repair cross-complementing protein 4; anti-XRCC4 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Form/Format
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Concentration
1mg/ml (varies by lot)
Sequence Length
336
Applicable Applications for anti-XRCC4 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IHC: 1:50 - 1:200
Species
Human
Immunogen
Recombinant Protein
Immunogen
Recombinant protein of human XRCC4
Calculated Molecular Weight
38kDa
Preparation and Storage
Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using XRCC4 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence)
(Immunofluorescence analysis of U2OS cells using XRCC4 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded human B cell lymphoma using XRCC4 Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistchemistry)
(Immunohistochemistry of paraffin-embedded human B cell lymphoma using XRCC4 Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded rat pancreas using XRCC4 Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded rat pancreas using XRCC4 Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human gastric cancer using XRCC4 Antibody at dilution of 1:200 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human gastric cancer using XRCC4 Antibody at dilution of 1:200 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human B cell lymphoma using XRCC4 Antibody at dilution of 1:200 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded human B cell lymphoma using XRCC4 Antibody at dilution of 1:200 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded rat pancreas using XRCC4 Antibody at dilution of 1:200 (40x lens).)
IHC (Immunohistochemistry)
(Immunohistochemistry of paraffin-embedded rat pancreas using XRCC4 Antibody at dilution of 1:200 (40x lens).)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using XRCC4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using XRCC4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
Related Product Information for anti-XRCC4 antibody
The protein encoded by this gene functions together with DNA ligase IV and the DNA-dependent protein kinase in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. The non-homologous end-joining pathway is required both for normal development and for suppression of tumors. This gene functionally complements XR-1 Chinese hamster ovary cell mutant, which is impaired in DNA double-strand breaks produced by ionizing radiation and restriction enzymes. Alternative transcription initiation and alternative splicing generates several transcript variants. [provided by RefSeq, Sep 2008]
Product Categories/Family for anti-XRCC4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
336
NCBI Official Full Name
DNA repair protein XRCC4
NCBI Official Synonym Full Names
X-ray repair complementing defective repair in Chinese hamster cells 4
NCBI Official Symbol
XRCC4
NCBI Protein Information
DNA repair protein XRCC4; X-ray repair cross-complementing protein 4; X-ray repair, complementing defective, repair in Chinese hamster
UniProt Protein Name
DNA repair protein XRCC4
UniProt Gene Name
XRCC4
UniProt Entry Name
XRCC4_HUMAN
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Product Notes
The XRCC4 xrcc4 (Catalog #AAA10755) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The XRCC4 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's XRCC4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500 - 1:2000 IHC: 1:50 - 1:200. Researchers should empirically determine the suitability of the XRCC4 xrcc4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "XRCC4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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