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IF (Immunofluorescence)
(Immunofluorescence of RSPO1 in human spleen tissue with RSPO1 antibody at 5 μg/ml.Green: RSPO1 antibody (5171)Red: Phylloidin stainingBlue: DAPI staining)
Rabbit RSPO1 Polyclonal Antibody | anti-RSPO1 antibody
RSPO1 Antibody
Gene Names
RSPO1; RSPO; CRISTIN3
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
RSPO1 Antibody is affinity chromatography purified via peptide column.
Synonyms
RSPO1, Antibody; RSPO1 Antibody; RSPO1; RSPO; CRISTIN3; R-spondin-1; Roof plate-specific spondin-1; hRspo1; R-spondin homolog (Xenopus laevis); anti-RSPO1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
RSPO1 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
263
Applicable Applications for anti-RSPO1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
RSPO1 antibody can be used for detection of RSPO1 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.
Conjugate
Unconjugated
Immunogen
RSPO1 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human RSPO1.
Buffer
RSPO1 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
Antibody can be stored at 4 degree C up to one year. Antibodies should not be exposed to prolonged high temperatures.
IF (Immunofluorescence)
(Immunofluorescence of RSPO1 in human spleen tissue with RSPO1 antibody at 5 μg/ml.Green: RSPO1 antibody (5171)Red: Phylloidin stainingBlue: DAPI staining)
IF (Immunofluorescence)
(Immunofluorescence of RSPO1 in human spleen tissue with RSPO1 antibody at 5 μg/ml.Green: RSPO1 antibody (5171)Red: Phylloidin stainingBlue: DAPI staining)
IHC (Immunohistochemistry)
(Immunohistochemistry of RSPO1 in human spleen tissue with RSPO1 antibody at 5 μg/ml.)
IHC (Immunohistochemistry)
(Immunohistochemistry of RSPO1 in human spleen tissue with RSPO1 antibody at 5 μg/ml.)
IHC (Immunohistochemistry)
(Immunohistochemistry of RSPO1 in human spleen tissue with RSPO1 antibody at 2.5 μg/mL.)
IHC (Immunohistochemistry)
(Immunohistochemistry of RSPO1 in human spleen tissue with RSPO1 antibody at 2.5 μg/mL.)
IF (Immunofluorescence)
(Immunofluorescence of RSPO1 in Human Spleen tissue with RSPO1 antibody at 20 μg/mL.)
IF (Immunofluorescence)
(Immunofluorescence of RSPO1 in Human Spleen tissue with RSPO1 antibody at 20 μg/mL.)
IHC (Immunohistochemistry)
(Immunohistochemistry of RSPO1 in human heart tissue with RSPO1 antibody at 2.5 μg/ml.)
IHC (Immunohistochemistry)
(Immunohistochemistry of RSPO1 in human heart tissue with RSPO1 antibody at 2.5 μg/ml.)
WB (Western Blot)
(Western blot analysis of RSPO1 in human heart tissue lysate with RSPO1 antibody at (A) 1 and (B) 2 μg/mL.)
WB (Western Blot)
(Western blot analysis of RSPO1 in human heart tissue lysate with RSPO1 antibody at (A) 1 and (B) 2 μg/mL.)
Related Product Information for anti-RSPO1 antibody
RSPO1 Antibody: RSPO1 is a member of a family of secreted growth factors that can operate through the canonical Wnt signaling pathway by stabilizing the intracellular beta-catenin, thereby regulating functions mediated by beta-catenin such as cell fate decisions and embryonic patterning. RSPO1 was recently identified through linkage analysis to be involved in sex determination and mammalian ovarian development. RSPO1 is thought to regulate cellular responsiveness to Wnt ligands by modulating the cell-surface expression of the Wnt co-receptor LRP6 by interfering with the DKK/Kremen-mediated internalization of LRP6 through an interaction with Kremen, resulting in increased LRP6 cell-surface levels. At least two isoforms of RSPO1 are known to exist. RSPO1 antibody will not cross-react with RSPO family members.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21,775 Da
NCBI Official Full Name
R-spondin-1 isoform 1
NCBI Official Synonym Full Names
R-spondin 1
NCBI Official Symbol
RSPO1
NCBI Official Synonym Symbols
RSPO; CRISTIN3
NCBI Protein Information
R-spondin-1; R-spondin homolog; roof plate-specific spondin-1
UniProt Protein Name
R-spondin-1
UniProt Gene Name
RSPO1
UniProt Synonym Gene Names
hRspo1
UniProt Entry Name
RSPO1_HUMAN
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Product Notes
The RSPO1 rspo1 (Catalog #AAA10920) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RSPO1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RSPO1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). RSPO1 antibody can be used for detection of RSPO1 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the RSPO1 rspo1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RSPO1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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