<
WB (Western Blot)
(Applications: Western BlotSample: Rat PFCDetection: Protein Simple, Simple Western size-based assay"We selected this product as a polyclonal ab for our Tg Rat colony. We are interested in adding enzymatic catabolism/clearance of amyloid beta isoforms into our body of research. We purchased this product because it was cheap and we are looking at some 'proof-of-concept' and didn't need remarkable specificity. We still have much to investigate.")
Rabbit CD10 Polyclonal Antibody | anti-MME antibody
Anti-CD10 Antibody
Gene Names
MME; NEP; SFE; CD10; CALLA
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Synonyms
CD10; Polyclonal Antibody; Anti-CD10 Antibody; Neprilysin; membrane metallo-endopeptidase; Atriopeptidase antibody; CALLA antibody; CD10 antibody; CD10 antigen antibody; Common acute lymphocytic leukemia antigen antibody; DKFZp686O16152 antibody; EC 3.4.24.11 antibody; Enkephalinase antibody; EPN antibody; gp100 antibody; Membrane metallo endopeptidase (neutral endopeptidase; enkephalinase) antibody; enkephalinase; CALLA; CD10) antibody; Membrane metallo endopeptidase antibody; Membrane metallo endopeptidase variant 1 antibody; Membrane metallo endopeptidase variant 2 antibody; Membrane metalloendopeptidase antibody; Membrane metalloendopeptidase neutral endopeptidase enkephalinase antibody; Membrane metalloendopeptidase neutral endopeptidase enkephalinase CALLA CD10 antibody; Membrane metalloendopeptidase variant 1 antibody; Membrane metalloendopeptidase variant 2 antibody; MGC126681 antibody; MGC126707 antibody; MME antibody; NEP antibody; NEP_HUMAN antibody; Neprilysin antibody; Neutral endopeptidase 24.11 antibody; Neutral endopeptidase antibody; SFE antibody; Skin fibroblast elastase antibody; anti-MME antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
750
Applicable Applications for anti-MME antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Immunogen
E Coli-derived human CD10 recombinant protein (Position: Y52-W750). Human CD10 shares 94% amino acid (aa) sequences identity with both mouse and rat CD10.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
WB (Western Blot)
(Applications: Western BlotSample: Rat PFCDetection: Protein Simple, Simple Western size-based assay"We selected this product as a polyclonal ab for our Tg Rat colony. We are interested in adding enzymatic catabolism/clearance of amyloid beta isoforms into our body of research. We purchased this product because it was cheap and we are looking at some 'proof-of-concept' and didn't need remarkable specificity. We still have much to investigate.")
WB (Western Blot)
(Applications: Western BlotSample: Rat PFCDetection: Protein Simple, Simple Western size-based assay"We selected this product as a polyclonal ab for our Tg Rat colony. We are interested in adding enzymatic catabolism/clearance of amyloid beta isoforms into our body of research. We purchased this product because it was cheap and we are looking at some 'proof-of-concept' and didn't need remarkable specificity. We still have much to investigate.")
Application Data
(Anti-CD10 Picoband antibody, AAA11606-5.jpgAll lanes: Anti-CD10(AAA11606) at 0.5ug/mlLane 1: Rat Kidney Tissue Lysate at 40ugLane 2: Rat Brain Tissue Lysate at 40ugLane 3: Rat Liver Tissue Lysate at 40ugLane 4: Human Placenta Tissue Lysate at 40ugLane 5: HELA Whole Cell Lysate at 40ugLane 6: JURKAT Whole Cell Lysate at 40ugLane 7: RAJI Whole Cell Lysate at 40ugLane 8: 293T Whole Cell Lysate at 40ugPredicted bind size: 86KDObserved bind size: 100KD)
Application Data
(Anti-CD10 Picoband antibody, AAA11606-5.jpgAll lanes: Anti-CD10(AAA11606) at 0.5ug/mlLane 1: Rat Kidney Tissue Lysate at 40ugLane 2: Rat Brain Tissue Lysate at 40ugLane 3: Rat Liver Tissue Lysate at 40ugLane 4: Human Placenta Tissue Lysate at 40ugLane 5: HELA Whole Cell Lysate at 40ugLane 6: JURKAT Whole Cell Lysate at 40ugLane 7: RAJI Whole Cell Lysate at 40ugLane 8: 293T Whole Cell Lysate at 40ugPredicted bind size: 86KDObserved bind size: 100KD)
IHC (Immunohistochemistry)
(Anti-CD10 Picoband antibody, AAA11606-4.JPGIHC(P): Rat Spleen Tissue)
IHC (Immunohistochemistry)
(Anti-CD10 Picoband antibody, AAA11606-4.JPGIHC(P): Rat Spleen Tissue)
IHC (Immunohistochemistry)
(Anti-CD10 Picoband antibody, AAA11606-3.JPGIHC(P): Mouse Spleen Tissue)
IHC (Immunohistochemistry)
(Anti-CD10 Picoband antibody, AAA11606-3.JPGIHC(P): Mouse Spleen Tissue)
IHC (Immunohistochemistry)
(Anti-CD10 Picoband antibody, AAA11606-2.JPGIHC(P): Human B lymphocyte Cancer Tissue)
IHC (Immunohistochemistry)
(Anti-CD10 Picoband antibody, AAA11606-2.JPGIHC(P): Human B lymphocyte Cancer Tissue)
IHC (Immunohistochemistry)
(Anti-CD10 Picoband antibody, AAA11606-1.JPGIHC(P): Mouse Kidney Tissue)
IHC (Immunohistochemistry)
(Anti-CD10 Picoband antibody, AAA11606-1.JPGIHC(P): Mouse Kidney Tissue)
Related Product Information for anti-MME antibody
Description: Rabbit IgG polyclonal antibody for Neprilysin(MME) detection. Tested with WB, IHC-P in Human, Mouse, Rat.
Background: CD10, also known as membrane metallo-endopeptidase, neutral endopeptidase(NEP), Neprilysin, or common acute lymphoblastic leukemia antigen(CALLA), is a zinc-dependent metalloprotease enzyme that degrades a number of small secreted peptides, most notably theamyloid beta peptide whose abnormal misfolding and aggregation in neural tissue has been implicated as a cause of Alzheimer's disease. This gene is localized to human chromosome 3 by study of somatic cell hybrids and regionalized the location to 3q21-q27 by in situ hybridization. By cDNA transfection analysis, CD10 is confirmed as a functional neutral endopeptidase of the type that has previously been called enkephalinase. CD10 has also been called atriopeptidase. Atriopeptidase specifically degrades atrial natriuretic factor. A specific enzyme inhibitor was developed and reported that it had effects similar to those of low-dose ANF infusion. These effects include diuresis, natriuresis, vasodilatation, and suppression of the renin-angiotensin-aldosterone system.
Background: CD10, also known as membrane metallo-endopeptidase, neutral endopeptidase(NEP), Neprilysin, or common acute lymphoblastic leukemia antigen(CALLA), is a zinc-dependent metalloprotease enzyme that degrades a number of small secreted peptides, most notably theamyloid beta peptide whose abnormal misfolding and aggregation in neural tissue has been implicated as a cause of Alzheimer's disease. This gene is localized to human chromosome 3 by study of somatic cell hybrids and regionalized the location to 3q21-q27 by in situ hybridization. By cDNA transfection analysis, CD10 is confirmed as a functional neutral endopeptidase of the type that has previously been called enkephalinase. CD10 has also been called atriopeptidase. Atriopeptidase specifically degrades atrial natriuretic factor. A specific enzyme inhibitor was developed and reported that it had effects similar to those of low-dose ANF infusion. These effects include diuresis, natriuresis, vasodilatation, and suppression of the renin-angiotensin-aldosterone system.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
85,514 Da
NCBI Official Full Name
Neprilysin
NCBI Official Synonym Full Names
membrane metallo-endopeptidase
NCBI Official Symbol
MME
NCBI Official Synonym Symbols
NEP; SFE; CD10; CALLA
NCBI Protein Information
neprilysin; enkephalinase; atriopeptidase; neprilysin-390; neprilysin-411; skin fibroblast elastase; neutral endopeptidase 24.11; membrane metallo-endopeptidase variant 1; membrane metallo-endopeptidase variant 2; common acute lymphocytic leukemia antigen; membrane metallo-endopeptidase (neutral endopeptidase, enkephalinase); membrane metallo-endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10)
UniProt Protein Name
Neprilysin
UniProt Gene Name
MME
UniProt Synonym Gene Names
EPN; CALLA; NEP; Neutral endopeptidase; SFE
UniProt Entry Name
NEP_HUMAN
Similar Products
Product Notes
The MME mme (Catalog #AAA11606) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD10 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CD10 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. Researchers should empirically determine the suitability of the MME mme for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
