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Application Data
(Staining of mouse peripheral blood lymphocytes with Rat anti MouseE CD49d: Low Endotoxin)
Rat CD49d Monoclonal Antibody | anti-CD49d antibody
RAT ANTI MOUSE CD49d:Low Endotoxin
Gene Names
Itga4; CD49D; Itga4B
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Functional Assay, Immunoprecipitation
Synonyms
CD49d, Antibody; RAT ANTI MOUSE CD49d:Low Endotoxin; anti-CD49d antibody
Host
Rat
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
PS/2
Form/Format
Low Endotoxin
Purified IgG - liquid
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (varies by lot)
Sequence Length
1,039
Applicable Applications for anti-CD49d antibody
Immunohistology Frozen, Flow cytometry (FC/FACS), Functional Assays (FN), Immunoprecipitation (IP)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100
Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Target Species
Mouse
Preparation and Storage
Store at -20 degree C only. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Application Data
(Staining of mouse peripheral blood lymphocytes with Rat anti MouseE CD49d: Low Endotoxin)
Application Data
(Staining of mouse peripheral blood lymphocytes with Rat anti MouseE CD49d: Low Endotoxin)
Application Data
(Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD49d: Alexa Fluor 647)
Application Data
(Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD49d: Alexa Fluor 647)
Application Data
(Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD49d: FITC)
Application Data
(Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD49d: FITC)
Application Data
(Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD49d)
Application Data
(Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD49d)
Application Data
(Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD49d)
Application Data
(Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD49d)
Application Data
(Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD49d: Azide Free)
Application Data
(Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD49d: Azide Free)
Related Product Information for anti-CD49d antibody
Rat anti Mouse CD49d monoclonal antibody, clone PS/2 recognizes murine alpha 4 integrin (CD49d), a ~150kDa single pass type I membrane glycoprotein that can associate with either beta 1 integrin (CD29) or beta 7 integrin to form heterodimers CD49d/CD29 (VLA-4) and alpha4/beta7 (LPAM-1) respectively (Holzmann et al. 1989). CD49d is expressed on most lymphocytes, granulocytes, monocytes and thymocytes. The primary ligands for CD49d are CD106 (VCAM-1), fibronectin and MAdCAM-1 (Sheppard et al. 1994). Clone PS/2 has also been reported to block the binding of CD49d to its ligands (Andrew et al. 1994).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
115,696 Da
NCBI Official Full Name
integrin alpha-4
NCBI Official Synonym Full Names
integrin alpha 4
NCBI Official Symbol
Itga4
NCBI Official Synonym Symbols
CD49D; Itga4B
NCBI Protein Information
integrin alpha-4; CD49 antigen-like family member D; LPAM subunit alpha; VLA-4 receptor, alpha 4 subunit; VLA-4 subunit alpha; integrin alpha-IV; integrin alpha4B; lymphocyte Peyer patch adhesion molecules subunit alpha
UniProt Protein Name
Integrin alpha-4
UniProt Gene Name
Itga4
UniProt Synonym Gene Names
LPAM subunit alpha
UniProt Entry Name
ITA4_MOUSE
Similar Products
Product Notes
The CD49d itga4 (Catalog #AAA11898) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD49d can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, Flow cytometry (FC/FACS), Functional Assays (FN), Immunoprecipitation (IP). Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100. Researchers should empirically determine the suitability of the CD49d itga4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD49d, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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